Bacterial Toxin Research Citations

… Primary antibody Goat anti-CTB List Biological 1:1000 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Pertussis toxin List Biological Laboratories Cat#: 181

EAE induction
Various groups of mice at 8-12 weeks were subcutaneously (s.c.) injected over four sites in the flank with 100 μL of emulsified IFA supplemented with 200 μg of MOG33–55 and 500 μg of heat-killed M.tb as previously described (Ouyang et al., 2019; Xiao et al., 2015). Three hundred ng pertussis toxin (List) per mouse in PBS was injected intraperitoneally (i.p.) at the time of immunization and 48 h later.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Immunoblotting and pull-down assays

Cell lysates and cell culture supernatants were separated by 10% or 15% SDS-polyacrylamide gel electrophoresis under reducing conditions (non-reducing when using the antibody against C5), transferred to a nitrocellulose membrane and blocked with 5% non-fat dry milk in 0.1% Tween 20/TBS solution. The membranes were probed with primary goat antibodies against PA (1:5000, List Biological Laboratories), …

Mouse Model of EAE

Female mice at 6 to 8 wk of age were immunized subcutaneously with 50 to 100 μg of MOG35-55 peptide (MEVGWYRS PFSRVVHLYRNGK; GL Biochem) emulsified in complete Freund’s adjuvant (Sigma) and supplemented with 400 to 800 μg of Mycobacterium tuberculosis H37Ra (Difco). The mice were intraperitoneally injected with pertussis toxin (200 ng/mouse; List Biological Labs) in 100-μL phosphate-buffered saline (PBS) on the same day and 2 d later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cuprizone, EAE, and pertussis toxin injections

Experimental autoimmune encephalomyelitis (EAE) was induced as previously described [56,57,58]. Briefly, emulsion of equal volumes of CFA and 100 µg myelin oligodendrocyte glycoprotein peptide (MOG35–55, MEVGWYRSPFSRVVHLYRNGK) supplemented with M. tuberculosis H37Ra (5 mg/mL, Difco, Detroit, MI) were injected subcutaneously in the scapular region of each mouse. MOG–CFA mixture was emulsified by sonication using a sonic dismembrator (Fisherbrand; FB705). Pertussis toxin (200 ng/mouse, i.p.; List Biological Laboratories) was injected on the days 0 and 2 relative to immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice treatment

CD169-DTR heterozygous or wild-type (WT) male mice were intraperitoneally injected with 40 ng/g of diphtheria toxin (DT) (List Labs, Campbell, CA, USA) diluted in 200 μl DPBS at early stages (a day before and 4 days after tumor injection) and intermediate stages (5 and 10 days after tumor injection) of tumor progression. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

EAE Induction and Assessment

EAE was triggered using a four-point paraspinal subcutaneous injection of an emulsion containing
complete Freund’s adjuvant (CFA) (0.05 mL, Sigma, St Louis, USA), myelin oligodendrocyte glycoprotein
(MOG) 35 − 55 peptide (250 µg, Peptide Biotechnology, Nanjing, China), 0.05 mL of sterilized saline and
Mycobacterium tuberculosis H37Ra (4 mg/mL, Difco Laboratories, Detroit, USA); subsequently, pertussis
toxin (500 ng, List Biological, CA, USA) was given through intraperitoneal injection and again the same amount was injected after 48 h. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction
EAE was induced by injecting 8- to 10-week-old female C57BL/6J mice with 150 mg MOG35–55 peptide (Genemed Synthesis) emulsified in complete Freund’s adjuvant (CFA) (BD Difco) per mouse subcutaneously in the flanks, followed by intraperitoneal administration of 150 ng pertussis toxin (List Biological Laboratories, Inc.) per mouse on days 0 and 2 as previously described [60]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The aLF was ordered from List Biological Laboratories, Inc (Listlabs, US). …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… A3V was injected into one side of the MLd in 19 chicks. Of these, 13 chicks were injected with the mixture of A3V-RSV-mCherry and 0.5% CTB (List Labs, Campbell, CA), and six chicks were injected with A3V-RSV-mCherry …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt