Citations

Induction and Evaluation of EAE

… On days 0 and 2, immunized mice received an intraperitoneal injection of 200 ng pertussis toxin (PTX, Cat: 181, Lot: 181238A1, List Biological Labs, CA, USA). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Reagents

… LPS from E. coli O111:B4 was purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #201 – LPS from Escherichia coli O111:B4

… On days 0 and 2, 100 ng/mouse pertussis toxin (List Biological Laboratories) was given by ip injection. Mice were monitored and weighed daily until day 28 of the experiment. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… overnight at 4°C on a shaking platform in either polyclonal rabbit anti-FG primary antibody (1:50,000; Cat# Fluoro-gold, RRID:AB_2314408; Fluorochrome) or polyclonal goat anti-CTB primary antibody (1:10,000; Cat# 703, RRID:AB_10013220; List Biological Laboratories …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Mice were then injected i.p. with 60 ng of pertussis toxin (List Biological Laboratories, 179A) in PBS at 2 and 24 h after emulsion injection …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. Antigens

Vibrio cholerae toxin (CT; lot 10154B5A) and Staphylococcus aureus enterotoxin type B (SEB; lot 1223B1) were from List Biological Labs (Campbell CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #122 – Enterotoxin Type B from Staphylococcus aureus

… Diptheria toxin List Biological Laboratories Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cell Stimulation and Total RNA Extraction

Cells grew in 6-well plates at 37°C with 5% CO2 until they were 80% confluent. The cells were stimulated in a serum-free medium for 6 h with LPS 100 ng/mL (Escherichia coli K12, List Biological Laboratories, Campbell, CA, USA), …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

2.2. Induction of EAN and evaluation of clinical score

P0180–199 peptide (SSKRGRQTPVLYAMLDHSRS, > 98% purity) was synthesized by Ontores Biotech (Hangzhou, China). Pertussis toxin (PTX) was purchased from List Biological Laboratories (CA, USA). Freund’s incomplete adjuvant and Heat killed Mycobacterium tuberculosis (strain H37RA) were purchased from Becton Dickinson (BD) Difco (MI, USA). In brief, mice were anesthetized with isoflurane each time before immunization. PTX solution of 1.6 μg/mL 250 μL, 1.2 μg/mL 250 μL, and 1.2 μg/mL 250 μL were given respectively on Day -1, 1, 3 intraperitoneally. P0180–199 peptide of 2 mg/mL 25 μL was emulsified in Freud’s complete adjuvant (20 mg/mL solution of Mycobacterium tuberculosis in 25 μL Freund’s incomplete adjuvant). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Three hours after nucleofection, cells were stimulated with 1 µg/mL of TcdA (List Biological Laboratories, Campbell, CA, USA) …

• Product #152C – Toxin A from Clostridium difficile