Bacterial Toxin Research Citations

August 23, 2024

Biochemical Pharmacology

Chen, H;Song, J;Zeng, L;Zha, J;Zhu, J;Chen, A;Liu, Y;Dong, Z;Chen, G;

Product: ULTRA PURE LPS from Escherichia coli O111:B4

dose LPS injection method to establish a CKD animal model as described previously [27] . Male 6-8 weeks old C57BL/6J/Sja mice (Hunan Slack King Experimental Animal Company) were maintained under specific pathogen-free conditions. Ultra Pure LPS (Escherichia coli 0111:B4, LIST Biological Laboratories, Campbell, USA) was dissolved in sterile normal saline and injected intraperitoneally (1 mg/kg) every two days to induce chronic endotoxemia for 4 weeks, followed by an injection every two weeks until the 12th week. A subgroup of the mice received a high-salt diet (4 % NaCl in chow plus 1 % in the drinking water) or treatment with

August 22, 2024

Biorxiv : The Preprint Server For Biology

Luebbers, A;Janicot, R;Zhao, J;Philibert, CE;Garcia-Marcos, M;

Product: Pertussis Toxin from B. pertussis (in Glycerol)

irus-containing medium was replaced by fresh medium 6 hr later. In some conditions, the change of media was accompanied by the addition of 0.1 ?g/ml pertussis toxin (List Biological Labs, cat#179A) to wells. Approximately 18-22 hr after the change to fresh medium, cells were washed and gently scraped in room temperature PBS, centrifuged (5 minutes at 550 × g), and resuspended in 750 ?l of BRET buffer. Fifty ?l of cells were added to a white opaque 96-well plate, followed by addition of 50 ?l of BRET buffer and the nanoluciferase substrate Nano-Glo (final dilution 1:200) before measuring luminesc

August 21, 2024

Nature Communications

Glangetas, C;Guillaumin, A;Ladevèze, E;Braine, A;Gauthier, M;Bonamy, L;Doudnikoff, E;Dhellemmes, T;Landry, M;Bézard, E;Caille, S;Taupignon, A;Baufreton, J;Georges, F;

Product: Anti-Cholera Toxin B Subunit (Goat)

2 primary antibody (1/500; Abcam ab18465), or with a rat anti-MBP (1/500, Merckmillipore), a guinea pig anti-NeuN/Fox3 (1/1000, cat 26604, Synaptic system), or with a goat anti-CTb antibody (1/10000, list labs #703), or a rabbit anti-GFP primary antibody (1/1000; Millipore, AB3080), a mouse anti-GAD67 primary antibody (1/500; Millipore MAB5406), a guinea pig anti-PV primary antibody (1/1000, synap

August 20, 2024

Cell Metabolism

Cordeiro, B;Ahn, JJ;Gawde, S;Ucciferri, C;Alvarez-Sanchez, N;Revelo, XS;Stickle, N;Massey, K;Brooks, DG;Guthridge, JM;Pardo, G;Winer, DA;Axtell, RC;Dunn, SE;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Heat-killed Mycobacterium tuberculosis H37Ra Difco Laboratories Difco Laboratories Cat#: DF3114-33-8 Bordetella pertussis toxin List Biologicals List Biologicals Cat#: 181 Luxol fast blue Sigma Sigma Cat#:L0294 Collagenase IV Sigma Sigma Cat#:C5138-500MG Collagenase D Roche Roche Cat#: 11088866001 DNAse I Sigma Sigma Cat#:11284932001 Percoll Fisher Scientific Fisher Scientific Cat#: 45-001-747 FBS Corning Corning Cat#: 35-077-CV EDTA Bioshop

August 16, 2024

Chemrxiv

Ji, J;Kawano, R;

Product:

3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Liss Rhod 80 PE, Avanti Polar Lipids, USA); 1-octanol (SigmaAldrich Co., USA); ?-hemolysin (?HL; Sigma-Aldrich, 81 St. Louis, MO, USA, and List Biological Laboratories, Campbell, CA, USA), Alexa 488 (Thermo Fisher 82 Scientific, USA), Glucose and sucrose (KISHIDA, JAPAN)

August 15, 2024

The Journal Of Clinical Investigation

He, J;Chen, Y;Ding, H;Zhou, JA;Xing, Z;Yang, X;Fan, Q;Zuo, Y;Wang, T;Cheng, J;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

tion. Six- to 8-week-old Vegfb WT and cKO female mice were used in the EAE model. EAE was induced by subcutaneous immunization with MOG35-55 peptides (200 ?g/mouse, catalog T510219, Sangon Biotech) emulsified in complete Freunds adjuvant (catalog F5881, Sigma-Aldrich), followed by administration of pertussis toxin (200 ng/mouse, PTX, List Biological Laboratories Inc.) on days 0 and 2 as described above. Clinical signs of EAE were assessed according to the following score: 0, no signs of disease; 1, loss of tone in the tail; 2, hind limb paresis; 3, hind limb paralysis; 4, tetraplegia; 5, moribund. H&E an

August 13, 2024

Communications Chemistry

Martínez-Carranza, M;kerlová, J;Lee, PG;Zhang, J;Kr?, A;Sirohiwal, A;Burgin, D;Elliott, M;Philippe, J;Donald, S;Hornby, F;Henriksson, L;Masuyer, G;Kaila, VRI;Beard, M;Dong, M;Stenmark, P;

Product: Botulinum Neurotoxin Type A from Clostridium botulinum

% were discounted). The preparations were then washed 3× for 5?min and after a further stabilization period of 20?min, 1?ml of 10x BoNT (final concentration 10?pM for BoNT/A and BoNT/B (both LIST Biological Laboratories, Campbell, USA), and recombinant (r)BoNT/X, and 100 pM for LHN/X, in KHB supplemented with 0.05% (w/v) gelatin type A (Sigma) was added to the tissue bath and electrical s ... % were discounted). The preparations were then washed 3× for 5?min and after a further stabilization period of 20?min, 1?ml of 10x BoNT (final concentration 10?pM for BoNT/A and BoNT/B (both LIST Biological Laboratories, Campbell, USA), and recombinant (r)BoNT/X, and 100 pM for LHN/X, in KHB supplemented with 0.05% (w/v) gelatin type A (Sigma) was added to the tissue bath and electrical s

August 13, 2024

Proceedings Of The National Academy Of Sciences

Diercks, A;Podolskaia, I;Murray, T;Jahn, A;Mai, D;Liu, D;Amon, L;Nakagawa, Y;Shimano, H;Aderem, A;Gold, E;

Product: LIPID A monophosphoryl from Salmonella minnesota R595

ions.Frozen stocks of LPS (List Biological #R595) or PAM3 (Invivogen #vac-pms) were thawed and sonicated for 5 min before dilution in BMDM medium. The diluted stimuli were again sonicated for 5 min before addition to BMDM cultures for a final concentration of 10 ng/mL for LPS and 300 ng/mL for PAM3. CIM cells were stimulated with 100 ng/mL LPS. Unless otherwise indicated, cells were incubated with 25HC (5 ?M) (Sigma #H1015), OSW-1 (0.1 nM) (Cayman Chemicals #30310), itraconazole

August 11, 2024

Journal Of Neuroinflammation

Chumak, T;Jullienne, A;Ek, CJ;Ardalan, M;Svedin, P;Quan, R;Salehi, A;Salari, S;Obenaus, A;Vexler, ZS;Mallard, C;

Product: ULTRA PURE LPS from Escherichia coli O55:B5

ious publication [14]. The day of birth was defined as postnatal day (P) 0. At P9, pups received a single intraperitoneal injection of lipopolysaccharide (LPS from E. coli O55:B5, 1 mg/kg, Cat# 423, List labs) or equivalent volume of saline. The dams remained on the experimental diet until offspring sacrifice (P10-P12). To allocate pups to particular experimental groups (LPS or saline), randomiza

August 10, 2024

Medrxiv : The Preprint Server For Health Sciences

Ismahil, MA;Zhou, G;Gao, M;Bansal, SS;Patel, B;Limdi, N;Xie, M;Antipenko, S;Rokosh, G;Hamid, T;Prabhu, SD;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

netic depletion was performed in CD169DTR/+ mice upon i.p. injection with 10 ?g/kg DT (List Biological, Lot #15043A1) in 100 ?L PBS. For pharmacological depletion, mice were injected i.v. with 4 mg/kg of clodronate-loaded liposomes (Clo-Lip) in 100 ?L suspension solution (Encapsula Nanosciences). This low dose of clo-lip selectively depletes macrophage

Bacterial Toxin Research Citations

Dietary sodium modulates mTORC1-dependent trained immunity in macrophages to accelerate CKD development

Direct detection of endogenous G?i activity in cells with a sensitive conformational biosensor

A population of Insula neurons encodes for social preference only after acute social isolation in mice

Obesity intensifies sex-specific interferon signaling to selectively worsen central nervous system autoimmunity in females

Microfluidic generation of cell-sized liposomes using a budding strategy

Autocrine VEGF-B signaling maintains lipid synthesis and mitochondrial fitness to support T cell immune responses

Activity of botulinum neurotoxin X and its structure when shielded by a non-toxic non-hemagglutinin protein

Oxysterol binding protein regulates the resolution of TLR-induced cytokine production in macrophages

Maternal n-3 enriched diet reprograms the offspring neurovascular transcriptome and blunts inflammation induced by endotoxin in the neonate

Splenic CD169 + Tim4 + Marginal Metallophilic Macrophages Are Essential for Wound Healing After Myocardial Infarction