Citations

Citations

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4784 total record number 156 records this year

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4784 citations found

MicroRNAs -122 and -1247 restrain the pathogenicity of effector CD4+ T cells in autoimmune inflammation

Silva-Santos, B;Cunha, C;Romero, P;Inácio, D;Pais, A;Pelicano, C;Costa, M;Mensurado, S;Gonçalves-Sousa, N;Papotto, P;Neves, D;Sobral, D;Enguita, F;Gomes, A;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • M. tuberculosis in IFA) (Difco Laboratories). On the day of immunization and 2 days after, mice received 200 ng of pertussis toxin (PTx) (List Biological Laboratories) in 100 µl PBS i.v. Mice were weighed daily and scored for EAE clinical signs. In brief, the score system ranged from 0 to 5, with 0.5 increments, being score 0 attributed to an

Transient Seizure Clusters and Epileptiform Activity Following Widespread Bilateral Hippocampal Interneuron Ablation

Dusing, MR;LaSarge, CL;Drake, AW;Westerkamp, GC;McCoy, C;Hetzer, SM;Kraus, KL;Pedapati, EV;Danzer, SC;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • e 1, Experiment 1), followed by a week of baseline 24/7 video-EEG monitoring. Beginning in the fourth week after virus injections, mice were treated with diphtheria toxin or saline. Diphtheria toxin (List Biological Laboratories, #150) was prepared in sterile normal saline at 0.1?mg/ml and stored frozen in aliquots at ?80°C. Diphtheria toxin was diluted to a concentration of 5?ng/µl the da ... e 1, Experiment 1), followed by a week of baseline 24/7 video-EEG monitoring. Beginning in the fourth week after virus injections, mice were treated with diphtheria toxin or saline. Diphtheria toxin (List Biological Laboratories, #150) was prepared in sterile normal saline at 0.1?mg/ml and stored frozen in aliquots at ?80°C. Diphtheria toxin was diluted to a concentration of 5?ng/µl the da

Clinical Relevance of Plasma Prolylcarboxypeptidase Level in Patients with Idiopathic Acute Optic Neuritis

Kim, JH;Shin, DB;Suk, K;Chun, BY;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • n a 100 µL solution containing 50% complete Freund’s adjuvant and 10 mg/mL of a heat-killed H37Ra strain of Mycobacterium tuberculosis (Difco Laboratories, Detroit, MI, USA) [20]. Pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS (200 ng/mouse) was injected intraperitoneally on the day of immunization and again at 48 h post-immunization [20]. The mice were exami ... n a 100 µL solution containing 50% complete Freund’s adjuvant and 10 mg/mL of a heat-killed H37Ra strain of Mycobacterium tuberculosis (Difco Laboratories, Detroit, MI, USA) [20]. Pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS (200 ng/mouse) was injected intraperitoneally on the day of immunization and again at 48 h post-immunization [20]. The mice were exami

Chilean brush-tailed mouse (Octodon degus): a diurnal precocial rodent as a new model to study visual receptive field properties of superior colliculus neurons

Márquez, N;Fernández-Aburto, P;Deichler, A;Perales, I;Letelier, J;Marín, G;Mpodozis, J;Pallas, S;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • ojections to the SC was performed under gaseous anesthesia using isoflurane (4% in medical oxygen at a flow rate of 100 cc/kg/min) complemented with diazepam (5 mg/kg) administrated IP. A glass pipette (tip diameter 15-20 ?m) containing 1% cholera toxin subunit B (CTB, List Biological Laboratories, Campbell, CA) in 0.1 M phosphate buffer was injected intraocularly. After 5-7 days survival, the degus were sedated with gaseous isoflurane, placed in a deep state of anesthesia with ketamine/xylazine (ketamine 40-80 mg/kg, xylazine 5-10 mg/kg), and perfused intracardially by passing 200 ml of warm saline solution (0.9% in 0

