Bacterial Toxin Research Citations

Immunizations

8- to 12-week-old male and female mice were immunized subcutaneously with a 100 μl emulsion of 100 μg α-syn32-46 peptide (A&A peptides; San Diego, CA) dissolved in PBS with Complete Freund Adjuvant (CFA) containing 200 μg M. tuberculosis H37Ra (BD Difco Adjuvant, Fisher Scientific, Cat #DF0638-60-7; Waltham, MA). The day of immunization was designated as day 0. Mice received a boost with the same immunization dose two weeks later. Mice received intravenous injections of 400 ng of B. pertussis toxin (List Biological Laboratories, Cat #181; Campbell, CA) 14- and 16-days post-initial immunization (day 0 and 2 post boost). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… The emulsion (200 µL/mouse) was injected subcutaneously at three points on the back of each mouse, and a pertussis toxin (250 ng/mouse) (List Biological Laboratories, 181, CA, USA) solution was intraperitoneally injected at 0 h and 48 h. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.2. Immunization

Mice were immunized with 200 or 400 μg of AQP4 p201–220 peptide TFA salt (synthesized by Peptide Institute, Inc., Osaka, Japan) emulsified in complete Freund’s adjuvant (CFA), which was made by supplementation of Mycobacterium tuberculosis extract H37Ra (231141; BD, Franklin Lakes, NJ, USA) to incomplete Freund’s adjuvant (263910; BD). A total of 100 μL of CFA was injected intradermally at 15 sites from the base of the tail to the back on Day 0. In addition, mice received 300 or 500 ng pertussis toxin (181; List Biological Laboratories, Campbell, CA, USA) intravenously on Day 0 and intraperitoneally on Day 2. In the experiments establishing AQP4 peptide-immunized mice, we used 400 μg of AQP4 peptide; in the experiments evaluating the effects of IL-6 signal inhibition, we used 200 μg of AQP4 peptide to avoid problems such as death or skin ulcers during the experiment. Control mice were treated with complete Freund’s adjuvant and saline alone.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Biologicals Laboratories (Campbell, CA) Cat# 181 …

Induction of active EAE

Active EAE was induced by subcutaneous (s.c.) injection of MOG35-55 (MOG) peptide (200 µg/mouse) emulsified in complete Freund’s adjuvant (CFA) containing Mycobacterium tuberculosis H37Ra (5 mg/ml) at the flanks of mice. Pertussis toxin (200 ng/mouse) was given intravenously (i.v.) on days 0 and 2. The mice were observed daily for the development of clinical symptoms, and mice were clinically scored.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… All animals were injected with 250 ng pertussis toxin (List Biological Laboratories, PTX181, USA) intraperitoneally on the day of immunization and on day 2 after first …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Labs Cat#181 …

EAE induction
8-week-old, female C57BL/6 mice were s.c. injected with MOG35–55 peptides (300 mg per mouse) emulsified in complete Freund’s adjuvant (CFA) (200 μL per mouse) containing dead Mycobacterium tuberculosis (1 mg per mouse) on day 0. On days 0 and 2, pertussis toxin (400 ng per mouse) was intraperitoneally injected into mice. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE induction and clinical scoring

EAE/optic neuritis was induced with MOG (MOG33-55, GenScript, cat. no.163913-87-9) peptide and PTX (List Biological Laboratories, Campbell, CA, USA, cat. no. 181) in 9-week-old female mice with a procedure modified from the established protocol.29,30,44 Briefly, 225 μg MOG33-55 peptide emulsified with incomplete Freund’s adjuvant (IFA; BD Difco, 263910) and 2.5 mg/mL mycobacterium tuberculosis (BD Difco, 231141) were injected subcutaneously for immunization twice at days 0 and 7, and 500 ng PTX in PBS (2.5 μg/mL) was intraperitoneally injected twice at days 0 and 2 to break down the blood-brain barrier. Mice injected with the same volume of Freund’s adjuvant emulsion without MOG33-55 were used as sham controls. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… adjuvant including heat-killed Mycobacteria tuberculosis (BD Difco, #DF3114-33-8, 200 lg/mouse) on day 0, along with ip injection of pertussis toxin (List Biological 181, …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… pertussis Lyophilized (Salt-Free)List Labs Catalog ##181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Active EAE was induced in 9-12 week-old male and female mice by subcutaneous injection of 150µg of mouse myelin oligodendrocyte glycoprotein peptide (residues 35-55; MOG35-55) (Genemed Synthesis, Inc., San Francisco, USA) emulsified in Complete Freund’s Adjuvant (CFA) (Thermo Fisher Scientific; DF0639606) containing 5 mg/mL heat-inactivated Mycobacterium tuberculosis (Thermo Fisher Scientific; DF3114338). 250 ng of Bordetella pertussis toxin (List Biological Laboratories, Inc. #181) was administered intraperitoneally on the day of immunization and 48 hours later …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)