Bacterial Toxin Research Citations

Reagents:

… Ultrapure LPS was purified from Salmonella minnesota R595 (List Biological Laboratories) …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… the half corneal button securely attached to the bottom of the plate, and 1.5 mL of DMEM (Life technology) with 10% serum containing 40 g/mL gentamicin (Life technologies, NY), 1% ITS (Fisher Scientific, PA), and 100 ng/mL cholera toxin (LIST Biological Laboratories) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Infection and stimulation of monocytes:

… Cells were stimulated with 100 ng/ml ultrapure E. coli LPS (List Biological Laboratories, Campbell, CA) and 5 mM ATP (Sigma-Aldrich, St. Louis, MO) for the last 30 min of culture, as indicated. Mock treatment was the addition of the equivalent volume of media (without parasites or LPS) to cells. At the indicated time point, monocytes were pelleted by centrifugation at 500 x g for 5 min. Collected cells were stained, fixed, or lysed accordingly, as described below.

Author did not specify which ULTRA PURE E. coli LPS was utilized; List Labs provides the following:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4
• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

… Cholera toxin (CT) List Biological Laboratories Cat#100B …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Ultrapure LPS (Escherichia coli 111B4) List Biological Laboratories. INC Cat# 421…

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

THP-1 differentiation and stimulation.:

… The differentiated THP-1 cells were stimulated with the addition of 1.510⁷ CFU/well of live or heat-inactivated bacteria added in isolation or in combination with 1µg/ml purified CT or CTB (both from List Biologicals, Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… CT (10 µg/ml, List Biological Laboratories) was dissolved alone or in combination with CpG-ODN and curdlan in PBS, emulsified with IFA, and then injected intramuscularly as described above. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experimental Autoimmune Myocarditis:

… For this study, mice were backcrossed onto the BALB/c background as it is permissive for myocarditis [51]. In addition, the mice were injected intraperitoneally with 500 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS. The mice were monitored for at least one week following the injections. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Laboratories Cat# 181…

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)