Bacterial Toxin Research Citations

2.2 Drugs

… Diphtheria toxin (List Biological Laboratories) was dissolved in saline and administered at 50 mg/kg, based on previous reports …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.3. LT Neutralization Activity (LTNA)

Recombinant PA and LF were purchased from List Biological (List Biological Laboratories Inc., Campbell, CA, USA). The ability of participant plasma to neutralize LT (LTNA) was adapted from the standard procedure from the CDC as previously described [29] using the J774.1 macrophage cell line (ATCC, Manassas, VA, USA). In brief, cells were plated overnight at 90,000 cells/well in a 96-well plate. Plasma samples were diluted 1:50 in culture medium, and then diluted for eight additional serial two-fold dilutions in dilution tubes, and then incubated with LT for 30 min at 37 °C (600 ng/mL of PA, 120 ng/mL of LF) before adding to cell cultures. Control wells included cells alone, cells with added PA only, LF only, or cells with PA and LF (LT). …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

EAE Induction

Mice were injected s.c. at the tail base with an emulsion containing equal amounts of complete Freund’s adjuvant (CFA) (Difco) containing 5 mg/ml Mycobacterium tuberculosis (strain H37Ra) (Difco) and 200 µg MOG35-55 in PBS. 400 ng of pertussis toxin (List Biological Labs) was injected i.p. on days 0 and 2 following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.1. Materials and Reagents

… Diphtheria toxoid (DT), pertussis toxin (PT), and TT used as coating antigens for ELISA were purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae
• Product #190 – Tetanus Toxin from Clostridium tetani

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice

C57BL/6 mice were either home-bred or were purchased from SamTako Ltd (South Korea). B6.SJL, Pmel Tg , Il17a Cre mice were purchased from Jackson Laboratory. Foxp3 DTR mice were kindly provided by Dr. Sin-Hyeog Im (POSTECH) with the permission of Dr. Alexander Rudensky (Memorial Sloan Kettering Cancer Center).23 R26 DTA mice and R26 YFP mice were previously described.24 25 Il17a Cre ×R26 YFP , Il17a Cre ×R26 DTA, and Il17a Cre ×R26 DTA/YFP mouse systems were created by breeding the relevant strains. CCSP Cre ×K-ras G12D mice have been described.7 Male or female mice of 8–12 weeks were used, and each experiment was conducted using age-matched/sex-matched groups. For DTR-mediated depletion experiments, 500 µg of diphtheria toxin (List Biological Laboratories) was intraperitoneally treated every other day after tumor injection in Foxp3 DTR mouse systems. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Induction and assessment of EAE

EAE was induced by subcutaneous injection with 200 μg of mouse MOG35–55 peptide (Prespec) emulsified in complete Freund’s adjuvant containing heat-killed Mycobacterium tuberculosis H37RA (BD Difco) on day 0. Pertussis toxin (200 ng; List Biological Laboratories) was intravenously injected on the day of immunization and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★Methods
Key resources table

Lipopolysaccharide from Salmonella Minnesota List Labs Cat#R595

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Pertussis toxin (150 ng per mouse, list labs) was given i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

MATERIALS AND METHODS.

4.1 Reagent.

… Purified LPS was purchased from List Biological Lab. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Cell cultures

… Established cancer cell lines were cultured according to ATCC recommendations. DT22 cells and BM-MSCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10%fetal bovine serum (FBS). MCF10A cells were cultured in DMEM/Ham’s F12 medium supplemented with 5% horse serum (Gibco BRL, Waltham, MA), insulin (10 μg/ml; Wisent Bioproducts, Saint-Jean Baptiste, QC), hydrocortisone (500 ng/ml; Sigma–Aldrich, St. Louis, MO), epidermal growth factor (EGF) (20 ng/ml; R&D, Minneapolis, MN), and cholera toxin (100 ng/ml; List Biological Laboratories, Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae