Citations

EAE Induction:

EAE was induced by subcutaneous injection of myelin oligodendrocyte glycoprotein peptide (MOG₃₅₅₅; MEVGWYRSPFSRVVHLYRNGK) injection (9). … Each mouse was injected s.c. with 100 µL MOG₃₅₅₅ emulsion and injected i.p. with 100 µL pertussis toxin (200 ng; List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… LPS (#201 from E.coli 0111:D4) was from List Biological Laboratories. …

Plaque Assays:

For plaquing, confluent monolayers of macrophages in 12-well plates were infected at an MOI of 0.01 with log phase WT Lm similar to the previously described method (Sun et al., 1990). 1 hr later, cells were washed twice with PBS and gent (10 g/ml) was added along with nothing or 4 ng/ml LPS.

• Product #201 – LPS from Escherichia coli O111:B4

Infections and EAE induction:

… EAE was induced 35-40 days post infection by injecting subcutaneously each mouse with 100 µl of emulsified complete Freund’s adjuvant (DIFCO) with 200 µg of MOG 3555 (GenWay biotech, purity >95%) and 400 µg of dessicated Mycobacterium tuberculosis H37ra (DIFCO). Mice also received two i.p. injections with 200 ng of pertussis toxin (List Biologicals) at time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PTx and PTxB were purchased from List Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell isolation:

PBMC were isolated from heparinized venous blood of healthy volunteers by Ficoll-Paque Plus (Ge-Healthcare) density gradient centrifugation as recommended by the manufacturer. Monocytes were isolated from PBMC by MACS with the Monocyte Isolation Kit II (Miltenyi) and their purity was determined by FACS by staining the cells with anti-CD14 and anti-CD45.  … Monocytes were treated with 2 µg/ml Bordetella Pertussis toxin (List Biological Laboratories) washed and subjected to video microscopy.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Evaluation of EAE:

Six- to eight-wk-old C57Bl/6 mice were immunized subcutaneously with 100µl of an emulsion containing 100µg of rMOG and 200µg of Mycobacterium tuberculosis H37Ra (BD Biosciences, San Jose, CA) distributed over three sites on the flank. Mice were injected intraperitoneally with 200ng of pertussis toxin (PTX) (List Biological Laboratories, Cambell, CA) on days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Canine keratinocyte culture medium:

… cholera toxin (List Biological Laboratories). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… LPS (Salmonella minnesota R595) was from List Biological Laboratories. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Lipid Extraction from 15-KA Spiked and EAE Mouse Serum:

To produce spiked serum samples, 15-KA was dissolved in DMSO, then added to the serum harvested from nave mice by cardiac puncture to reach the indicated concentrations and incubated overnight with mixing at 4°C. To produce EAE mouse serum, mice were injected subcutaneously with 100 mg MOG3555 and 1 mg heat-killed Mycobacterium tuberculosis H37RA (Invitrogen, Carlsbad, CA, USA) emulsified in complete Freunds adjuvant. Pertussis toxin (200 ng in PBS; List Biological Laboratories, USA) was injected intra-peritoneally on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Procedures 

Mice, Virus, and DT Administration:

… Diphtheria toxin (100 ng) (List Biological Laboratories, Campbell, CA) was administered intraperitoneally on days 5 and 7 after LCMV-Cl 13 infection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae