Bacterial Toxin Research Citations

Reagents

… Bacillus anthracis PA was obtained from List Biological Laboratories. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… A 10× solution of the lethal factor (LF, List Biological Labs) in medium was prepared at 5 µg/mL, and 10 µL of the 10× stock was added to the well to achieve a 0.5 µg/mL …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

2.4. Bone Marrow Derived Mast Cells

Bone marrow-derived mast cells (BM-MCs) were in vitro differentiated into a mucosal-like phenotype as described previously [13]. Briefly, bone marrow cells were collected from femurs and cultured in DMEM with glucose and L-glutamine, supplemented with 10% fetal bovine serum, penicillin/streptomycin, and sodium pyruvate (Biowest, Nuaillé, France), plus stem cell factor, IL-3, IL-9 and TGF-β (Peprotech, Rocky Hill, NJ, USA), for a minimum of 4 weeks and a maximum of 8 weeks before being used. For activation through cross-linking of the IgE receptor, MCs (1 × 106 cells) were sensitized with serum from BALB/c mice orally sensitized with 5 mg of SM, CN, and WP plus 10 μg of cholera toxin (List Biologicals, Campbell, CA, USA) [14]. After washing, MCs were activated with samples in HEPES degranulation buffer. To study the activation through an IgE independent pathway, non-sensitized MCs were also stimulated with samples. β-hexosaminidase was detected by an enzymatic colorimetric assay [15]. The percentage of β-hexosaminidase release was calculated considering the total β-hexosaminidase content.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Multiplex bead assay.

The specificities and avidities of clonal IgG antibodies in culture supernatants were determined in a multiplex bead assay (Luminex Corp.) as described previously (18, 36), with modifications. … Antigens and controls included BSA, … tetanus toxoid from Clostridium tetani (List Biological Laboratories), …

• Product #191 – Tetanus Toxoid from Clostridium tetani

… Pertussis toxin (PTX) (300 ng; List Biological Laboratories) was given intraperitoneally on the day of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Endotoxin derived from Escherichia coli O55:B5, Salmonella minnesota R595 Re and Salmonella typhimurium were obtained from the List Biological Laboratories, Inc. (CA, USA)…

• Product #203A – LPS from Escherichia coli O55:B5
• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #225 – LPS from Salmonella typhimurium

Experimental autoimmune encephalomyelitis (EAE) induction and scoring

On the day of immunization (Day 0), mice were injected subcutaneously over each shoulder with 100 μL of an emulsion containing 0.5 mg/mL MOG35-55 peptide (Bio-Synthesis, 12-668-01) and 1.25 mg/mL heat-killed Mycobacterium tuberculosis (Becton, Dickinson, & Company, 231-141) in Complete Freund’s Adjuvant (Sigma Aldrich, F5881). Mice were intraperitoneally injected with 200 ng of Pertussis toxin (List Biological Laboratories, 180) on days 0 and 2 post-immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… cholera toxin (100 ng/mL; List Biological Laboratories). SUM159 cells were cultured in Ham’s F-12 medium with 5% fetal bovine serum (FBS), 5 µg/mL insulin, and 1 µg/mL hydrocortisone. Hs578T cells were cultured in DMEM supplemented with 10% FBS and 40 µg/mL insulin. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For Tregs depletion, Foxp3-DTR mice were injected intraperitoneally with 1 µg DT (List Biological Laboratories, Inc, USA) 24 h before BCAS, then 0.5 µg every three days …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin (PTX) (180243A1) was purchased from List Biological Laboratories (Campbell, USA) …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer