Bacterial Toxin Research Citations

2.2 Regional Iba-1 responses in an in vivo ferret model of inflammation-sensitized perinatal brain injury

On the morning of P17, ferret kits were removed from the nest, weighed, and randomized to either the nonsurgical control or the hypoxic-ischemic-hyperoxic (HIH)-exposed group, as previously described.4 Briefly, controls and HIH-exposed ferrets received 3 μl/g saline vehicle or 3 mg/kg lipopolysaccharide (LPS, ultrapure from E. coli 055:B5, List Biological, CA) intraperitoneally, respectively. Animals that received LPS underwent a bilateral carotid ligation under isoflurane anesthesia (3%–5%) plus buprenorphine (0.05 mg/kg) analgesia. …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

CHO Cell Cluster Response—Chinese Hamster Ovary (CHO) K-1 cells were obtained from ATCC and maintained in F12 + 10% FBS. For CHO cell-PT assays, CHO cells were plated in a 96-well tissue culture plate at 2.5 × 104/mL in F12 + 1% heat-inactivated (HI) FBS and grown overnight at 37 °C, 5% CO2. PT preparations purified in the Hewlett lab and obtained from List Laboratories were comparable in activity. PT was added at indicated concentrations to duplicate wells and the mixture incubated for 24 h at 37 °C, 5% CO2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

EAE was induced in 8–10-week-old female C57Bl/6 mice (Jackson Laboratories, Bar Harbor, ME). Mice received a subcutaneous immunization at each hind flank of 50 μg of myelin oligodendrocyte glycoprotein (MOG35–55) in complete Freund’s adjuvant (CFA; Fisher Scientific, Waltham, MA) supplemented with myobacterium tuberculosis on day 0. Pertussis toxin (List Biological Laboratories, Hornby, ON, Canada) was administered via intraperitoneal injection at a dose of 300 ng/200 μL on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Administration of Drugs

DT (15046A1, List Biologicals Laboratories) was used as previously described.26 Conditional ablation of Tregs in DEREG mice was achieved by intraperitoneal injection of DT (50 µg/kg body weight) 2 days before the MCAO model. Subsequently, an intraperitoneal dose of DT (10 µg/kg body weight) was administered every other day until day 3 after MCAO. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… 20 ng/ml cholera toxin (List Biological Laboratories; Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… PTX was purchased from List Biological Laboratories …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Microinjection of CTB (low salt formulation; List Biological Labs, Campbell, CA, USA) was performed into the wall of selected regions of the LUT under isoflurane anesthesia (3% in 125 oxygen for induction, 1-2% for maintenance), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis Toxin (PTX) from List Biological Laboratories (Campbell, CA); …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin (CT) was purchased from List Biological Laboratories (Campbell, CA, USA). Mice were subjected to weekly intragastric (i.g.) PN sensitization from week 0 through week 5 with ground peanut (10 mg) plus CT (20 µg) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAU model induction and EAU clinical score

For active induction of EAU, mice were injected subcutaneously with an emulsion consisted of 2 mg/mL of retinal antigen interphotoreceptor retinoid-binding protein 1–20 (IRBP1–20) (GPTHLFQPSLVLDMAKVLLD, GiL Biochem, Shanghai, China) and complete Freund’s adjuvant (BD Difco, San Jose, CA, USA) containing 2.5 mg of Mycobacterium tuberculosis strain H37Ra (BD Difco, San Jose, CA, USA) in a 1:1 volume ratio. Additionally, 0.25 µg pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in PBS was injected on the same day and 2 days after immunization (Chan et al., 1990; Agarwal et al., 2012; Chen and Caspi, 2019b). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)