Bacterial Toxin Research Citations

November 14, 2024

Neurobiology Of Disease

Lika, K;Pant, A;Jefferys, JGR;

Product: Tetanus Toxin from Clostridium tetani

t with sensors (Table 1). TeNT-ECG rats and TeNT-EMG rats (Table 1) both receive unilateral injection into ventral hippocampus 5.4 mm caudal to bregma, 5 mm lateral and 6.8 mm below cortical surface (Paxinos and Watson, 1998). Epileptic rats receive TeNT (25 ng in 250 nl, or 75 ng in 750 nl following a decrease in toxicity due to a freezer malfunction; List Biological Inc., Campbell CA, supplied by Quadratech Diagnostics, Lewes, UK). Batches of toxin can vary in their toxicity, so we routinely assay them (Mellanby et al., 1968; Barkmeier and Loeb, 2009; Sunderam et al., 2009; Wykes et al., 2012). Regulatory considerations mean that we use latency to first seizure after intrahippocampal injection rather than LD50-type tests; our benchmark is a mean of 7 days. In this case we treated the weakened toxin as a new batch. Control rats receive vehicle solution (0.2 % BSA in PBS; Sigma Aldrich). The needle is left in place for 1 min, before injection at 200 nl per minute, and withdrawn after 5 min. PTZ rats recei

November 14, 2024

Cellular & Molecular Immunology

Meng, X;Asadi-Asadabad, S;Cao, S;Song, R;Lin, Z;Safhi, M;Qin, Y;Tcheumi Tactoum, E;Taudte, V;Ekici, A;Mielenz, D;Wirtz, S;Schett, G;Bozec, A;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

lly immunized with 1?mg of OVA (Sigma) or 1?mg of NP-OVA (Biosearch Technologies) plus 10??g of CT (List Biological Laboratories) on days 0, 7 and 14. One week after the final immunization, fecal samples and mononuclear cells from the intestinal lamina propria (iLP) were c

November 13, 2024

Nature Genetics

Guo, W;Zhang, X;Li, L;Shao, P;Liang, C;Zhang, H;Liu, K;Wang, S;Peng, Y;Luo, J;Ju, Y;De Marzo, AM;Yu, C;Chen, L;Zhou, B;Gao, D;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DT (List Labs, cat. no. 150) was dissolved in 1× PBS and administered by intraperitoneal injection at a dose of 50??g?kg?1 dai

November 12, 2024

Fibrosis

Li, Z;Zhu, Z;Wang, J;Zhang, M;Gan, Y;Yan, Y;Wang, L;

Product: Unspecified List Labs LPS

ulture Collection (ATCC), and Equol (purity 99.25%, HY-100583A) was obtained from MCE. Lipopolysaccharide (LPS) from Escherichia coli was purchased from List Labs, and DMSO was obtained from Fangzheng Reagent in Beichen District, Tianjin. Fe

November 7, 2024

Biomedicines

El Samad, A;Jaffal, J;Ibrahim, DR;Schwarz, K;Schmitz, F;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

permeability was enhanced by two intraperitoneal injections of 200 ng pertussis toxin (PTX) from B. pertussis (List Biological Laboratories, Campbell, CA, USA; #181). The first one on the day of immunization (60 min after application of emulsion) and the second one on the subsequent day (16-20 h after immunization). Five independent i

November 7, 2024

Gut Microbes

Taddeo, JR;Wilson, N;Kowal, A;Beld, J;Andres, KS;Tükel, Ç;Tam, VC;

Product: LPS from Salmonella minnesota R595 (Re)

DMSO (naïve control, 1uL/mL media), M2b- Immune complex (IC) of Ova and anti-Ova antibodies (Polysciences, #23744-5, ova: 0.046ug/uL, anti-ova: 0.63 ug/uL) and LPS (from Salmonella Minnesota R595, List Biological Laboratories, 304, 10?ng/mL). Macrophages were then washed with PBS and used for subsequent experiments. Flow cytometry analysis Single-cell suspensions of mesenteric lymph nodes (M ... - DMSO (naïve control, 1uL/mL media), M2b- Immune complex (IC) of Ova and anti-Ova antibodies (Polysciences, #23744-5, ova: 0.046ug/uL, anti-ova: 0.63 ug/uL) and LPS (from Salmonella Minnesota R595, List Biological Laboratories, 304, 10?ng/mL). Macrophages were then washed with PBS and used for subsequent experiments. Flow cytometry analysis Single-cell suspensions of mesenteric lymph nodes (M

November 2, 2024

Inflammation

Karima, S;Khatami, SH;Ehtiati, S;Khoshtinatnikkhouy, S;Kachouei, RA;Jahan-Abad, AJ;Tafakhori, A;Firoozpour, H;Salmani, F;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

heat-killed mycobacterium tuberculosis. Then, this emulsion was injected subcutaneously at the base of the tail of each mouse. The mice also received i.p. injection of pertussis toxin (300 ng in 100 ?l PBS; List Biological Lab, USA) [28, 29]. EAE disease was graded on a 0-5 scale

November 1, 2024

Iscience

Iyer, A;Vaasjo, L;Siththanandan, V;C, R;Thurmon, A;Akumuo, M;Lu, V;Nnebe, C;Nair, R;Galazo, M;Tharin, S;

Product: Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate

Briefly, corticospinal projection neurons were 585 retrogradely labeled at P1 from the cerebral peduncle by injection of cholera toxin B subunit (CTB) 586 from Vibrio cholerae with FITC conjugate (List Biological Laboratories, Campbell, CA) under 587 ultrasound microscopic guidance. At P2, neuron bodies in the motor cortex were isolated by 588 microdissection of deep cortical layers, and dissoci

November 1, 2024

Jacc: Basic To Translational Science

Zhang, D;Wang, X;Zhu, L;Chen, Y;Yang, C;Zhong, Z;Kong, X;Nan, J;Wang, C;Hu, H;Chen, J;Shi, P;Hu, X;Zhu, W;Wang, J;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

e were anesthetized by isoflurane, and mixed AAV solutions (3.2 × 1011 and 2.4 × 1011 vector genomes per mouse, respectively) were injected directly into the myocardium of the left ventricle and apex region. The chest was closed, and the mice were extubated by spontaneous breathing and observed until they were fully awake. To reduce DP macrophages, diphtheria toxin (DT) (List Biological Laboratories) was diluted in PBS and administered to mice IP acutely (25 ng/mg body weight) or chronically (25 ng/mg every other day for 4 weeks). Single-cell RNA sequencing Cell sorting was performed using Aria instrumentation (BD Bioscience). Live single immune cells (4?,6-diamidino-2-phenylindole-CD45+) w

November 1, 2024

Nature Protocols

Tian, S;Qin, Y;Wu, Y;Dong, M;

Product: Anti-Clostridium difficile Toxin B (Chicken lgY)

Mouse monoclonal anti-Actin (Aves Labs, ACT-1010, RRID: AB_2313504) Chicken polyclonal anti-TcdB antibody (List Labs, 754A, RRID: AB_10013220) Chymotrypsin (MedChemExpress, cat. no. HY-108910)

Bacterial Toxin Research Citations

Diaphragm relaxation causes seizure-related apnoeas in chronic and acute seizure models in rats

Metabolic rewiring controlled by HIF-1? tunes IgA-producing B-cell differentiation and intestinal inflammation

JAK/STAT signaling maintains an intermediate cell population during prostate basal cell fate determination

Exploring the Potential Relationship between Gut Microbiome Metabolites and Idiopathic Pulmonary Fibrosis via Network Pharmacology Study

Decreased Expression of the EAAT5 Glutamate Transporter at Photoreceptor Synapses in Early, Pre-Clinical Experimental Autoimmune Encephalomyelitis, a Mouse Model of Multiple Sclerosis

PPAR? exacerbates Salmonella Typhimurium infection by modulating the immunometabolism and macrophage polarization

Acetyl 11-Keto Beta-Boswellic Acid Improves Neurological Functions in a Mouse Model of Multiple Sclerosis

MiR-193b-365 microcluster downstream of Fezf2 coordinates neuron-subtype identity and dendritic morphology in cortical projection neurons

TIMD4hiMHC?hi Macrophages Preserve Heart Function Through Retnla

Design, performance, processing, and validation of a pooled CRISPR perturbation screen for bacterial toxins