LPS from Escherichia coli K12 strain LCD25

... To produce foam cells, BMMs were treated with 100 µg/mL of acLDL and 100 ng/mL of LPS (List Biological Labs Inc, Cat. 314), simultaneously, for 24h (RNA) or 48h (protein and other experiments) ...

• Product #314 - LPS from Escherichia coli K12 strain LCD25

Reagents:

Wild-type LPS from E. coli K12 was obtained from List Biological Laboratories (Campbell, Calif). ...

Results:

Wild-type LPS stimulated CD14-independent TNF- and IL-10 secretion

We next set out to investigate whether alteration of the O-Antigen affects interactions between CD14 and the LPS molecule. TNF- and IL-10 secretion was measured in PBMCs pretreated with blocking antibody to CD14 and stimulated with 100 ng/mL LPS, RF5, or DPL. CD14 blockade resulted in significant inhibition of TNF- and IL-10 secretion from cells stimulated with 100 ng/mL wild-type LPS, as has been previously demonstrated ⁽¹⁴⁾, but modest amounts of cytokine secretion did occur. With attenuation or removal of the O-Antigen, secretion of both cytokines in the presence of CD14 antibody decreased to control levels (Fig. 5). DPL, even in 1000 ng/mL concentrations, was unable to stimulate secretion of TNF- in the presence of CD14 blockade (data not shown).

• Product #314 - LPS from Escherichia coli K12 strain LCD25

Reagents:

LPS from the LCD 25 strain of Escherichia coli K12 (Rb-LPS mutant) and LPS of E. coli 0111:B4 (S-LPS) were purchased from List Biological Laboratories (Campbell, CA).

Microtiter Plate LPS Binding Assay:

Immulon-2 96-well microtiter plates were coated with LCD 25 E. coli K 12 LPS or 0111:B4 E. coli LPS (5 g/well), AP-SP-A (positive control for SP-D binding) and myosin (positive control for SP-A binding [550 ng/well]) or nothing (BSA) in 0.1 M Na₂CO₃, 20 mM EDTA, pH 9.6, for 3 h at 37C. After incubation, the LPS solution was removed, and the plates were air-dried overnight. ...

• Product #314 - LPS from Escherichia coli K12 strain LCD25
• Product #201 - Escherichia coli O111:B4