Citations

Mice

C57BL/6 mice were either home-bred or were purchased from SamTako Ltd (South Korea). B6.SJL, Pmel Tg , Il17a Cre mice were purchased from Jackson Laboratory. Foxp3 DTR mice were kindly provided by Dr. Sin-Hyeog Im (POSTECH) with the permission of Dr. Alexander Rudensky (Memorial Sloan Kettering Cancer Center).23 R26 DTA mice and R26 YFP mice were previously described.24 25 Il17a Cre ×R26 YFP , Il17a Cre ×R26 DTA, and Il17a Cre ×R26 DTA/YFP mouse systems were created by breeding the relevant strains. CCSP Cre ×K-ras G12D mice have been described.7 Male or female mice of 8–12 weeks were used, and each experiment was conducted using age-matched/sex-matched groups. For DTR-mediated depletion experiments, 500 µg of diphtheria toxin (List Biological Laboratories) was intraperitoneally treated every other day after tumor injection in Foxp3 DTR mouse systems. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Induction and assessment of EAE

EAE was induced by subcutaneous injection with 200 μg of mouse MOG35–55 peptide (Prespec) emulsified in complete Freund’s adjuvant containing heat-killed Mycobacterium tuberculosis H37RA (BD Difco) on day 0. Pertussis toxin (200 ng; List Biological Laboratories) was intravenously injected on the day of immunization and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★Methods
Key resources table

Lipopolysaccharide from Salmonella Minnesota List Labs Cat#R595

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Pertussis toxin (150 ng per mouse, list labs) was given i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

MATERIALS AND METHODS.

4.1 Reagent.

… Purified LPS was purchased from List Biological Lab. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Cell cultures

… Established cancer cell lines were cultured according to ATCC recommendations. DT22 cells and BM-MSCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10%fetal bovine serum (FBS). MCF10A cells were cultured in DMEM/Ham’s F12 medium supplemented with 5% horse serum (Gibco BRL, Waltham, MA), insulin (10 μg/ml; Wisent Bioproducts, Saint-Jean Baptiste, QC), hydrocortisone (500 ng/ml; Sigma–Aldrich, St. Louis, MO), epidermal growth factor (EGF) (20 ng/ml; R&D, Minneapolis, MN), and cholera toxin (100 ng/ml; List Biological Laboratories, Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Then, adherent macrophages underwent further incubation in serum-free RPMI1640 at 37°C for 2h and subsequent treatment with LPS (List Biological Laboratories, Escherichia coli 055:B5), …

• Product #203A – LPS from Escherichia coli O55:B5

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of myelin-reactive Th17 cells ex vivo and the adoptive transfer EAE model

… To induce EAE by adoptive transfer, CD4+ T cells were isolated from the MOG35-55-specific Th17 cell culture with the CD4+ T Cell Isolation Kit 5 days after stimulation. CD4+YFP+ T cells further purified on a FACSAriaTM III were adoptively transferred into Tcrb−/− mice via tail vein injection (5 × 106 cells/injection). One day after transfer, recipient mice were immunized with MOG35-55 peptide in CFA and then given an intraperitoneal injection of pertussis toxin (PTX, 500 ng/injection) (List Biological Laboratories, Campbell, CA, USA)12. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

Male MeCP2 WT and MUT mice, 9 weeks old, were anesthetized via i.p. with a mixture of xylazine and ketamine (16 mg/kg and 80 mg/kg respectively) and immunized subcutaneously at the right and left flanks with 200 μl of an emulsion containing 200 μg of myelin oligodendrocyte glycoprotein peptide (MOG35–55, NH2-MEVGWYRSPFSRVVHLYRNGK-COOH; synthesized at the Johns Hopkins University Synthesis & Sequencing Core Facility, Baltimore, MD, USA). The peptide was dissolved in sterile water at 2 mg/ml, mixed at a 1:1 ratio with complete Freund’s adjuvant (CFA, Sigma-Aldrich Co., St. Louis, MO, USA), supplemented with 4 mg/ml of Mycobacterium tuberculosis. Pertussis toxin (200 ng List Labs, USA) was dissolved in 100 μl of phosphate-buffered saline (PBS) and injected i.p. the same day of the immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)