Citations

Preparation of C. difficile toxins

Purified C. difficile TcdA (#153) and TcdB (#154A), as well as the components of CDT, CDTa (#157A), and CDTb (#158A), were procured from List Biological Laboratories, Inc. …

Author provided incorrect List Labs product #’s; refer to the following corrected product #’s:

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile
• Product #753A – Anti-Clostridium difficile Toxin A (Chicken lgY)
• Product #754A – Anti-Clostridium difficile Toxin B (Chicken lgY)

… Additional intratracheal treatments included lipopolysaccharide (LPS, List Labs), …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Seven days before perfusion cholera toxin B-fragment (CTB; 1%, 10µl; List Biologicals) was bilaterally injected into the extensor carpi radialis muscle, using a Hamilton syringe, to anterogradely label PA axons in the spinal gray matter as well as retrogradely label motoneurons in the ventral horn. CTB was tinted with Evans Blue …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.5 Experimental animals and the establishment ofthe EAE model

… Each mouse was injected with antigen emulsion to induce EAE in the dorsal lumbosacral bulking region. The day of immunization was recorded as day 0 post-immunization (p.i.). Each mouse was intraperitoneally injected with 300ng pertussis toxin (PTX, List Labs, USA) on day 0 and day 2 p.i. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis Toxin List Biologicals Cat#179A …

G-protein live-cell BRET measurements

…For endpoint measurements shown in Figure 1—figure supplement 1, data is presented as raw BRET ratios (535 nm luminescence/460 nm luminescence) of unstimulated cells. For Figure 2—figure supplement 1, PTX treatments consisted of overnight incubations with 0.2 µg/ml of the toxin. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… assay (ELISA) protocol (15) with a few modifications. Briefly, 96-well enzyme immunoassay (EIA) plates were coated with 100ml of purified C. difficile TcdA (List Laboratories; catalog no. 152C) at 1mg/ml in 0.05 M sodium bicarbonate …

• Product #152C – Toxin A from Clostridium difficile

… Adherent cells were exposed to C. difficile toxin B (List labs, Campbell CA) …

• Product #155 – Toxin B from Clostridium difficile

…For cholera toxin B subunit (CTB, List Biological Laboratories) treatment, … Pam3CSK4 (InvivoGen) primed cells were stimulated with 20 mg/ml CTB plus …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE model establishment:

… An intraperitoneal injection of 200 ng of pertussis toxin was administered on day 2. The mice were examined daily for clinical signs of disease and evaluated as previously described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)