CD4+Trm sustain the chronic phase of auto-immune neuroinflammatory disease

Pignata, A;Frieser, D;Hsiao, C;Engelenburg, H;Alis, M;Gonzalez-Fierro, C;Cazaentre, V;Miranda-Capet, R;Dufourd, E;Vermeulen, T;Aida, A;Gisbergen, K;Blanchard, N;Hamann, J;Smolders, J;Liblau, R;Masson, F;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • cton-Dikinson) in incomplete Freund’s adjuvant (Sigma-Aldrich-F5506). Pertussis toxin 200ng/ml (List Labs) was injected intravenously (i.v.) at days 0 and 2 post-immunization. The incidence and the severity of the disease were monitored daily and were evaluated as previously described (56). Isolation of CNS-infiltrating cells and assessment of cytokine production Mice were first anesthetized with an intraperitoneal (i.p.) injection of a solution of

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • onal communication). A mixture of rabies virus (CVS strain, 5.96?×?1010 PFU/ml) and the conventional tracer Cholera toxin B fragment (CTB low salt, end concentration 0.03%) (List Biological Labs, Campbell, CA) was injected in a single dose (2 ?l delivered over 30?min) into MSTl (2 monkeys), rostral MIP (2 monkeys) or LIPv (1 monkey) at midpoint of the rostrocaudal length of the IPS (Figure 1) using a Hamilton syringe driven by a micromanipulator. The injection needle was left in place for more than 30?min after the end of the injection. After recovery from general a

Rapamycin alleviates mitochondrial dysfunction in anti-NMDAR encephalitis mice

Kong, L;Yang, X;Sun, A;Yang, X;Zhao, X;Wang, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

A multi-omics systems vaccinology resource to develop and test computational models of immunity

Shinde, P;Soldevila, F;Reyna, J;Aoki, M;Rasmussen, M;Willemsen, L;Kojima, M;Ha, B;Greenbaum, JA;Overton, JA;Guzman-Orozco, H;Nili, S;Orfield, S;Gygi, JP;da Silva Antunes, R;Sette, A;Grant, B;Olsen, LR;Konstorum, A;Guan, L;Ay, F;Kleinstein, SH;Peters, B;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

A Mucosal Vaccine Formulation Against Tuberculosis by Exploiting the Adjuvant Activity of S100a4A Damage-Associated Molecular Pattern Molecule

Abil, O;Liu, S;Yeh, Y;WU, Y;Sen Chaudhuri, A;Shan Li, N;Deng, C;Xiang, Z;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • 2.2. Immunization and collection of specimens

    Mice were anesthetized with isoflurane before receiving the vaccine preparation for intranasal immunization. A 20-µl phosphate-buffered saline (PBS) solution containing the Mtb antigen ESAT-6 (5 µg; MyBioSource; MBS204541) in the presence or absence of S100A4 (10 µg; Gentaur Molecular Products; 01-2081A4M; with His-tag) was administered dropwise to the external nares of the mice (10 µl per nostril). Some mice were immunized with ESAT-6 (5 µg) admixed to cholera toxin (CT; 1 μg; List Biological Labs; 100B) as a control adjuvant. Ten days after the last intranasal immunization, various tissues and samples were collected and analyzed, including blood, bronchoalveolar lavage fluid (BALF), nasal lavage, lungs, and spleen.

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

A stress sensor, IRE1a, is required for bacterialexotoxin-induced interleukin-1b production in tissue-resident macrophages

Sasaki, I;Fukuda-Ohta, Y;Nakai, C;Wakaki-Nishiyama, N;Okamoto, C;Okuzaki, D;Morita, S;Kaji, S;Furuta, Y;Hemmi, H;Kato, T;Yamamoto, A;Tosuji, E;Saitoh, SI;Tanaka, T;Hoshino, K;Fukuda, S;Miyake, K;Kuroda, E;Ishii, KJ;Iwawaki, T;Furukawa, K;Kaisho, T;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt