Citations

Materials:

… PTX was from List Biological Laboratories (Campbell, CA). …

Results:

… LDL activates ERK1/2 and JNK through a PTX-sensitive pathway. Quiescent RMC were serum deprived overnight in the presence or absence of 500 ng/ml PTX before stimulation for 5 min with 50 µg/ml LDL or 5 µm S1P. Basal (NS), LDL-, and S1P-stimulated phosphorylation of ERK1/2 and JNK in whole-cell lysate samples was determined as described. Representative phospho-ERK1/2 (A) and phospho-JNK (B) immunoblots are shown above bar graphs depicting mean sd for four independent experiments. The change in phosphorylation ERK1/2 and JNK are expressed as the percent above the basal level in unstimulated cells not exposed to PTX. *, P < 0.001 vs. unstimulated (NS); and #, P < 0.001 stimulated vs. PTX treated. NS, Not stimulated. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Each mouse received 200 ng pertussis toxin (PTx; List Biological Laboratories, Hornby, ONT, Canada) in 500 µL sterile phosphate-buffered saline (PBS) on the day of immunization and 48 h later. Clinical symptoms …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… PTX was purchased from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PA63 and LF were purchased from List Biological Laboratories (Campbell, CA). …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

ELISA:

… An in-house ELISA was used for measuring anti-diphtheria toxoid (DT). In brief, plates (Medisorp, Thermo Scientific Nunc) were coated with 50l/well DT (1g/ml in PBS) (List Biological Laboratories, Campbell, CA, USA) and blocked with 3% BSA in T-PBS. The sera samples were diluted 1:100 in 3% BSA in T-PBS. Anti-human IgG antibody conjugated with HRP and a substrate was used in the manner described above.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo depletion experiments:

To deplete CD207+ DCs or LCs, 1 g of DT (List Biological Laboratories) was injected i.p. in Lang-DTR mice at day 3 or 15, respectively. DEREG mice were depleted of CD4+Foxp3+ Tregs (8) by i.p. injection of 1 g DT at days 2 or 1 with respect to day 0 of LC transfer.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Autoimmune Encephalomyelitis Model:

EAE was elicited and scored as previously described ³³. Briefly, on day 0 mice received bilateral subcutaneous flank injection of 50 µg of MOG (MEVGWYRSPFSRVVHLYRNGK) peptide in complete Freunds adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (Difco Labs). A dose of 200 ng of Bordetella pertussis toxin (PT; LIST Biological Labs) was injected i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemical experiments:

Retrograde labeling of phrenic motor neurons. To identify phrenic motor neurons, 12 rats were used for retrograde labeling with cholera toxin B (CtB) subunit via intrapleural injection (Mantillaetal.,2009).  Isoflurane anesthesia was induced in a closed chamber and maintained via nose cone (1.5% isoflurane in 100% oxygen). A total of 25 µl of 0.5% CtB subunit (dissolved in 0.9% sterile saline; List Biological Laboratories) was injected into the right and left thoracic cavities (6 mm deep, fifth intercostal space) using a 25 µl Hamilton syringe and a custom-made needle …
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAE with MOG₃₅₅₅ peptide:

EAE was induced as described previously [21], [23], [24] by subcutaneous injection into the mouse flank on day 0 with 300 µg of MOG₃₅₅₅ peptide thoroughly emulsified in complete Freund’s adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (Difco, Detroit, MI). One week later (day 7), the mice were boosted with 300 µg of MOG₃₅₅₅ peptide subcutaneously in the other flank. 500 ng Pertussis toxin (List Biologicals, Campbell, CA) in 200 µl of PBS was injected intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Immature DCs with or without nicotine (NiDCs and MoDCs, respectively) were cultured with 10 ng/ml lipopolysaccharide (LPS; Salmonella minnesota; List Biological Laboratories, INC, Campbell, CA) to induce cytokine and chemokine production. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)