Bacterial Toxin Research Citations

EAE:

… Mice also received a co-injection of 500 µL Bordetella pertussis toxin (List Biological Laboratories, Cedarlane, Hornby, Ontario) on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For the study of pertussis toxin (IAP) pretreatment, sub-confluent monolayers of NRK cells were incubated for 24 h in the presence of IAP (100 ng/ml) (List Biological Lab. Inc., Campbell, Ca) for in vivo ADP ribosylation of G subunits in cells [15]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis:

Mice were immunized with MOG peptide 3555 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in Complete Freunds Adjuvant subcutaneously, in a volume of 0.1 ml/mouse over two sites: the right draining inguinal and axillary lymph nodes. One week later a booster immunization was delivered subcutaneously, over the contralateral draining lymph nodes. Pertussis toxin (500 ng/mouse) (List Biological Laboratories, Inc., Campbell, CA) was injected intraperitoneally on days 0 and 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and monitoring of EAE:

EAE was induced one week after RGC labeling. Mice (n = 5/experiment) were anesthetized and injected subcutaneously at two sites on the back with a total of 200 µg of myelin oligodendrocyte glycoprotein peptide (MOG; Genscript, Piscataway, NJ, USA) emulsified in Complete Freund’s Adjuvant (CFA; Difco, Detroit, MI, USA) containing 2.5 mg/ml mycobacterium tuberculosis (Difco). Control mice (n = 5/experiment) were injected with an equal volume of PBS and CFA. 200 ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.2 ml PBS was injected intraperitoneally on days 0 (day of immunization) and 2 in all mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… When A31 cells were treated with either carboxypeptidase B (CpB, 20 mg/ml; Calbiochem, San Diego, CA, USA) or pertussis toxin (PTX, 100 ng/ml; List Biological Laboratories, Campbell, CA, USA), the cells were incubated with either CpB for 30 min or PTX for 2 h before …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Following immunization, 200 ng of pertussis toxin (List Labs) was injected intraperitoneally (ip) on Day 0, followed by a 400 ng pertussis toxin ip injection on Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

For active EAE, mice were immunized subcutaneously on the back with 200 µg of MOG35-55 (MEVGWYRSPFSRVVHLYRNGK) emulsified in CFA (Difco Lab, Detroit, MI) containing 4 mg/ml Mycobacterium tuberculosis H37Ra (Difco). Two hundred nanogram of pertussis toxin (List Biological Lab, Epsom, England) was given intraperitoneally on days 0 and 2 (p.i.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (List Biological Laboratories) at a dose of 200 µg per mouse in PBS was administered intravenously on the day of immunization and once more 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Fluorescein isothiocyanate-conjugated B subunit of cholera toxin (CTB-FITC) was purchased from List Biologicals (Campbell, CA, USA). …

Confocal laser microscopic examination of the stable transfectants:

To examine the distribution of GM1 ganglioside (GM1) in these transfectants, we used CTB-FITC as specific probe for GM1. The transfectants were cultured on collagen/poly-l-lysine (25:1)-coated coverslips and fixed with 3% paraformaldehyde for 5 min at room temperature and stained with CTB-FITC.

• Product #106 – Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate

Tracer injections:

The rats were deeply anesthetized by intraperitoneal injections of a mixture of ketamine (72 mg/kg) and xylazine (11.2 mg/kg). … The retrograde tracers, cholera toxin subunit (CT; List Biological Laboratories, Campbell, CA; 1% in ddH₂O) … The tracer solution was injected ionotophoretically using positive current pulses (7 s on and 7 s off for 15 min) of 1 A (for FG) and 3 A (for CT). In order to generate more retrograde labeling in the vicinity of the RMTg, some injections of CT into RtGi were done with glass pipettes pulled to tip diameters of 3040 m from which the tracer was expelled by air pressure, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt