Bacterial Toxin Research Citations

… EAE model, as previously described (Yarim et al. 2022). After (MOG)(35-55) injection, intraperitoneal injections of 200 ng pertussis toxin (List Biological Laboratories, USA) in the first hour and 400 ng pertussis toxin in 200 µl PBS were administered at the hour 48. The experimental design of the groups and EAE induction procedure are shown in Fig. 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tract tracing and immunohistochemistry

For tracer injections, bats were anesthetized by isoflurane inhalation in a custom stereotaxic apparatus. A 1mm x 1mm craniotomy was made over the primary motor cortex. A tracer deposit via iontophoretic injection (5μA, 10 min) at varying depths was made targeting the deep layers of the primary motor cortex using 10% biotinylated dextran amine (BDA; 10,000 MW) (Life Technologies) (n=4, males) or 1% cholera toxin subunit B (CTB, List Biological Laboratories) in nucleus ambiguus/brainstem (n=2, males). …

The primary antibody solution contained of goat anti-ChAT (Millipore AB144P, 1:200) and anti-CTB (List Biological Laboratories, 1:10,000) antibodies, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Staphylococcal Enterotoxin Type B (SEB) (List Biological Laboratories Cat#122) was a positive assay control, …

EAE induction

Female mice (7–10 weeks old) were prepared for acclimatisation at least 1 week before the experiment and immunised with an emulsion containing 150 μg of I-Ab MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; MBL Life science, Japan, cat#: TS-704-P) and 500 μg of Mycobacterium tuberculosis extract H37RA in complete Freund’s adjuvant (Chondrex, WA, USA, cat#: 7023) by subcutaneous injection on the both sides of the back. 400 µg of Pertussis toxin (List Biological Laboratories, CA, USA, cat#: 180) diluted in D-PBS (nakarai tesque, cat#: 14249-24) was intraperitoneally administrated at 0- and 48-h postimmunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

2.1.1. Experimental Materials

The following materials were used: anthrax protective antigen (PA), from List Labs in California, USA, stored at 4 °C; anti-protective antigen (Anti-PA) monoclonal antibody, from List Labs in California, USA, stored at 4 °C; …

… Pertussis toxin (300 ng per 200 µL; 180, List Biological Laboratories) was intraperitoneally injected on days 0 and 2 after MOG immunization. Daily monitoring of EAE mice …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction of EAU model

On day 0, we injected mice subcutaneously with a 1:1 mixture of 200 μg retinal antigen interphotoreceptor retinoid-binding protein 1–20 (IRBP1–20) (amino acid sequence: GPTHLFQPSLVLDMAKVLLD; GL Biochem, Shanghai, China) and complete Freund’s adjuvant (Difco, Detroit, MI, USA) containing 2.5 mg/mL of Mycobacterium tuberculosis strain H37Ra (BD Difco, San Jose, CA, USA). After immunization, 0.25 ug pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in phosphate-buffered saline (PBS) was injected intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Primary antisera contained antibodies raised against PHAL (species: rabbit; dilution: 1:4,000; Vector Labs Cat # AS-2224); RRID: AB_2313686) or CTB (species: goat; dilution: 1:10,000; List Biological Cat # 703; RRID: AB_10013220) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.5. Experimental human endotoxemia

On days 0 and 7, experimental endotoxemia was performed according to our standardized protocol8. Briefly, subjects were admitted to the research unit of the Radboud university medical center for eight hours, where a radial artery catheter (BD Infusion Therapy Systems) and antebrachial venous cannula were placed to allow serial blood sampling, hemodynamic monitoring and administration of fluids and LPS, respectively. In the 45 minutes prior to LPS administration, hydration fluids (2.5% glucose/0.45% sodium chloride) were administered as a 1.5L prehydration bolus to reduce the risk of vasovagal collapse13, and thereafter at a rate of 150 mL/h for the remainder of the experiment. Directly after prehydration, a bodyweight-adjusted bolus dose of 1 ng/kg LPS (E. Coli-derived, Type O113, lot no. 94332B1; List Biological Laboratories) was administered.

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

2.2. EAE induction and assessment

EAE was triggered using a four-point paraspinal subcutaneous injection of an emulsion containing complete Freund’s adjuvant (CFA) (0.05 mL, Sigma, St Louis, USA), myelin oligodendrocyte glycoprotein (MOG) 35–55 peptide (250 μg, Peptide Biotechnology, Nanjing, China), 0.05 mL of sterilized saline and Mycobacterium tuberculosis H37Ra (4 mg/mL, Difco Laboratories, Detroit, USA); subsequently, pertussis toxin (500 ng, List Biological, CA, USA) was given through intraperitoneal injection, and again the same amount was injected after 48 h. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)