Bacterial Toxin Research Citations

Neuroanatomical tracer injections and tissue preparation:

… We injected 2 µL of cholera toxin (List Biological Laboratories, Campbell, CA, USA) into the thyroarytenoid muscle at the midportion of the left vocal fold …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Visual stimuli were generated with a hand-held laser pointer or small flashlight. Once the target structure was identified by its response to visual stimulation, a glass micropipette previously filled with native purified cholera toxin B subunit (CTb; List Biological Laboratories, Campbell, CA; 0.5% in phosphate buffer 0.1 M) was lowered into the brain based on the coordinates obtained from the physiological recordings. The tracer was then pressure injected, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

 Vaccine-like treatment of EAE with peptides:

Prior to disease induction, four groups of SJL/J female mice (5-7 weeks) received three s.c. injections of either 100 µl of PBS or 100 µl (100 nmol/injection/day) of PLP-PEG-B7AP, PLP-BPI, and PLP peptides at 11, 8, and 5 days
prior to induction of disease on day 0. … In addition, 200 ng of pertussis toxin
(List Biological Laboratories, Campbell, CA) was delivered intraperitoneally (i.p.) on days 0 and
2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

DC loading with antigen and injection in vivo:

… In some experiments, DC were simultaneously treated with pertussis toxin (List Biological Laboratories, Inc, USA) at 20 ng/ml for 23 h. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

EAE was induced by subcutaneous injection of myelin oligodendrocyte glycoprotein peptide (MOG₃₅₅₅; MEVGWYRSPFSRVVHLYRNGK) injection (9). … Each mouse was injected s.c. with 100 µL MOG₃₅₅₅ emulsion and injected i.p. with 100 µL pertussis toxin (200 ng; List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… LPS (#201 from E.coli 0111:D4) was from List Biological Laboratories. …

Plaque Assays:

For plaquing, confluent monolayers of macrophages in 12-well plates were infected at an MOI of 0.01 with log phase WT Lm similar to the previously described method (Sun et al., 1990). 1 hr later, cells were washed twice with PBS and gent (10 g/ml) was added along with nothing or 4 ng/ml LPS.

• Product #201 – LPS from Escherichia coli O111:B4

Infections and EAE induction:

… EAE was induced 35-40 days post infection by injecting subcutaneously each mouse with 100 µl of emulsified complete Freund’s adjuvant (DIFCO) with 200 µg of MOG 3555 (GenWay biotech, purity >95%) and 400 µg of dessicated Mycobacterium tuberculosis H37ra (DIFCO). Mice also received two i.p. injections with 200 ng of pertussis toxin (List Biologicals) at time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PTx and PTxB were purchased from List Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell isolation:

PBMC were isolated from heparinized venous blood of healthy volunteers by Ficoll-Paque Plus (Ge-Healthcare) density gradient centrifugation as recommended by the manufacturer. Monocytes were isolated from PBMC by MACS with the Monocyte Isolation Kit II (Miltenyi) and their purity was determined by FACS by staining the cells with anti-CD14 and anti-CD45.  … Monocytes were treated with 2 µg/ml Bordetella Pertussis toxin (List Biological Laboratories) washed and subjected to video microscopy.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Evaluation of EAE:

Six- to eight-wk-old C57Bl/6 mice were immunized subcutaneously with 100µl of an emulsion containing 100µg of rMOG and 200µg of Mycobacterium tuberculosis H37Ra (BD Biosciences, San Jose, CA) distributed over three sites on the flank. Mice were injected intraperitoneally with 200ng of pertussis toxin (PTX) (List Biological Laboratories, Cambell, CA) on days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)