Bacterial Toxin Research Citations

2.10 Experimental autoimmune encephalomyelitis

For the induction of EAE, mice were immunized with MP4 antigen as previously described.36 In brief, male C57BL/6J mice at the age of 10 to 16 weeks were injected subcutaneously into both flanks with a total dose of 200 μg MP4 antigen (Alexion Pharmaceuticals, Cheshire, Connecticut), emulsified in complete Freund’s adjuvans (CFA). An intraperitoneal injection of 200 ng pertussis toxin (List Biological Laboratories, Hornby, Ontario, Canada; Cat #181) in PBS was given at the day of immunization and 48 hours later. Mice were sacrificed for analysis during the chronic stage of disease, 43 to 66 days after immunization.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Bordetella pertussis toxin (salt-free) List Biological Laboratories, Inc. Cat # 181

Active EAE
Eight to 12-week-old female and male Cst3−/− and C57BL/6J WT littermates were immunized subcutaneously with 100μg myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) (Stanford Pan Facility) mixed in a 1:1 emulsion with complete Freund’s adjuvant that contained 4mg/ml Mycobacterium Tuberculosis H37Ra (Fisher Scientific). Animals were also administered 400ng of Bordetella Pertussis toxin (PTX) (List Biological Laboratories Inc.) intravenously on days 0 and 2 post-immunization. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.1. Induction of EAE with Myelin Oligodendrocyte Glycoprotein (MOG)35–55 Peptide

Reagents for EAE induction were prepared by diluting MOG35–55 peptide (2 mg/mL; Cat #: AS-60130-5; AnaSpec, Fremont, CA, USA) in UltraPure™ DNase/RNase-Free distilled water (ThermoFisher, Waltham, MA, USA) and emulsifying with equal parts of Complete Freund’s Adjuvant (BD Biosciences, Franklin Lakes, NJ, USA) containing 8 mg/mL Mycobacterium tuberculosis H37RA (Cat #: 231131; BD Biosciences, San Jose, CA, USA) by passage through glass Luer-Lok syringes and a micro-emulsifying needle for 45 min. The emulsion was then transferred to 1 mL Luer-Lok syringes with 27G ½” needles. Pertussis toxin was diluted in UltraPure™ water (2 ng/μL; Cat #: 181; List Biologicals Laboratories, Campbell, CA, USA) and transferred to syringes as described above. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Laboratories Cat# 181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Chemicals, Peptides, and Recombinant Proteins

Pertussis toxin List Biological Laboratories Cat# 181

EAE induction

Naive CD4 T cells from 2D2 mice were differentiated under Th17 conditions in the presence of vehicle or 2.5 ng/ml oligomycin for 5 days, and 1×106 CD4 T cells were transferred into age- and sex- matched Rag1−/− mice. Recipient mice were injected with 200 ng of pertussis toxin (List Biological Laboratories) intraperitoneally on day 0 and day 2.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE induction

To induce EAE disease, complete Freund’s adjuvant, CFA (#F5881, Sigma) containing 400 μg Mycobacterium tuberculosis, Mtb (#DF3114-33-8, Fisher), and 100 μg myelin oligodendrocyte glycoprotein 35–55 peptide (MOG35–55, United Peptides) were subcutaneously administered at 0 and 7 days post-induction (dpi). Pertussis toxin (200 ng/mouse) (#181, List Biological Laboratories, Inc.) was administered on days 0, 2, and 7 dpi. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Biological Laboratories Cat # LB181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Experimental Autoimmune Encephalomyelitis (EAE)

For induced EAE, mice were immunized subcutaneously with 100 g of myelin oligodendrocyte glycoprotein (MOG)35-55 peptide emulsified in complete freund adjuvant (CFA), which was made from IFA(Difco) plus mycobacterium tuberculosis (Difco). Mice were i.p. injected with 200 ng of pertussis toxin (List Biological,#181) on the day of immunization and 2 days later. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Male Col11 GFP mice (2-4 months old) received an injection of pertussis toxin (ip, 500 ng/mouse on days 0 and 2; List Biological Laboratories, Cat.# 181) and an injection of MOG 35-55 peptide…

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

… Pertussis toxin (200 ng per mouse; no. 181, List Biological Laboratories) was administered intravenously 0 and 2 days after induction. Clinical evaluation was performed daily using a five-point scale: 0, no clinical signs; 1, limp tail; …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)