Bacterial Toxin Research Citations

Materials:

… Pertussis toxin from List biological laboratories, Cat. No. 179A (Campbell, CA), …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Vaccine formulations:

The vaccines discussed in this report were composed of rPA (List Biological Laboratories), recombinant spore-specific proteins produced as described earlier ( Brahmbhatt et al., 2007 ; Cybulski et al., 2008 ; Mikesell et al., 1983 ) or a combination of the antigens. The specific amounts of antigens used in the vaccine formulations are described in the figure legend of each experiment. Aluminium hydroxide gel (AL; aluminium content per vaccination ~125 g for mice or ~500 g for guinea pigs) or the Sigma adjuvant system (SAS) was used as adjuvant (Sigma Aldrich). SAS is an emulsion of monophosphoryl lipid A and trehalose dicorynomycolate, which may promote mixed T helper 1 (Th1)/Th2 immune responses to vaccines, and was used as directed by the manufacturer.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Cholera toxin and insulin were from List Biological (Campbell, CA, USA) and Invitrogen (Carlsbad, CA, USA), respectively. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… SNAPtide(o-Abz-Dnp) was purchased from List Biological Laboratory. …

SNAPtide based LC activity assay:

SNAPtide (o-Abz-Dnp) (List Biological Laboratories) was used for studying the LCs
activity and inhibition (Figure 2.2). 100 M SNAPtide stock was prepared in DMSO.
The proteolytic reaction was initiated by the addition of LC to 20 mM HEPES (pH 7.5)
with 2 M SNAPtide. The protease activity was monitored by the increase of o-Abz
fluorescence (ex: 320 nm, em: 420 nm). The results were analyzed using Sigmaplot. …

• Product #520 –  SNAPtide Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

Cell culture and stimulation:

… RAW 264.7 cells expressing G_i2 and -galactosidase were stimulated with LPS (10ng/ml, ultra-pure LPS from Escherichia coli O111:B4, List Laboratories, Campbell, CA), Pam3CSK4 (1g/ml, Invivogen) or Poly (I:C) (10g/ml Invivogen) for 24 hours. LPS-induced TNF and IL-6 production were analyzed by enzyme-linked immunosorbant assay (ELISA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents and cell culture:

LPS (R595) (List Biological Laboratories, Inc., Campbell, CA, USA), …

Flow cytometry:

… For intracellular cytokine staining, cells were incubated with LPS (100ngml¹) in the presence of GolgiStop (2M; BD Biosciences) for 5h. Surface and intracellular cytokine staining were performed as described elsewhere.²⁵ In some experiments, BM cells were stimulated with LPS (100ngml¹) or Pam3CS(K)4 (100ngml¹) for 24h in the absence or presence of anti-TNF antibody (10gml¹), and were subjected to intracellular cytokine staining.

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Cell Culture:

Primary murine tracheal epithelial cells (MTECs) were isolated and cultured … Sixteen hours before treatment, cells were starved in medium containing 0.5% FBS. Cells were exposed to 1 g/ml LPS (List Biological Laboratories, Inc., Campbell, CA), …

Mice

C57Bl/6 mice were anesthetized and received 5 g LPS oropharyngeally (List Biological Laboratories, Inc.), and 24 hours later were killed by pentobarbital injection. The right lobe was flash-frozen in liquid nitrogen and pulverized, and the left lobe was inflated with 4% paraformaldehyde. Five-micron sections were used to assess the localization of RelB in situ via immunofluorescence, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Plasmids and RNAi:

… Depletion of Rae1 was performed using a double-stranded siRNA oligonucleotide with the sequence 5-CUCAGCAGUAACCAAGCGAUACAGA-3 (Rae1 siRNA; Stealth RNAi; Invitrogen). Cells were incubated with pertussis toxin (181214A1; List Biological Laboratories, Inc.) at 400 ng/ml for 4 h before analysis.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

In vitro antibody activity and aggregation assays:

… To monitor ligand-binding activity, an indirect PTx ELISA was employed as reported previously^18,37. High-binding ELISA plates (Costar) were coated with pertussis toxin (PTx, List Biological Laboratories) at 0.75 µg/ml in PBS and incubated at 4°C overnight. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Demyelination model:

We created relapsing-remitting EAE (R-EAE) from C57Bl/6 mice (Charles-River). Each mouse was immunized subcutaneously (into the flanks) with 100µg of MOG35-55 (MEVGWYRSPFSRVVHLYRNGK), 400 µg of Mycobacterium tuberculosis (strain H37Ra; Difco) in complete Freunds adjuvant (CFA, Difco). One hundred nanogram of Bordetella pertussis toxin (List Biological Laboratories via Cedarlane) was given intravenously at the time of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)