Bacterial Toxin Research Citations

Induction of Experimental autoimmune encephalomyelitis (EAE)

Wild-type male 10-12 weeks C57BL/6J mice after receiving the combined ICI treatment as described above were induced with active or adoptive transfer experimental autoimmune encephalomyelitis (EAE). Active EAE was induced via SQ injection containing 200 μg MOG35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsified in Complete Freund′s Adjuvant, supplemented with Mycobacterium tuberculosis H37Ra (Hooke Laboratories Cat #EK-0111) as previously reported (6). Briefly, SQ injection was given at dosage of 200 μl emulsion at two sites over the flank region. Additionally, on the day of EAE induction and 48 hours post-induction, 200 ng pertussis toxin (List Biologic Laboratories, Cat. 181) was injected intraperitoneally to each mouse. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Characterization of primary NSCs and C17.2 cells

P3 generation NSCs were seeded into petri dishes t a density of 1×106/ml and cultured overnight. Then, the cells were either treated with different concentrations of BHB for 1 day or pretreated with 500 ng/ml PTX (List Biological, 181), …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Labs Cat# 181 …

α-synuclein immunization booster

To determine immune memory of the α-synuclein immunization, we performed booster injections in two different batches (males and females) which displayed the same results. As customary to standard immunization procedures, booster injections of the α-syn61-75 were repeated halfway through the disease course (i.e., 4 weeks post immunization, 4 wpi) at the same dose and concentration in Incomplete Freud’s Adjuvant (IFA, Sigma-Aldrich) that lacks bacillus. Mice did not receive additional injections of pertussis toxin to prevent hypersensitivity reactions. Sham mice received IFA alone. These mice were additionally bled one week after receiving booster injections.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Establishment of the EAE model

According to a previously established method30, WT C57BL/6 mice were subcutaneously immunized with 200 μg of murine myelin oligodendrocyte glycoprotein (MOG)35–55 peptide (Catalog Number: MOG-001, Chinese Peptide Co., Ltd., Hangzhou, China) in complete Freund’s adjuvant (Catalog Number: 7001, Chondrex Inc., Woodinville, WA, USA). Each mouse was intraperitoneally injected with 400 ng of pertussis toxin (Catalog Number: 181, List Biological Laboratories, Campbell, CA, USA) twice at 0 h and 48 h postimmunization. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Cat#181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Pertussis toxin List Biological Laboratories, Campbell, CA Cat# 181238A1

EAE induction

EAE was induced in female C57BL/6 mice (8–10 weeks of age).38 Mice were subcutaneously immunized with 200 mg of the MOG35–55
peptide in a 100-ml solution (containing 50% complete Freund’s adjuvant with 10 mg/ml of heat-killed M. tuberculosis H37Ra strain).
The MOG35–55 peptide was injected into areas that drained into the axillary and inguinal lymph nodes. Pertussis toxin (200 ng/mouse) was administered intraperitoneally on the day of immunization and again at 48 h later. Animals were examined for disease symptoms
daily. The evaluation of disease severityEAE induction. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Mycobacterium tuberculosis (strain H37Ra; Cat# 231141, Becton, Dickinson and Company, Franklin Lakes, USA), followed by intraperitoneal administration of 400ng of Pertussis toxin (List Biological Laboratories, Cat# 181, Campbell, USA) on day 0 and after 48?h (Bonfiglio et al., 2017). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.3. Study design and active immunization

Action immunization was conducted as previously reported [16]. The peptides were dissolved in saline (2 mg/ml), and then fully
emulsified in an equal volume of complete Freund’s adjuvant containing 1 mg/ml heat-killed Mycobacterium tuberculosis H37Ra
[(CFA), #F5881, Sigma Aldrich, USA]. Mice were randomly assigned to experimental, negative control, or positive control groups.
Each mouse received a subcutaneous injection of emulsion (200 μl containing 200 μg of peptide) at four sites on the back, which was
repeated three times at 2, 4, and 6 weeks (Fig. 1). The GluN1359-378 peptide emulsion was injected as the positive control. For the sham
groups, an equal volume of saline emulsified in CFA was used in place of the peptide. Two hundred nanograms of pertussis toxin (1 ng/μl, #181, List Biological Laboratories, USA) was intraperitoneally administered to each mouse at the time of the last immunization and 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In some experiments, the neurons were pretreated overnight with 500 ng/mL pertussis toxin (Cat. no. 181; List Biologic Laboratory, Campbell, California). No exclusion criteria for low responses to opioid receptor agonists were used, though there was variability in the extent of Ca2+ current inhibition produced by application of the opioid receptor agonists depending on the neuron tested …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Bordetella pertussis toxin (List Biological Laboratories, Inc. #181) the day of immunization and again 2 days later. Motor scores reflecting progressive ascending paralysis were assessed daily using the following scoring system: …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)