Bacterial Toxin Research Citations

Pre-incubation of MoDCs with toxins and chemicals:

MoDCs were incubated with PA (1 g/ml) and EF and/or LF (both from List Biological Laboratories Campbell, CA, USA) at 40 ng/ml, …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #178A – Anthrax Edema Factor (EF), Recombinant from B. anthracis

Peanut-induced anaphylaxis (PIA):

PIA was induced as previously described[5]. Briefly, mice were sensitized by oral gavage with 1 mg peanut protein (Kraft Naturals peanut butter) and 10 µg cholera toxin (List Biological Laboratories) in 100 µL sterile dH2O, weekly for 4 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… CT was purchased from List Biologicals (Campbell, CA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 10. Pertussis toxin (List Biological Labs). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… TMBPGFP blast cells (2 days after re-stimulation) were cultured for 1 h in the presence of 100 ng ml21 of PTX (List Biological Laboratories) or its B subunit (Calbiochem). These cells were used for transfer EAE. The chosen dose of PTX was able to block CXCL11- and CCL5-mediated trans-well transmigration of in-vitro- and ex-vivo-isolated TMBPGFP cells. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Oligodeoxynucleotides and reagents:

… Ultra pure lipopolysaccharide (LPS; from Escherichia coli 0111:B4) was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

Transfection and reporter gene assays:

RAW264.7 cells (2 10⁶ cells/well) were plated into 6-well plates and then incubated for 24 hr to reach approximately 80% confluence. Cells were co-transfected with pRL-TK-luciferase (1 g) and wild type or mutant Irak-m promoter-luciferase reporter constructs (3 g) using Lipofectamine (Invitrogen) according to the manufacturer’s protocol. … Transfected cells were incubated for 6 hr, pooled, and washed 3 times with culture media. Cells (1 or 210⁵ cells/well in 96-well culture plates) were stimulated with medium, CpG DNA (6 g/ml), non-CpG DNA (6 g/ml), LPS (50 ng/ml), …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

… S. minnesota ReLPS was from List Biological Laboratories (Campbell, CA, USA). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Lipopolysaccharide (LPS) was purchased from List Biological Laboratories. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

2.2.2. Cell Culture

HEK293 human embryonic kidney cells (gift of Frank Graham) were maintained
in Minimum Essential Medium (MEM) supplemented with 10% fetal bovine serum
(FBS). This cell line has been transformed with the E1 region of type 5
adenovirus (Graham et al., 1977). NIH3T3 murine embryonic fibroblasts (ATCC
#CRL-1658) were maintained in high glucose Dulbecco’s Modified Eagle Medium
(DMEM) supplemented with 10% FBS. hTERT-HME1 human mammary
epithelial cells (ATCC #CRL-4010) were maintained in DMEM/F12 supplemented
with 10 mM HEPES, 10 µg/mL insulin, 5 mg/mL BSA, 10 ng/mL Cholera toxin, 10
ng/mL epidermal growth factor (EGF), and 0.5 µg/mL hydrocortisone. MCF-10A
human mammary epithelial cells (ATCC #CRL10317) were maintained in
modified DMEM/F12 with 5% FBS, 10 µg/mL insulin, 10 ng/mL Cholera toxin, 20
ng/mL EGF, and 0.5 µg/mL hydrocortisone. MTHJ murine mammary
adenocarcinoma cells were established as described (Hummel, Safroneeva, and
Mossman, 2005) and maintained in high glucose Dulbecco’s Modified Eagle
Medium (DMEM) supplemented with 10% FBS. ZR-75-1 human breast cancer
cells (ATCC #CRL-1500) were cultured in RPMI 1640 supplemented with 10%
FBS. All cell lines were supplemented with 2 mM L-glutamine, 0.1 mg/mL
penicillin, and 100 U/mL streptomycin, and grown at 37ºC in a humidified
atmosphere at 5% CO2. All media were purchased from Sigma Chemicals and
all supplements were purchased from Gibco except for cholera toxin (List
Biologicals).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mice and EAE Induction:

… Lgals1^/ and WT mice were immunized subcutaneously in two sites (left and right flanks) with 150µg of MOG_35-55 peptide that was emulsified in complete Freunds adjuvant (CFA; Sigma-Aldrich, St. Louis) containing 200µg Mycobacterium tuberculosis (Difco, Detroit). Mice received 200 ng pertussis toxin (PT; List Biological, Campbell, CA, USA) in 0.2 ml PBS by intraperitoneal injections at the time of immunization and 48 hr later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)