Bacterial Toxin Research Citations

August 9, 2024

Microbes And Infection

Belcher, T;Coutte, L;Debrie, AS;Sencio, V;Trottein, F;Locht, C;Cauchi, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

pertussis and/or given 100 ng purified PT (List Laboratories) in 20 ?l of phosphate-buffered saline (PBS) twice 4 weeks apart. To determine the bacterial load at different ;- Bacterial-viral co-infections are frequent, but their reciprocal effects are not well understood. Here, we examined the effect Bordetella pertussis infection and the role of

August 7, 2024

Biorxiv : The Preprint Server For Biology

Willemsen, L;Lee, J;Shinde, P;Soldevila, F;Aoki, M;Orfield, S;Kojima, M;Ricardo, DSA;Sette, A;Peters, B;

Product: Tetanus Toxin from Clostridium tetani

rforming an indirect serological assay with xMAP Microspheres (details described in xMAP Cookbook, Luminex 5th edition). Pertussis, Tetanus, and Diphtheria antigens (PT, PRN, Fim2/3, TT, DT (all from List Biological Laboratories), and FHA (MilliporeSigma)) and as a negative control Ovalbumin (OVA, MilliporeSigma) were coupled to uniquely coded beads (xMAP MagPlex Microspheres, Luminex Corporation) ... rforming an indirect serological assay with xMAP Microspheres (details described in xMAP Cookbook, Luminex 5th edition). Pertussis, Tetanus, and Diphtheria antigens (PT, PRN, Fim2/3, TT, DT (all from List Biological Laboratories), and FHA (MilliporeSigma)) and as a negative control Ovalbumin (OVA, MilliporeSigma) were coupled to uniquely coded beads (xMAP MagPlex Microspheres, Luminex Corporation)

August 5, 2024

Research Square

Yu, R;Lozinski, B;Seifert, A;Ta, K;Zandee, S;Kaushik, D;Park, J;Klement, W;Larouche, S;Tsimikas, S;Witztum, J;Prat, A;Dong, Y;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

ctivated Mycobacterium tuberculosis H37Ra (Sigma-Aldrich), in which 50 µl emulsion was deposited on each side of the tail base. Intraperitoneal injection of pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was performed days 0 and 2 after MOG immunization. Mice were monitored and scored daily on a scale of 0-15. EAE mice during chronic disease (day 45) were euthanized wit ... ctivated Mycobacterium tuberculosis H37Ra (Sigma-Aldrich), in which 50 µl emulsion was deposited on each side of the tail base. Intraperitoneal injection of pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was performed days 0 and 2 after MOG immunization. Mice were monitored and scored daily on a scale of 0-15. EAE mice during chronic disease (day 45) were euthanized wit

August 5, 2024

Journal Of Advanced Research

Zhang, C;Liu, X;Gu, C;Su, Y;Lv, J;Liu, Y;Gao, Y;Chen, H;Xu, N;Xiao, J;Xu, Z;Su, W;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

vol On day 0, we injected mice subcutaneously with 200 ?g of this mixture. After immunization, 0.25 ?g of pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in phosphate-buffered saline (PBS) was injected intraperitoneally on day 0 and day 2

August 4, 2024

Nature Communications

Ma, J;O'Malley, JJ;Kreiker, M;Leng, Y;Khan, I;Kindel, M;Penzo, MA;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

er Scientific) were injected into the TRN (0.5?µL) and PVT (0.6?µL), respectively, and allowed 4 d for retrograde transport. For retrograde tracing of PVT-projecting TRN cells unconjugated CTB (List Labs Product No. 104) was injected into the PVT (0.6?µL). For retrograde labeling of PVT-projecting TRN cells for RNAscope, retrobeads (0.6?µL, LumaFluor, Inc.) were injected into the PVT a

August 1, 2024

Experimental & Molecular Medicine

Kang, YJ;Song, W;Lee, SJ;Choi, SA;Chae, S;Yoon, BR;Kim, HY;Lee, JH;Kim, C;Cho, JY;Kim, HJ;Lee, WW;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

lsified in CFA (BD Biosciences, San Jose, CA), followed by i.p. injection of 250?ng pertussis toxin (PTX) (List Biological Labs, Campbell, CA) 4 and 24?h later. The clinical scores of EAE mice were assessed daily

August 1, 2024

Nature Immunology

Sun, M;Phan, JM;Kieswetter, NS;Huang, H;Yu, KKQ;Smith, MT;Liu, YE;Wang, C;Gupta, S;Obermoser, G;Maecker, HT;Krishnan, A;Suresh, S;Gupta, N;Rieck, M;Acs, P;Ghanizada, M;Chiou, SH;Khatri, P;Boom, WH;Hawn, TR;Stein, CM;Mayanja-Kizza, H;Davis, MM;Seshadri, C;

