Bacterial Toxin Research Citations

… A commercially available rLF used as control was purchased from List Biological Laboratories. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

… Animals were immunized via the intranasal route with 5 µg cholera toxin (CT; List Biological Laboratories, Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunization:

Once weekly for 3 consecutive weeks, female BALB/c mice were immunized intranasally with cCHP-PspA, PspA plus CT (1 µg; List Biological Laboratory, Campbell, CA), PspA alone, or phosphate-buffered saline (PBS) only. Some experiments included an irrelevant antigen as a control; …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Table 1. Antibody Characterization:

Cholera toxin subunit B, Purified CTB isolated from Vibrio cholerae, List Biological Laboratories (Campbell, CA), goat polyclonal #703, 1:5,000. …

Histology:

Table 1 lists all antibodies used. The CTB antibody was tested on galago brain tissue with no CTB injections and this control failed to label any cells or patches of axon terminals.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Table 1. Antibody Characterization: 

…List Biological Laboratories (Campbell, CA), goat polyclonal #703, 1:5,000. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Neonatal LPS injections and hypoxic-ischemic brain injury:

All experiments were performed on C57/Bl6 mice…Mice were given a 0.3 mg/kg or 1.0 mg/kg single intraperitoneal injection (i.p.) of LPS (ultra pure E. coli 055:B5, List biological laboratories, Inc) at postnatal day 9…

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Reagents:

…LPS (R595Re) and pertussis toxin were purchased from List biological. …

Results

Zymosan induces increases in cAMP and activation of PKA in wild type BMDMs:

…Activation of the TLR3 or TLR4 pathway with their respective ligands, poly I:C or LPS, did not acutely affect the intracellular concentration of cAMP (up to 15 minutes post ligand addition). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

In vitro mast cellactivation assay:

For activation assays, 2.5 3 105 BMCMCs were plated/well in a 96-well U-bottom plate. Virus was added at a multiplicity of infection (MOI) 1, and the nal volume was brought to 100 ml and incubated for 46 h. As positive controls, BMCMCs were stimulated with LPS (5 mg/ml; List Biologicals Laboratory) for cytokine/chemokine release or the calcium ionophore A23187…

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… microglial cells were plated per well in 12-well plates in DMEM supplemented as above but in 2% FBS, and was left to settle for 24 h. Thereafter, the medium was changed to DMEM without serum, and with or without 100 nM lipopolysaccharide (LPS) (List biological lab). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Isolation and stimulation of neonatal rat CFB:

…Following 24 h starvation from serum, the cells were stimulated for 24 h with vehicle (sterile NaCl/Pi), 10 ngmL 1 IL-1b (PRC0814; Gibco), 25 ngmL 1 IL-6 (406-ML; R&D Systems, Minneapolis, MN, USA), 1 lgmL 1 LPS (201; List Biological Laboratories, Campell, CA, USA) or …

• Product #201 – LPS from Escherichia coli O111:B4