Citations

… Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae was purchased from List Biological Laboratories, INC. …

Cell culture

… The concentration of agonists or stimuli were used as below: For NLRP3 inflammasome activation: peritoneal macrophages were primed with LPS (100 ng/mL) for 3 h followed by stimulation with 5 mM ATP (1 h); 10 μM Nigericin (1 h); 200 μg/mL MSU (6 h). For non-canonical NLRP3 inflammasome activation, macrophages were primed for 4 h with Pam3CSK4 (100 ng/mL), then treated with LPS (2 μg/mL) combined with CTB (5 μg/mL) (CTB + LPS), or LPS (2 μg/mL) transfected with FuGENE HD (0.25% v/v) for 16 h. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis Toxins List Biological Laboratories, INC. Cat#180 (50 ug) …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… pertussis toxin List Biological Laboratories Cat. # 179A …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Multiple primary antibodies were used in combination, … goat-anti-CTB (1:10,000; RRID: AB_10013220; List Biological Laboratories), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Mice

Mice used included C57BL/6J (Charles River, #027), Tlr4 KO (Jackson Laboratory, #029015), Tlr4 floxed (Jackson Laboratory, #024872), iDTR and Mcpt5-Cre + (provided by Axel Roers, University of Technology, Dresden, Germany). All mice were used between 6 and 10 weeks. For mast cell depletion in Mcpt5-Cre + x iDTR mice, 8-week old mice were given 200 ng Diphtheria Toxin (DT, Unnicked, from Corynebacterium diphtheriae, List Biological Laboratories) per mouse intravenously once a day for 5 consecutive days prior to intradermal ear immunization. Mcpt5-Cre (- x iDTR mice were used as littermate controls. Control mice were also injected with DT to control for off target effects caused by DT. To generate mast cell-specific TLR4-deficient mice, Mcpt5-Cre + mice were crossed with Tlr4-floxed mice. Cre-non-expressing animals were used as littermate controls. ) per mouse intravenously once a day for 5 consecutive days prior to intradermal ear immunization. Mcpt5-Cre (- x iDTR mice were used as littermate controls. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Studies with Primary Tracheal Basal Epithelial Cells

Primary mouse tracheal basal epithelial cells (MTECs) were isolated from tracheas of either young (2 mo) or old (13–16 mo) wild-type (WT) C57BL/6J mice (Jackson Laboratories, Bar Harbor, ME) using overnight incubation with 0.1% protease 14 (Sigma-Aldrich, St. Louis, MO), as previously described (38, 39). Briefly, dissociated MTECs were propagated on a rat tail collagen I gel (BD Biosciences, San Jose, CA) in DMEM/F12 media (Invitrogen, Grand Island, NY) supplemented with 20 ng/mL cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE model studies

The EAE model was induced in both wild type and hCXCR2 knock-in mice using synthetic myelin oligodendrocyte glycoprotein peptide (MOG35-55, MEVGWYRSPFS RVVHLYRNGK; GenScript). Briefly, mice were s.c. injected with an emulsion of 100 μg MOG35-55 peptide mixed with complete Freud’s adjuvant (IFA, #vac-ifa, InvivoGen) containing 400 μg Mycobacterium tuberculosis H37 Ra (#231141, Difco). A total of 400 ng pertussis toxin (#180, List Biological) were i.p. injected on the same day of MOG35-55 immunization and a second time at 48 h later. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Light microscopy immunofluorescence histochemistry

For CTb- and FG-injected rats, sections containing Vpdm plane were selected for immunofluorescence staining of neuronal nuclei (NeuN)/CTb/FG and NeuN/CTb/VGLUT1, respectively. The experimental procedures were as follows: (i) rabbit anti-VGLUT1 IgG (1:500; Synaptic System)/rabbit anti-FG IgG, mouse anti-NeuN IgG (1:1000; Millipore), and goat anti-CTb IgG (1:2000; List Biological) were used to incubate slices at room temperature overnight, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents—Protective antigen (PA) and lethal factor (LF) were purchased from the List Biological Laboratories (Campbell, CA, USA). …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… On days 0 and 2, 200 ng pertussis toxin (List Labs, Campbell) per mouse was given by intraperitoneal injection. Mice were monitored and weighed daily until day 28 of the experiment. The following clinical scores were used: 1, limp tail; 2, hind-limb paresis; 3, hind-limb paralysis; 4, tetraplegia; and 5, moribund …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)