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March 2, 2021
Proceedings Of The National Academy Of Sciences Of The United States Of America
Wang, MY;Dean, ED;Quittner-Strom, E;Zhu, Y;Chowdhury, KH;Zhang, Z;Zhao, S;Li, N;Ye, R;Lee, Y;Zhang, Y;Chen, S;Yu, X;Leonard, DC;Poffenberger, G;Von Deylen, A;McCorkle, SK;Schlegel, A;Sloop, KW;Efanov, AM;Gimeno, RE;Scherer, PE;Powers, AC;Unger, RH;Holland, WL;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
Diabetes Induction
Homozygous PANIC-ATTAC mice were administered a single dose of AP20187 homodimerizer (0.08125 mg/kg) prepared according to the manufacturer’s instructions (17). Female NOD mice were surveyed twice-weekly by glucometer, until spontaneous diabetes was diagnosed by glucose >250 mg/dL for two consecutive measurements. DT (List Biological Laboratories; 150) was administered to NSG-DTR mice in a single, 300-μL intraperitoneal injection of 5.0 ng total DT. Control NSG-DTR mice (phosphate-buffered saline; PBS) were treated with an equal volume of 1× PBS (Sigma-Aldrich). All animals in a cohort (with human islets from the same human donor) were injected with DT or PBS on the same day (29).
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
March 1, 2021
Diagnostic Microbiology And Infectious Disease
Scobie, H;Khetsuriani, N;Efstratiou, A;Priest, J;
Product: Diphtheria Toxoid, from Corynebacterium diphtheriae
… Diphtheria toxoid (60 µg) (List Biological Laboratories, Campbell, CA) and a negative control of recombinant Schistosoma mansoni glutathione-S-transferase (GST) (15 µg) (Moss et al., 2004) were each coupled to 1.25 × 10 7 beads in a 1 ml volume of buffer containing …
• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae
March 1, 2021
Advanced Healthcare Materials
Kelly, SH;Opolot, EE;Wu, Y;Cossette, B;Varadhan, AK;Collier, JH;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
… Adjuvanted formulations contained cholera toxin B (List Biological, cat #104) …
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
March 1, 2021
Immunity
Wang, Y;Fu, Z;Li, X;Liang, Y;Pei, S;Hao, S;Zhu, Q;Yu, T;Pei, Y;Yuan, J;Ye, J;Fu, J;Xu, J;Hong, J;Yang, R;Hou, H;Huang, X;Peng, C;Zheng, M;Xiao, Y;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… Pertussis toxin List Biological Laboratories Cat# 180…
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
March 1, 2021
Shock
Wong, E;Xu, F;Joffre, J;Nguyen, N;Wilhelmsen, K;Hellman, J;
Product: Unspecified List Labs LPS
… Then ultrapure LPS (0.01 µg/mL, List Laboratories, Campbell, CA) was added to the wells in the continued presence or absence of ERK1/2 inhibitor (1 µM) …
Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/
March 1, 2021
FASEB Journal : Official Publication Of The Federation Of American Societies For Experimental Biology
Pouzol, L;Baumlin, N;Sassi, A;Tunis, M;Marrie, J;Vezzali, E;Farine, H;Mentzel, U;Martinic, MM;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
2.2 Myelin oligodendrocyte glycoprotein (MOG)‐induced EAE model
Under anesthesia with isoflurane, female C57BL/6 mice were immunized in the rear flanks by subcutaneous injection (s.c.) of 150 μg MOG35‐55 peptide (Biotrend Chemicals, Zurich, Switzerland) emulsified 1:1 in Complete Freund’s Adjuvant (CFA) (Chondrex, Redmond, WA, USA) containing 4 mg/mL Mycobacterium tuberculosis. On days 0 and 2 postimmunization, mice were injected intraperitoneally (i.p.) with 400 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA). Vehicle or ACT‐1004‐1239 were given from day 0 after immunization.
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 26, 2021
Frontiers In Immunology
Doroshenko, E;Drohomyrecky, P;Gower, A;Whetstone, H;Cahill, L;Ganguly, M;Spring, S;Yi, T;Sled, J;Dunn, S;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
EAE Induction and Clinical Scoring
EAE was induced after a minimum of 30 days post TAM administration by subcutaneous immunization at two sites of the chest with an emulsion that contained 100 μg MOG p35-55 (Genemed Synthesis) and Complete Freund’s Adjuvant containing 200 μg of heat-killed Mycobacterium tuberculosis (H37RA, Difco Laboratories). Mice were also injected with Bordetella pertussis toxin (PTX) (List Biologicals, dose used was lot-dependent, but varied between 100 and 250 ng) on days 0 and 2 following immunization. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 25, 2021
Frontiers In Neural Circuits
Cruz, A;Kim, T;Smith, R;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
Stereotaxic Surgery
… For conventional retrograde tracing, rats were given unilateral microinjections of 100 nl of 2% Fluoro-Gold (FG, Fluorochrome, Denver, CO, USA) into unilateral NAc core (AP +1.8, ML +2.6, DV –7.3 from bregma, 6°) and 100 nl of 0.2% cholera toxin subunit b (CTB, List Biological Laboratories, Campbell, CA, USA) into RMTg (AP –7.2, ML +1.9, DV –7.6 from dura, 10°). FG was used only in NAc because it tended to cause toxicity in RMTg, evident by circling of animals after unilateral injection. Rats were sacrificed at least 1 week after injections. …
Tissue Processing
… For FG and CTB tracing, free-floating sections were incubated overnight in goat anti-CTB (1:50 K, List Biological Labs Cat# 703, RRID: AB_10013220) in PBS with 0.25% Triton X-100 …
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)
February 24, 2021
Nature
Lim, SA;Wei, J;Nguyen, TM;Shi, H;Su, W;Palacios, G;Dhungana, Y;Chapman, NM;Long, L;Saravia, J;Vogel, P;Chi, H;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… Mice were immunized subcutaneously at four injection sites with a total of 200 µl of emulsified incomplete Freund’s adjuvant supplemented with … and received intraperitoneal injections of 200 ng pertussis toxin (PTX; List Biological Laboratories) at the time of immunization and 48 h later …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 23, 2021
JCI Insight
Yu, KK;Fischinger, S;Smith, MT;Atyeo, C;Cizmeci, D;Wolf, CR;Layton, ED;Logue, JK;Aguilar, MS;Shuey, K;Loos, C;Yu, J;Franko, NM;Choi, RY;Wald, A;Barouch, DH;Koelle, DM;Lauffenburger, D;Chu, HY;Alter, G;Seshadri, C;
Product: Enterotoxin Type B from Staphylococcus aureus
Flow cytometry of T cells
… For ICS, we stimulated cells with overlapping peptide pools (15 mers overlapping by 11 amino acids) targeting the S1 or S2 domains of spike glycoprotein, N, or E proteins (JPT Peptide Technologies). The S1 pool spans the N-terminal amino acid residues (1–643 amino acids, 158 peptides) of spike glycoprotein, while the S2 pool spans the C-terminal amino acid residues (633–1273 amino acids, 157 peptides). Each peptide pool was reconstituted with 40 or 50 μL of pure DMSO (Sigma-Aldrich) and then diluted with PBS for a final concentration of 100 μg/mL in 16% DMSO/84% PBS or 20% DMSO/80% PBS. PBMC were plated at a density of up to 1 × 106 cells/well in a 96-well U-bottom plate and stimulated with 1 μg/mL of each peptide in the pool, or 0.25 μg/mL Staphylococcal Enterotoxin Type B (SEB) (List Biological Laboratories Inc.) …