Citations

… Clostridium difficile toxin B (TcdB) (List Biologicals ): Spin down toxin tube for 30-45 s at 20,000 g. Reconstitute 20 g of TcdB into 100 L of sterile double-distilled water to obtain a 200 g/mL stock solution. Store at 2-8 C (see Note 5 ). …

• Product #155 – Toxin B from Clostridium difficile

… The sections were blocked in 10% NHS in PBS (30min) then incubated overnight in either mouse anti-TH, (1:20000, ImmunoStar, Hudson, WI, USA, antibody registry: AB_572268) or goat anti-CTB (1:20000, List Biological Laboratories Inc., antibody registry: AB_10013220) in 2% NHS in PBS at RT. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

–Toxin from Clostridium septicum List Biological Laboratories, USA

EAE: 

… injected with PBS (control) or 100 µg of MOG 35-55 (New England Peptide) emulsified in 100 µl complete Freund’s adjuvant containing 300 g Mycobacterium tuberculosis (Fisher Scientific), and with 200 ng pertussis toxin (List Biological Laboratories), intraperitoneally …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. …

Author did not specify which List Labs E. coli LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Induction of EAE and Therapeutic Study:

EAE was induced as previously described … Additionally, 200 ng of pertussis toxin was administered as an intraperitoneal injection on the same day of immunization (day 0) as well as day 2 post-induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Chicken polyclonal IgY (753A) against TcdA was purchased from List Biological Labs. …

Indirect IFA:

… To test the membrane location of TgATPaseP-GC, the IMC was separated from the plasma membrane by treating extracellular parasites with -toxin from Clostridium septicum (20 nM, 2 h) (List Biological Laboratories), followed by fixation on BSA-coated coverslips and antibody staining. In both cases, the standard immunostaining procedure was performed subsequently.

… The vaccine contained a mixture of … and 1 µg cholera toxin as an adjuvant (List Biological Laboratories, Campbell, California, USA) suspended in phosphate-buffered saline. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE:

Mice from each group were immunized subcutaneously after a minimum of 30 days post TAM administration89 with 100 µg of MOG p35-55 peptide (2 mg/mL in PBS) emulsified in Complete Freunds Adjuvant containing 2 mg of heat-killed Mycobacterium tuberculosis (H37RA, Difco Laboratories). MOG p35-55 was synthesized by Genemed Synthetics (Genemed Synthetics Inc., San Antonio, TX, USA). Mice also received intraperitoneal injections with 100 ng – 250 ng of Pertussis toxin (List Biological Laboratories) on the day of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)