Bacterial Toxin Research Citations

Denervation

… Four week old mice were given two intraperitoneal injections of 1 µg of Diptheria toxin (DT; List Biological Laboratories, Campbell, CA) in 100µL PBS at three to four day intervals. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Viruses and Tracers

SPNs were targeted by infusing the retrograde tracers Cholera-Toxin B (CTB; 1% in dH2O; List Biological Laboratories) and Fluorogold (FG; 3% in saline; Fluorochrome) into the GPe and SNr, respectively. Both tracers were infused at a total volume of 82.2nl and a rate of 23nl/minute. Volumes were selected based on pilot studies designed to contain the spread of tracers within each region. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… PLP/CFA emulsion was administered to four different regions above the shoulders and the flanks on Day 0 followed by intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) on Days …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vitro characterization of innate immunity and T cell immunity of viral vectors

… As a positive control, cells were exposed to 10 ng/ml Salmonella minnesota ultra-pure lipopolysaccharide (LPS, List Biologicals Laboratories, Campbell, CA). …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… After baseline measurements with normal lungs, mild endotoxemia was induced by continuous infusion (2.5 ng/kg/min) of lipopolysaccharide (Escherichia coli O55:B5, List Biological Laboratories Inc, Campbell, CA) …

• Product #203A – LPS from Escherichia coli O55:B5

The EAE model was performed as previously described. In brief, each mouse was injected subcutaneously (s.c.) with 115 g MOG35-55 peptide (Washington Biotech) emulsified in CFA (263810, Difco) plus an intraperitoneal (i.p.) injection of 300 ng pertussis toxin (NC9675592, List Biological) on days 0 and 2. Clinical signs were assessed daily as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE

On day 0, C56BL/6 mice received a subcutaneous injection in both rear flanks containing 200 µg of MOG35-55 peptide (MOG) (Amino acid sequence: M−E−V−G−W−Y−R−S−P−F−S−R−VV−H−L−Y−R−N−G−K; Synpeptide, Shanghai, CHN) and Complete Freund’s adjuvant (CFA) (Difco, Detroit, MI, USA), which contains 500 µg of desiccated non-viable M. Turberculosis (Difco, Detroit, MI, USA). Simultaneously, they received an intraperitoneal injection of 200 ng of Pertussis Toxin (PT) (List Biologic, Campbell, CA, USA). Mice received a second dose of PT two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunofluorescence staining

For immunofluorescence staining, 30-µm-thick frozen sections were incubated with the following primary antibodies: rabbit anti-serotonin (5-HT, 1:2000, Sigma #S5545), rabbit anti-TH (1:500, Millipore #AB152), rabbit anti-tropomyosin receptor kinase C (TrkC, 1:500, Abcam ##ab43078), and mouse anti-synaptophysin (Syn, 1:1000, Millipore #MAB5258), rabbit anti-glia fibrillary acidic protein (GFAP, 1:1000, Abcam, #ab7260) and goat anti-cholera toxin B (CTB, 1:2000, List Biological Laboratories, #703). Signal was detected with the corresponding second antibodies conjugated to Alexa Fluor 405 or 488 or 594 fluorescence (1:1000, Invitrogen).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… At 0 and 24 h after immunization, mice were intraperitoneally injected with pertussis toxin (List Biological, Campbell, CA). For adoptive transfer, unless stated otherwise, recipient mice were injected via the caudal vein with 5 106 MOG3555- specific CD8+ or CD4+ enriched T cells prepared as described previously in 0.2 mL phosphate buffer saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction. Active EAE was induced by subcutaneous (s.c.) injection of 100 μg MOG35-55 peptide (United Biochemical Research) in 50 μL of CFA containing 5 mg/mL Mycobacterium tuberculosis H37Ra (Difco Laboratories). Mice also received intraperitoneal injections of 200 ng Bordetella pertussis toxin (List Biological) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)