Bacterial Toxin Research Citations

… To induce hair cell damage, DT (List Biological Laboratories, Inc., Campbell, CA) at 25 or 50 ng/g was injected intramuscularly at 7 weeks of age (1-week post-tamoxifen) once a day on two days spaced 44-48 hours apart. We selected a DT dose for each experiment based on periodic tests of DT potency, which varied with each lot and diminished over time during storage at -20°C …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.2. EAE induction and monitoring

EAE mice were induced by subcutaneous immunization with 50 μg of MOG35–55 (MOG001, Chinese Peptide, China) emulsified in CFA (7001, chondrex, U.S.A., 1.5 mg/ml). Intraperitoneal injection of 300 ng Pertussis toxin (181, List Biological Laboratories, U.S.A.) was administered at the time of induction and again 48 h later. Control mice were treated with CFA and Pertussis toxin alone. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Biological Cat# 180 …

EAE induction

… For passive transfer induced EAE, naive CD4 T cells were differentiated under Th17-skewing conditions for 96 h, then IL-17A-GFP positive cells were sorted by using a FACSAria (FACSAria III, BD) and injected intravenously into Rag1−/− mice (1 x 106 cells per mouse). Two days after T cell transfer, recipient mice were immunized with MOG35–55 peptides (300 μg/mouse) emulsified in complete Freund’s adjuvant, containing 5 mg/mL heat-killed Mycobacterium tuberculosis H37Ra, and 200 ng pertussis toxin (PTX, dissolved in 0.1 mL of PBS) was injected intravenously. Mice were then observed daily for disease development. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Immuno-fluorescent visualization of the triple labeling in the INC/DN.

The 30-μm coronal sections were incubated with goat anti-CTB polyclonal antibody (1:200; List Biological Labs, Campbell, CA, USA) and the rabbit anti-PHA-L overnight at 4–8°C. Next day, the sections were incubated in the cocktail of the same conjugated 568 and 488, plus donkey anti-goat conjugated either Alexa Fluor 350 or ByLight 405 to reveal CTB labeled pre-oculomotor neurons; however, the Alexa Fluor 350 was for conventional fluorescent microscopy and the ByLight 405 was for confocal microscopy observations.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.3.9. Immunohistochemistry for VGlut1

Following ex vivo MRI, spinal cords were washed in PBS, cryoprotected in 30% sucrose, frozen in OCT compound and cut in transverse 15 μm sections. Sections were immunostained using; rabbit anti-vGlut1 antibody (1:1000, #135302, Synaptic Systems), Goat-anti-CTB (1:2000, #703, List Biolgicals), Mouse-anti-NeuN (1:500, #MAB377, Millipore). Sections were counterstained with Alexa 546 and Alexa 488 (1:1000, #A10040 and #A10036, Thermofisher) and Dylight 650 (1:500, #ab96938, Abcam). Five sections throughout the L4-L6 segments were selected and traced α-motor neurons (Ctb+/ NeuN+) in the ventral horns were imaged at 400× magnification (LSM 710, Zeiss). Animals with fewer than five traced motor neurons were excluded from analysis.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Peptide-polymer synthesis and nanofiber preparation

For adjuvanted solutions, nucleotide adjuvants (cyclic di-AMP and CpG) were added just before fibrillization; all other adjuvants were added after fibrillization. Adjuvants were included in formulations at the following dosages per mouse: 10 μg of CTB subunit (List Labs, 104), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction and evaluation of EAE

EAE was induced 35 days after γHV68 infection by injecting 100 μl of emulsified Complete Freund’s Adjuvant (DIFCO) containing 200 μg of MOG35–55 (GenScript) and 400 μg of desiccated Mycobacterium tuberculosis H37ra (DIFCO) subcutaneously. Mice also received two doses of 200 ng of pertussis toxin (List Biologicals) intraperitoneally at the time of immunization and 48 hours after induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mgl2-DTR mice were injected intraperitoneally with 1 µg of DT (List Biological Laboratories) 28 days after primary infection (loading dose). Maintenance dosing of 100 ng …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The mucosal adjuvants used were CT (List Biological Laboratories, Campbell, CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Active EAE and adoptive transfer EAE

In active EAE model, … At 0 and 48 h after immunization, the mice were intraperitoneally treated with 500 ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA). …

In another EAE model by adoptive transfer, … At 0 and 48 h after immunization, the mice were intraperitoneally treated with 200 ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)