Product: Enterotoxin Type B from Staphylococcus aureus

for CFP10. Mtb whole-cell lysate from H37Rv was also used to stimulate T cells (BEI Resources). Dimethyl sulfoxide (DMSO) (Sigma-Aldrich) was used as a negative control. Staphylococcus enterotoxin B (List Biological Laboratories) was used as a positive control for the low-exposure cohort. ICS ICS was performed on samples from the low-exposure cohort as we have previously described5. The same ICS ... for CFP10. Mtb whole-cell lysate from H37Rv was also used to stimulate T cells (BEI Resources). Dimethyl sulfoxide (DMSO) (Sigma-Aldrich) was used as a negative control. Staphylococcus enterotoxin B (List Biological Laboratories) was used as a positive control for the low-exposure cohort. ICS ICS was performed on samples from the low-exposure cohort as we have previously described5. The same ICS

August 1, 2024

Nature Immunology

Thakore, PI;Schnell, A;Huang, L;Zhao, M;Hou, Y;Christian, E;Zaghouani, S;Wang, C;Singh, V;Singaraju, A;Krishnan, RK;Kozoriz, D;Ma, S;Sankar, V;Notarbartolo, S;Buenrostro, JD;Sallusto, F;Patsopoulos, NA;Rozenblatt-Rosen, O;Kuchroo, VK;Regev, A;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

H37Ra extract (5?mg?ml-1; Becton Dickinson) in complete Freunds adjuvent (200??l per mouse; Becton Dickinson). Pertussis toxin (100?ng per mouse; List Biological Laboratories) was injected intravenously on days 0 and 2 after immunization. The course of EAE disease was monitored daily, and each mouse was assigned a clinical score according to the following criteria: 0, no sym

July 31, 2024

European Journal Of Immunology

Szymanska, I;Bauernfried, S;Komar, T;Hornung, V;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

20187, MedChem Express), 20 µM Z-VAD-FMK (Peptanova), 10 µM cytarabine (Ara C, Sigma Aldrich), 1 µM MLN4924 (MLN), 1 µg/mL anthrax lethal factor, and 1 µg/mL protective antigen (both List Biological Labs). For poly(I:C) transfection, a transfection mix of poly(I:C) (Invivogen) and Lipofectamine 2000 (Thermo Fisher Scientific) in Opti-MEM (Thermo Fisher Scientific) was prepared according to the manufacturer's instructions. For 96- and 12-well plates, 0.2 and 1.6 µg of poly(I:C) with 0.3 and 2.4 µL of lipofectamine were used, respectively. WT, ?F1L, and rF1L MVA viru

July 31, 2024

Vaccine

Abil, OZ;Liu, S;Yeh, YW;Wu, Y;Sen Chaudhuri, A;Li, NS;Deng, C;Xiang, Z;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

taur Molecular Products; 01-2081A4M; with His-tag) was administered dropwise to the external nares of the mice (10 ?l per nostril). Some mice were immunized with ESAT-6 (5 ?g) admixed to cholera toxin (CT; 1 ?g; List Biological Labs; 100B) as a control adjuvant. Ten days after the last intranasal immunization, various tissues and samples were collected and analyzed, including blood, bronchoalveolar lavage fluid (BALF), nasal lavage, lungs, and spleen. 2.3. Antigen recall responses

Bacterial Toxin Research Citations

Pertussis toxin-dependent and -independent protection by Bordetella pertussis against influenza

Th1 polarization in Bordetella pertussis vaccine responses is maintained through a positive feedback loop

Oxidized phosphatidylcholines induce chronic neurodegeneration partly through IL-1? mediated positive feedback

Histone deacetylases facilitate Th17-cell differentiation and pathogenicity in autoimmune uveitis via CDK6/ID2 axis

Convergent direct and indirect cortical streams shape avoidance decisions in mice via the midline thalamus

Inhibition of BCAT1-mediated cytosolic leucine metabolism regulates Th17 responses via the mTORC1-HIF1? pathway

Specific CD4+ T cell phenotypes associate with bacterial control in people who ‘resist’ infection with Mycobacterium tuberculosis

BACH2 regulates diversification of regulatory and proinflammatory chromatin states in TH17 cells

Vaccinia virus F1Lblocks the ribotoxic stress response to subvert ZAK?-dependent NLRP1 inflammasome activation

A mucosal vaccine formulation against tuberculosis by exploiting the adjuvant activity of S100A4-A damage-associated molecular pattern molecule