Bacterial Toxin Research Citations

… Pertussis toxin List Biological Laboratores Cat# 181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Animal surgeries

… After conditioning lesion, to label dorsal column tract regenerating axons, 2 μL of 1% cholera toxin-B (List Biologicals, 103B) were injected with a 10-μL Hamilton syringe into the mice’s left sciatic nerve, 4 days before euthanasia. Animals were perfused with 4% paraformaldehyde, and spinal cords were post-fixed for 1 week at 4°C and then cryoprotected in 30% sucrose.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Additionally, 300 ng of Pertussis toxin (PTX, List Biological Laboratorial 181, USA) per mouse was intraperitoneally injected on day 0 and day 2 respectively. The clinical score of EAE …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.2. Induction of active experimental autoimmune encephalomyelitis (EAE)

EAE was induced according to previously described methods [44]. Myelin oligodendrocyte glycoprotein (MOG)35-55 peptide (0.2 mg/mouse) (PRO-371; Prospec) and HKMtb (0.5 mg/mouse) (231141; BD Difco) were mixed 1:1 with PBS, or emulsified 1:1 with adjuvant: IFA (100 μl/mouse) (F5506; SIGMA), Titermax Gold (100 μl/mouse) (G-1; SIGMA), or Montanide™ ISA-51 VG (100 μl/mouse) (Seppic Inc.). Subsequently, the emulsion or mixture was injected s.c. into the flank skin of mice on days 0 and 7 for the 2xMOG immunization. Additionally, pertussis toxin (PTX; 0.2 μg/mouse)(181; List Biological Laboratories, Inc.) was injected i.v. on days 0, 2, and 7.
Alternatively, instead of the MOG35-55 peptide, immunization with the proteolipid protein (PLP)139-151 peptide triggers chronic relapsing-remitting EAE [45]. The PLP139-151 peptide (0.2 mg/mouse) (350349; Abbiotec) and HKMtb (0.5 mg/mouse) were mixed 1:1 with PBS, or emulsified 1:1 with oil emulsion: IFA (100 μl/mouse) or Titermax Gold (100 μl/mouse). The resulting emulsion was injected s.c. into the flank skin of mice on day 0. Furthermore, pertussis toxin (PTX; 0.2 μg/mouse) was injected i.v. on days 0 and 2. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Animals, induction of cholangitis and Baricitinib treatment

Adult male C57BL/6JCrl mice aged 8–10 weeks were purchased from Charles River (Shanghai). Mice were housed under specific pathogen-free conditions at the Zhejiang University. … Murine model of autoimmune cholangitis was established as M. Eric Gershwin reported53, 100 μg 2OA-BSA (in 50 μl PBS) were emulsified with 50 μl of Complete Freund’s Adjuvant (CFA, Sigma-Aldrich) and injected intraperitoneally into 6-week-old female mice. Two days after the initial immunization with 2OA-BSA, mice received 100 ng of pertussis toxin (List Biological Laboratories) in 100 μl PBS by I.P. After two weeks, the mice were re-boosted with 100 μg 2OA-BSA (in 50 μl PBS) emulsified with 50 μl of Incomplete Freund’s Adjuvant (IFA, Sigma-Aldrich) I.P. Mice received, baricitinib (10 mg/kg) in 0.5% methylcellulose, or vehicle only (0.5% methylcellulose), as indicated, once daily per oral gavage 6 weeks after initial 2OA-BSA immunization for 2 weeks. The dosage of baricitinib applied were based on previous works, as exemplified by ref. 54.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diptheria toxin List Biological Laboratories Cat#150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

CD4+T and YFP+ Treg cells adoptive transfer in Experimental autoimmune encephalomyelitis (EAE)

CD4+YFP+ Treg cells from Foxp3cre and Foxp3cre Cxxc1fl/fl mice were enriched using the Mouse CD4 T Cell Isolation Kit (480005; Biolegend) and then sorted using the BD Aria II flow cytometer. Naïve CD4+T cells from 2D2 (MOG35-55-specificTCR transgenic) mice were isolated using the Mouse CD4 Naïve T cell Isolation Kit480039 (480039; Biolegend). … One day after cell transfer, the recipient mice were inoculated subcutaneously (s.c.) with 200μg MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; GenemeSynthesis) emulsified in complete Freund’s adjuvant (CFA) (F5506; Sigma). Intravenous administration of 200 ng of Pertussis toxin (181; List Biological Laboratories) was performed on days 0 and 2 after peptide inoculation. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… and 0.25% CTB (List Biological Labs, Inc., Campbell, California, USA; catalog #104) dissolved in 10 mM sodium phosphate solution. Stereotaxic coordinates targeting the ILA (AP +11.20 mm, ML -0.50 mm and DV -4.40 mm) and ACAd (AP +11.20 mm, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAU model induction and clinical score

EAU was induced in mice by subcutaneous injection of an emulsion containing 2 mg/mL of retinal antigen interphotoreceptor retinoid-binding protein 1-20 (IRBP1-20) (GiL Biochem, Shanghai, China) and complete Freund’s adjuvant (BD Difco, San Jose, CA, USA), with 2.5 mg of Mycobacterium tuberculosis strain H37Ra (BD Difco, San Jose, CA, USA) in a 1:1 volume ratio. In addition, 0.25 μg of pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in PBS was injected on the same day and 2 days after immunization.21,44,45. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice

Rag2−/− (Cat# 033562), Rag2−/−γC−/− (Cat# 014593), Ccl2−/− (Cat# 004434), Foxp3YFP-Cre (Cat# 016959), Scurfy mice (Foxp3−/y, Cat# 006775), Foxp3DTR knock-in mice (Cat# 016958) were purchased from The Jackson Laboratory (Bar Harbour, USA). Ccr2cKO mice were generated by crossing Ccr2loxP (Gempharmatech, Nanjing, China) mice with Foxp3YFP-Cre mice. For Tregs ablation, 6-week-old Foxp3DTR mice were intraperitoneally injected twice a week with 500 ng dose−1 of diphtheria toxin (DT, List Labs, California, USA) for 3 weeks. All mice were housed and maintained under specific pathogen-free (SPF) conditions. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae