Bacterial Toxin Research Citations

EAE Induction:

EAE was induced 23 weeks following transfer of memory cells. Recipient mice or untransferred controls were immunized…in four sites on the back and received 100 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive transfer of T-cell subsets or whole lymphocytes:

Treatment with pertussis toxin (PTX) was performed by incubating 4 10⁷ cells/mL in RPMI containing 0.1M N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid and 2% fetal calf serum with 100 ng/mL PTX (List Biological Laboratories, Campbell, CA) for 2 hours at 37C, and washed >4 times.  Donor cells were labeled with 5 M of either carboxyfluorescein diacetate succinimidyl ester…and intravenously injected into host mice.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Two intraperitoneal injections of 250 ng pertussis toxin (List Biologicals, Campbell, CA) were administered on days 0 and 2. Mice were observed and scored according to disease severity, where a score of 0 = no disease, 1 = limp tail, 2 = hind-limb paresis, 3 = hind-limb …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Functional Characterization of Peritoneal and Bone Marrow-derived Macrophages:

Peritoneal macrophages (p-m) were harvested as described above. …For experiments macrophages were cultured at a density of 0.510⁶ cells/mL and were incubated for 24 hours in the absence or presence of lipopolysaccharide (LPS; 50 ng/mL; Salmonella Minnesota R595 (Re); List Biological Laboratories Inc. Campbell, CA)…

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Experimental Autoimmune Encephalomyelitis:

Female mice were immunized by subcutaneous administration with 200 m g of MOG 3555 peptide (Invitrogen) emulsified in IFA (Difco) containing 500 m g of TDM on day 0, supplemented with intravenous injection of 200 ng of pertussis toxin (List Biological Laboratories). Mice received an additional intraperitoneal injection of 200 ng pertussis toxin on day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…For ELISA, 50 L of a 5 g/mL solution of anthrax PA, activated PA63 (List Biological Labs, Campbell, CA, USA), was dissolved in carbonate-bicarbonate buffer and used to coat each well of a 96-well plate …’

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… The following primary antibodies are recommended: goat antibody against CTB (1:1,000, No. 703; List Biological Laboratories, Campbell, CA, USA) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Cell Culture, Proteins, Antibodies and Chemicals:

…Cholera toxin was obtained from List Biological Laboratory.

Immunofluorescence Staining and Confocal Laser Scanning Microscopy:

…Cells without CARDS toxin were processed similarly to serve as negative controls. In parallel, cells treated with cholera toxin (0.5 µg) or Tf (10 µg), plus their respective antibodies at 1:500 dilution, served as positive controls for caveolin-mediated and clathrin-mediated endocytosis.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunization EAE:

Mice were immunized subcutaneously at the base of the tail with 50 g MOG₃₅₅₅ peptide (Schafer-N, Copenhagen, Denmark) in PBS emulsified in complete Freunds adjuvant (CFA). To induce the development of EAE, MOG-peptide immunized C57Bl/B6 mice were also injected twice (on the same day and two days later) with Pertussis toxin (200 ng; List Biological Laboratories, Inc. Campbell, Ca) in PBS.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… CT was purchased from List Biological Laboratories (Campbell, Calif.). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae