Bacterial Toxin Research Citations

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Laboratories Cat# 181…

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… and S1P 1 LysM KO mice immunized with an emulsion containing 200 g Complete Freund’s Adjuvant (CFA), 100 g myelin oligodendrocyte glycoprotein (MOG) peptide 35-55, and 200 ng Bordetella pertussis toxin (lot# 181236A1, List Biological Laboratories …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE induction and clinical assessment:

For immunization, incomplete Freunds adjuvant (IFA) was prepared by mixing paraffin oil (Sigma-Aldrich, St. Louis, USA; Cat # 18512) and mannide monooleate (Sigma-Aldrich; Cat # M8819) at a 9:1 ratio. Complete Freunds adjuvant (CFA) was subsequently obtained by adding 5mg/ml Mycobacterium tuberculosis H37 Ra (Difco Laboratories, Franklin Lakes, NJ, USA; Cat # 231141) to IFA. After emulsifying MP4 (Alexion Pharmaceuticals, Cheshire, CT, USA) in CFA, the mice were immunized subcutaneously into both sides of the flank with a total dose of 200g MP4. Additionally, an intraperitoneal injection of 200ng pertussis toxin (List Biological Laboratories, Hornby, ONT, Canada; Cat # 181) was given at the day of immunization and 48h later. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussin Toxin List Biological Laboratory Cat. #181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… For the induction of MP4-EAE, mice received 200 µg MP4 (Alexion Pharmaceuticals Inc) emulsified in CFA and 200 ng pertussis toxin (List Biological Laboratories, #181, Lot #18123611) in 500 µl sterile phosphate-buffered saline (PBS) on the day of immunization …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin List Biological Cat# 181 …

In vivo suppression assay:

EAE was induced by subcutaneous immunization with 100 ug myelin oligodendrocytepeptide (MOG_35-55; Anaspec) in complete Freunds adjuvant, with 200 ng intraperitoneal pertussis toxin (List Biological) given on days 0 and 2 after immunization. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction of Experimental Autoimmune Encephalomyelitis (EAE) in female C57BL/6 mice:

Experimental autoimmune encephalomyelitis (EAE), a frequently used mouse model of multiple sclerosis (Robinson et al, 2014), was induced in female C57BL/6 mice largely as previously described (Williams et al, 2011). For EAE induction, female C57BL/6 mice older than 10 weeks (body weight between 20 and 25 g) were selected. Mice were injected subcutaneously into the axilla and groin with a total of 200 g of the encephalitogenic MOG₃₅₅₅ peptide of mouse myelin oligodendrocyte glycoprotein (MEVGWYRSPFSRVVHLYRNGK; > 90% purity, synthesized by Dr. Martin Jung, Institute of Medical Biochemistry and Molecular Biology, Medical School Homburg, Saarland University) in sterile water (2 mg/ml) emulsified with an equal volume of complete Freund adjuvant (CFA), consisting of incomplete Freund adjuvant (iCFA, Sigma; #F5506) to which 10 mg/ml inactivated Mycobacterium tuberculosis were added (Fisher Scientific #10218823), as previously described (Williams et al, 2011). 200 ng of pertussis toxin (PTX) from Bordetella pertussis (List Biological Laboratories Inc. #181, via Biotrend, Cologne, Germany) in a volume of 100 l sterile H₂O was injected intraperitoneally on the same day (day 0, 12 h after MOG₃₅₅₅ peptide injection) and also on the subsequent day (day 1, 1620 h after first PTX injection). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

MOG immunization and EAE:

Mice were immunized with 100 mg of myelin oligodendrocyte glycoprotein (MOG)3555 peptide in CFA (Difco) with 500 mg of Mycobacterium tuberculosis (Difco). Mice were i.p. injected 200 ng of pertussis toxin (List Biological,#181) on the day of immunization and 2 days later, as described (Kang et al., 2010). The mice were observed daily for clinical signs and scored as described previously (Shi et al., 2011).

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Pertussis toxin List Biological Laboratories Cat #181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE Induction:

… Pertussis Toxin from Bordetella pertussis List Biological Laboratories 181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)