Bacterial Toxin Research Citations

Experimental autoimmune encephalomyelitis:

Experimental autoimmune encephalomyelitis was induced in 4-mo-old male B6 or B6.Sle1c2 mice. On day 0, mice received an emulsion of 50 mg myelin oligodendrocyte glycoprotein peptide sequence 3555 and 500 mg Mycobacterium tuberculosis (Difco) in IFA (Sigma-Aldrich) via s.c. injections at the base of the tail. In addition, 500 ng pertussis toxin (List Biologicals) was administered i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (PTX); List Biologicals, USA

 Induction of active EAE:

… Immunization of C57BL/6 mice with ovalbumin was performed as follows: 250 µg OVA- protein and 800 µg H37RA emulsified in CFA were injected subcutaneously, followed by two intraperitoneal doses of 400 ng PTX in PBS at the time of immunization and 48 hr later.

Induction of active EAE in Rag1-/- or Rag1-/-Thy1.21 mice reconstituted with CD4+ and/or CD8+ T cells was induced by 100 µg MOG35-55-peptide or 50 g OVA257-264-peptide and 400 µg H37RA emulsified in CFA, followed by two intraperitoneal doses of 200 ng PTX in PBS at the time of immunization and 48 hr later.
Induction of active EAE in SJL mice was induced by subcutaneous immunization with 250 µg PLP139-151 peptide and 800 µg H37RA emulsified in CFA. PTX (200 ng) was injected intraperitoneally at the time of immunization and 48 hr later when the disease course was studied, but was omitted when lymph node cells were isolated to generate PLP-specific CD4+ T cells in vitro. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Incomplete Freund’s adjuvant and heat-inactivated Mycobacterium tuberculosis (H37Ra) were obtained from Difco Laboratories (Detroit, Michigan), and pertussis toxin was obtained from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

EAE was induced in WTand Kv1.3 KO mice by s.c. injection over two sites in the ank with 100 mg myelin oligodendrocyte glycoprotein (MOG 35 55) peptide (The Johns Hopkins Peptide Synthesis Core Facility) emulsied in CFA (Difco, Detroit, MI). A total of 250 ng pertussis toxin (List Biologicals, Campbell, CA) was administered at the time of immunization and 48 h later by i.p. injection. Mice were monitored daily for clinical signs of EAE and given a clinical score based on the following criteria: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction by active immunization:

For EAE induction, female WTand GILT2/2 mice were immunized by s.c. injections with either 300 mg MOG3555, or 100 mg rMOG proteins in CFA (Difco, Detroit, MI) with 300 mg Mycobacterium tuberculosis on days 0 and 7. Mice received i.p. injections of 500 ng pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and surgical procedures:

Adult male Harlan SpragueDawley rats … were anesthetized with an equal mixture (1 ml/kg body weight) of ketamine (50 mg/ml) and xylazine (10 mg/ml), delivered intramuscularly; supplements of 0.5 ml ketamine were given if required…

Under anesthesia, each animal received a single, stereo-taxically placed iontophoretic co-injection of a mixture of 2.5% PHA-L (#L-1110; Vector, Burlingame, CA) and 0.25% CTB (#104; List Biological, Campbell, CA) prepared in 0.1 M sodium phosphate-buffered saline (pH 7.4). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Recombinant protein reagents:

Recombinant anthrax toxins, rPA, rLF, or rEF (List Biological Laboratories, Campbell, CA) were stored frozen in 1 mg/ml aliquots as recommended by the supplier. …

Protective antigen, lethal factor, and edema factor ELISAs:

Antibody specificity for toxin components was determined using standard ELISAs. High bind plates (Costar 3369) were coated overnight with 1 g/well of rPA, rLF, or rEF. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

… To examine the mucosal immunogenicity of MucoRice-BoHc, … with or without CT (1 µg, List Biological Laboratories, Campbell, CA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE:

EAE was induced by subcutaneous immunization of eight-week-old mice in the flanks with 200µl of an emulsion containing 250µg of MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK) and 400µg of Mycobacterium tuberculosis extract H37 Ra (BD Biosciences, Austin, TX, USA) in incomplete Freunds adjuvant oil. In addition, the animals received intraperitoneal injection of 250ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA). on Day 0 and Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In some experiments, inhibitors were injected intracellularly into the sensory neuron 30 min before the start of an experiment, and were always compared with vehicle injections. EGTA (Calbiochem) and the light chain of botulinum toxin D (List Biological Laboratories) were diluted in the vehicle solution (0.5 M KAc with 10 mM TrisHCl, pH 7.4, and 0.2% Fast Green to visualize the injection).