Citations

Cell cultures and drug treatments

Non-cancerous MCF10A cell and tumorigenic MCF10AT and MCF10CA1α cells were cultured in complete DMEM/F-12 medium (Gibco™) with 5% horse serum (Gibco™), 10 µg/ml Insulin (Sigma-Aldrich), 20 ng/ml Epi- dermal Growth Factor (Sigma-Aldrich), 0.5 µg/ml Hydro- cortisone (Sigma-Aldrich,), and 100 ng/ml Cholera toxin (List Biological Laboratories). MDA-MB-231 cells were cultured in complete DMEM medium (Gibco™) with 10% foetal bovine serum (Thermo Scientific). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin (List Biological Laboratories Inc., Campbell, CA) was injected on days 0 and 2 (100 ng per mouse, intraperito- neally). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… List Biological Laboratories (Campbell, California, USA) Tetanus toxoid from Clostridium tetani/191B …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Reagents

Purified lipopolysaccharide (LPS) from Escherichia coli from List Biological Laboratory (Campbell, CA, USA) was prepared in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… 50 ng/mL rhM-CSF plus 10 ng/mL lipopolysaccharide from Escherichia coli O55:B5 (LPS; List Biological Laboratories, Campbell, CA, USA) for M1, …

• Product #203A – LPS from Escherichia coli O55:B5

… Azide-free CT was purchased from List Biologicals Inc. (Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

1. Animals and cell culture

… Lipopolysaccharides (LPSs) were purchased from List Biological Laboratories Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water from Sigma-Aldrich (St. Louis, MO, USA). …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Induction and assessment of EAE

This modeling method is based on previous research [37]. Eight-week-old female C57BL/6 mice were injected subcutaneously in the dorsal flanks with 150 μg MOG35-55 peptide (RS Synthesis) emulsified in CFA (5 μg/mL). Pertussis toxin (500 ng per mouse, list labs) was given i.v. 24 h later. A second MOG and pertussis injection was given 7 d later. LND was intraperitoneally injected daily (60 mg/kg) beginning at disease induction. Mice in the vehicle group were injected with β-CD at the same time points. Animals were monitored and scored daily until they were euthanized. Clinical scores were assessed as follows: 0 = no symptoms, 1 = loss of tail tonicity, 2 = unsteady gait, 3 = hind limb paralysis, 4 = forelimb paralysis, 5 = moribund or dead. If scores fell between two points, 0.5 gradations were used.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

PBMC isolation and culture for cytokine production

… PBMC cultures were stimulated with final concentration of 5 ng/mL lipopolysaccharide (LPS) (List Biological, Campbell, CA; from E. coli 0111:B4 and dissolved in endotoxin-free water) in complete Russ-10 medium; endotoxin free water was used as a negative control. …

• Product #201 – LPS from Escherichia coli O111:B4

EAE induction and clinical scoring

Two weeks post-AAV vector delivery, EAE was induced in C57BL/6J female mice as previously described.8 Emulsion containing 150 μg of MOG33-55 (AnaSpec) in complete Freund’s adjuvant (CFA) (MilliporeSigma) with 600 μg of Mycobacterium tuberculosis (BD) was subcutaneously injected into both sides of the lateral abdomen. A 375 ng of pertussis toxin (List Biologicals) was injected intraperitoneally on days 0 and 2. For healthy controls, the same emulsion without MOG33-55 peptide was used. EAE clinical symptoms were monitored on a daily basis following Hooke lab’s Mouse EAE scoring guidelines.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)