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February 22, 2021
Plos One
Pederson, WP;Cyphert-Daly, JM;Tighe, RM;Que, LG;Ledford, JG;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Mouse tracheal epithelial cell harvest and growth conditions
Mice of each genotype (WT, SP-A-/-, SP-A2 223Q, and SP-A2 223K) were euthanized with Urethane and tracheas were removed and placed in Ham’s F-12 media on ice. … The media used to culture the mouse tracheal epithelial cells (MTECs) consisted of Ham’s F-12 media supplemented with 250 ng/ml amphotericin B solution (Hyclone), 20 ng/ml cholera toxin (LIST biological), 104 μg/ml bovine pituitary extract (Lonza), 5 μg/ml insulin (Sigma), 5 μg/ml apo-transferrin (Sigma), 0.1uM dexamethasone (Sigma), 5ng/ml mouse epidermal growth factor (Sigma), 0.01 μM retinol (Sigma), 20 U/ml nystatin (Sigma), 50 μg/ml gentamicin. During the growth and expansion phase of culture, this media was further supplemented with 10% FBS. …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
February 20, 2021
Molecular Immunology
Wu, T;Lei, Y;Jin, S;Zhao, Q;Cheng, W;Xi, Y;Wang, L;Wang, Z;Niu, X;Chen, G;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… emulsified with Freund’s Complete Adjuvant (CFA) containing 5 mg/mL heat-killed Mycobacterium tuberculosis H37RA (Difco Laboratories, Detroit, MI, USA) on day 0. In addition, 200 ng of Pertussis toxin (List Biological…
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 18, 2021
Tuberculosis
Campo, M;Heater, S;Peterson, GJ;Simmons, JD;Skerrett, SJ;Mayanja-Kizza, H;Stein, CM;Boom, WH;Hawn, TR;
Product: ULTRA PURE LPS from Salmonella minnesota R595 (Re)
2.2. Reagents
… Ultrapure Lipopolysaccharide (LPS) was purified from Salmonella minnesota R595 (List Biological …
• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)
February 18, 2021
Nature Neuroscience
Dong, Y;D'Mello, C;Pinsky, W;Lozinski, BM;Kaushik, DK;Ghorbani, S;Moezzi, D;Brown, D;Melo, FC;Zandee, S;Vo, T;Prat, A;Whitehead, SN;Yong, VW;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… A total of 50 µl emulsion was deposited on either side of the tail base. Pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was intraperitoneally injected on days 0 and 2 after MOG immunization …
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
February 12, 2021
Vaccine
McAlister, SM;van den Biggelaar, AHJ;Woodman, TL;Hutton, H;Thornton, RB;Richmond, PC;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
2.5. Multiplex immunoassay
IgG concentrations specific to vaccine antigens PT, PRN, FHA, TT and DT were measured using an in-house multiplex bead-based immunoassay (MIA). IgG specific to FIM 2/3, which is not included in Boostrix, was also measured as a proxy for natural infection. PT, PRN and FHA were kindly provided by GlaxoSmithKline (Belgium). TT was purchased from Sigma-Aldrich. DT and FIM 2/3 were sourced from List Biological Laboratories (USA). A standard curve was generated using a 10-step 3-fold serial dilution of an in-house reference serum. Blanks and two quality control (QC) samples were included on every plate to calculate % critical variance across all assays, which fell between 5.1 and 14.6%. Assay specificity was determined using inhibition and interference assays. No cross reactivity was detected (Supplementary Figs. 1 and 2). …
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #186 – Fimbriae 2/3 from B. pertussis
February 12, 2021
Nature Medicine
Bartsch, YC;Wang, C;Zohar, T;Fischinger, S;Atyeo, C;Burke, JS;Kang, J;Edlow, AG;Fasano, A;Baden, LR;Nilles, EJ;Woolley, AE;Karlson, EW;Hopke, AR;Irimia, D;Fischer, ES;Ryan, ET;Charles, RC;Julg, BD;Lauffenburger, DA;Yonker, LM;Alter, G;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Antigens and biotinylation
… pertussix toxin (List Biological Laboratories), …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 9, 2021
Cell Reports
Omori, S;Tsugita, M;Hoshikawa, Y;Morita, M;Ito, F;Yamaguchi, SI;Xie, Q;Noyori, O;Yamaguchi, T;Takada, A;Saitoh, T;Toyokuni, S;Akiba, H;Nagata, S;Kinoshita, K;Nakayama, M;
Product: LPS from Escherichia coli O55:B5
STAR★methods
KEY RESOURCES TABLE
REAGENT OR RESOURCE Source Identifier
… Ultra-pure LPS List Biological Laboratories Cat# 225 …
ELISA
Mouse peritoneal macrophages were primed with ultra-pure LPS (3 ng/ml; List Biological Lab) for 3 hr and were then stimulated with crystals for 3 hr at 37°C. IL-1β in cell culture supernatants (sup.) was measured by ELISA (R&D Systems).
February 8, 2021
Immunity
Tang, Y;Wang, X;Li, Z;He, Z;Yang, X;Cheng, X;Peng, Y;Xue, Q;Bai, Y;Zhang, R;Zhao, K;Liang, F;Xiao, X;Andersson, U;Wang, H;Billiar, TR;Lu, B;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae List Biological Laboratories, INC. …
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
February 8, 2021
Immunity
Deerhake, ME;Danzaki, K;Inoue, M;Cardakli, ED;Nonaka, T;Aggarwal, N;Barclay, WE;Ji, RR;Shinohara, ML;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… Pertussis Toxin List Biological Technologies Cat# 180 …
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
February 5, 2021
Research Square
Rudensky, A;Hu, W;Wang, Z;Feng, Y;Schizas, M;Hoyos, B;Veeken, J;Verter, J;Puerto, R;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
4-hydroxytamoxifen, diphtheria toxin, and tamoxifen treatment
… Diphtheria toxin (DT, List Biological Laboratories, 150) was dissolved in PBS. 1µg (first dose) or 613 250 ng (subsequent doses) was injected intraperitoneally for each mouse. …
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
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