Bacterial Toxin Research Citations

July 22, 2024

Biorxiv

Ramamurthy, D;Rodriguez, L;Cen, C;Li, S;Chen, A;Feldman, D;

Product: Botulinum Neurotoxin Type A from Clostridium botulinum

Botox injection to induce paralysis of whisking43,85-87. Both whisker pads were injected with Botox (Botulinum Neurotoxin Type A from Clostridium botulinum, List Labs #130B). A stock solution of 40 ng/?l Botox was prepared with 1mg/ml bovine serum albumin in distilled water. Each whisker pad was injected with 1 ?l of a 10 pg/?l final dilution using a microliter syringe (Hamilton). Whisking stopped within

July 22, 2024

Biorxiv

Flierman, N;Koay, S;van Hoogstraten, W;Ruigrok, T;Roelfsema, P;Badura, A;De Zeeuw, C;

Product: Anti-Cholera Toxin B Subunit (Goat)

peroxidase activity of blood. Next, the sections were blocked in 10% normal horse serum (NHS) in PBS with 0.5% triton, after which they were incubated at room temperature with anti-CTb (goat, 1:15000, List labs) primary antibody (in PBS with 2% NHS, 0.5% triton) for 48-72 hrs and next with biotinylated rabbit anti-goat (1:200, Vector) secondary antibody (also in PBS with 2% NHS and 0.5% triton) for 1.5 hrs. After washing with PBS, the sections were incubated in Avidin-Biotine complex (1:200 Avidine and Biotine, Vector) in PBS and 0.5% triton

July 22, 2024

Microbes And Infection

Mazzone, RJ;Winsor, NJ;Li, LY;Barry, KT;Ranger, A;Goyal, S;Meade, JJ;Bruce, J;Philpott, DJ;Mogridge, J;Girardin, SE;

Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis

1G244 (Millipore Sigma), 5z7 (Sigma-Aldrich), LF (List Labs), Ifn-? recombinant mouse protein (Thermo Fisher), recombinant mouse IL-22 protein (R&D Systems), recombinant murine IL-13 (Peprotech), recombinant m

July 20, 2024

Journal Of Neurochemistry

McDougall, SJ;Ong, ZY;Heller, R;Horton, A;Thek, KK;Choi, EA;McNally, GP;Lawrence, AJ;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

3% isoflurane in air as necessary. The retrograde tracers using a Picospritzer; cholera toxin B subunit (CTb, 20-80?nL, 1% in distilled water; List Biological Laboratories; RRID:AB_10013220) or Red Retrobeads (100?nL, Lumafluor Inc.) were unilaterally or bilaterally injected into the NAc shell: bregma +0.12?mm AP, ±0.07?mm ML, ?0.48?mm DV. Mice were administered meloxica

July 19, 2024

Cellular & Molecular Immunology

Lin, B;Zhou, Y;Huang, Z;Ma, M;Qi, M;Jiang, Z;Li, G;Xu, Y;Yan, J;Wang, D;Wang, X;Jiang, W;Zhou, R;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

l Fast Blue (Sigma, L0294, MCKF3315), DAPI (Sigma, D9542), DNase I (Sigma, DN25), pertussis toxin (List Biological, #181), Freunds Incomplete Adjuvant (Difico, 263910, 8008624), mycobacterium tuberculosis (Difico, 231141, 9347411) and Tamoxifen (MCE, HY-13757A), OptiPrep (Sigma, D1556), Hibernate A (Thermo fisher, A1247501), L-Glutamine (Thermo fisher, 250

July 18, 2024

European Journal Of Immunology

Iijima, N;Hayashi, T;Niino, M;Miyamoto, Y;Oka, M;Ishii, KJ;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

nds were diluted in PBS to 0.01%, 0.5%, or 0.9%, respectively. All emulsions or mixtures were then injected s.c. into the flank skin of mice on days 0 and 7 for the 2×MOG immunization. In addition, pertussis toxin (PTX; 0.2 ?g/mouse; 181; List Biological Laboratories, Inc.) was injected i.v. on days 0, 2, and 7. Alternatively, immunization with PLP1

July 17, 2024

Biorxiv

Goor, I;Altman, E;Arman, I;Haus-Cohen, M;Reiter, Y;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

s with 0.2 ml of an emulsion of 100-200 ?g immunogenic peptide and complete Freunds adjuvant containing 400 ?g of heat-killed Mycobacterium tuberculosis H37RA (Difco, Detroit, MI). In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA) on days 0 and 2 post- immunization (2* 200 ng per mouse). Immunized mice were assessed daily for clinical signs of EAE on a 5-point scale: 0 - Clinically normal, 1 - Decreased tail tone or weak tail only, 2 - Hind limb weakness

July 17, 2024

Infection And Immunity

Bitzer, GJ;Fitzgerald, NA;DeJong, MA;Cunningham, C;Chapman, JA;Boehm, DT;Pyles, GM;Huckaby, AB;Miller, SJ;Dublin, SR;Warden, MD;Barbier, M;Damron, FH;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Plates were coated with 50 µL per well of either whole PT (List Biological Laboratories; cat#180) or an individual subunit of PT synthesized by GenScript Biotech (PTxA, B,

July 16, 2024

Biorxiv

Dürholz, K;Linnerbauer, M;Schmid, E;Danzer, H;Lößlein, L;Amend, L;Ehnes, L;Frech, M;Azizov, V;Schälter, F;Gessner, A;Lucas, S;Lesker, T;Taudte, R;Hofmann, J;Beyer, F;Bootz-Maoz, H;Reich, Y;Romano, H;Mauro, D;Beckervordersandforth, R;Xiang, W;Haghikia, A;Akdis, C;Ciccia, F;Bäuerle, T;Sarter, K;Strowig, T;Yissachar, N;Schett, G;Rothhammer, V;Zaiss, M;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

All mice then received a single intraperitoneal injection of 200 ng 528 pertussis toxin (List Biological Laboratories, #180) at a concentration in 200 ?l of PBS. Mice 529

July 15, 2024

International Journal Of Pharmaceutics

Nesovic, LD;Gonzalez Cruz, PE;Rychener, N;Wilks, LR;Singh Gill, H;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

sh tape re-applied each time) ( Fig. 1 B, C) until treated skin achieved the inflamed and shiny appearance akin to shown in Fig. 1 C (7 tape applications). Next, a foam reservoir (Acrysure Next Gen 1/16? PE Foam, Mactac, OH, USA) was applied on the inflamed skin, while taking care to align the reservoir opening (measuring 1 cm by 1 cm) over the inflamed skin ( Fig. 2 A). A 50 µl of peanut extract (PE) (Greer, Laboratories, Inc., NC, USA) +/- cholera toxin (CT) (List Labs, CA, USA), or PBS alone was pipetted into the reservoir ( Fig. 2 B) and covered with an adhesive film (Silk Double Coated 0.5 mil PET, Mactac, OH, USA) ( Fig. 2 C). Another larger adhe

Bacterial Toxin Research Citations

Reward history cues focal attention in whisker somatosensory cortex

Encoding of cerebellar dentate neuronal activity during visual attention in rhesus macaques

NLRP1B expressed in intestinal epithelial cells is refractory to activation with Val-boro-Pro

Viscerosensory signalling to the nucleus accumbens via the solitary tract nucleus

GPR34 senses demyelination to promote neuroinflammation and pathologies

Tridecylcyclohexane in incomplete Freund’s adjuvant is a critical component in inducing experimental autoimmune diseases

Antigen-Specific modulation of Chronic Experimental Autoimmune Encephalomyelitis in Humanized Mice by TCR-like Antibody Targeting Auto-Reactive T-Cell Epitope

Immunization with an mRNA DTP vaccine protects against pertussis in rats

Gut-specific H3R signaling orchestrates microglia-dependent resolution of peripheral inflammation

Standardizing the skin tape stripping method for sensitization and using it to create a mouse model of peanut allergy