Bacterial Toxin Research Citations

2.1. Bacterial Strains, Reagents, Growth Conditions, and Antibiotic Susceptibility

… The presence of Stx2 (List Labs, Campbell, CA, USA) was determined by Western slot-blot analysis of filtered culture supernates with an anti-Stx2 monoclonal antibody [57] that was obtained from Dr. Alison D. O’Brian (Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA). The results of these studies and other characteristics [58,59,60] are presented in Table 1.

• Product #162L – Shiga Toxin 2 from Escherichia coli

… Pertussis toxin (PTX) was purchased from List Biological Laboratories, Inc. (Campbell, California). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For expression analysis, cells were harvested in 0.5 mL of RNAlater™ (Invitrogen™) and stored at -80 °C until analyzed. Escherichia coli LPS (O55:B5; List Biologicals, Campbell, CA, USA) was purchased from List Biologicals (Campbell, CA, USA) and diluted in RPMI culture media …

• Product #203A – LPS from Escherichia coli O55:B5

… complete Freund’s adjuvant (Sigma Co, USA) and Bordetella pertussis toxin (List Biological Lab, USA) were produced for EAE induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3.2. Lipopolysaccharide preparation and administration

LPS doses were prepared in the UAB CRU using an aseptic, no-touch technique. LPS (E. coli Type 0113 Clinical Center Reference Endotoxin) was manufactured by List Biological Laboratories, Inc. The Lot #94332B1 vials containing 1.0 mcg endotoxin were reconstituted to 1.0mcg/1.0 mL, using sterile water. 0.5 mL was drawn and diluted with 9.5 mL of sterile water to produce a 50 ng/mL solution for dosing. Dosages were drawn at 0.4 ng/kg (FM N = 7; HC N = 7) or 0.3 ng/kg (FM N = 5; HC N = 6) body weight. Finally, the dose was diluted in 30 mL sterile saline for administration.

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

Culture supernatant extract anthrax vaccine and its liquid and dry powder formulations preparation and characterization

… Total protein concentrations of the CSE vaccine were measured by bicinchoninic acid assay (BCA, Gene-Star, Beijing, China). An enzyme-linked immunosorbent assay (ELISA, Dakewe, Beijing, China) was used to quantify the PA protein in the CSE vaccine according to a previously method [Citation20]. Serially diluted commercial recombinant PA (rPA, 171E, List Labs, Campbell, CA, USA) was the reference standard. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Pertussis toxin (200 ng/mouse, i.p.; List Biological Laboratories) was injected on the days 0 and 1 relative to immunization. Clinical scores were monitored daily in a blinded manner and recorded as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Human Endotoxemia (IV LPS Administration)

… Following that, it was continuously given at a rate of 150mL/h for the remaining duration of the experiment. Promptly after prehydration, a bodyweight-adjusted bolus dose of 1ng/kg LPS (Escherichia coli-derived, Type O0113, lot no.94332B1; List Biological Laboratories, Campbell, CA, USA) was administered. Blood samples were serially obtained to construct time-concentration curves of circuiting cytokines (Jansen et al., 2022). Temperature and cardiovascular monitoring (blood pressure and heart rate) and sickness symptoms (headache, muscle pain, back pain, nausea, shivers and vomiting were recorded throughout the whole experiment with intervals of 30 minutes. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… The emulsion (200 µL/mouse) was injected subcutaneously at three points on the back of each mouse, and a pertussis toxin (250 ng/mouse) (List Biological Laboratories, 181, CA, USA) solution was intraperitoneally injected at 0 h and 48 h. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… For treatment with pertussis toxin (PTX), the cells were cultured with 100 ng/ml PTX (List Biological Laboratories, Campbell, CA; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)