Citations

Immunohistochemistry:

… goat anti-CTB (Cat# 703, List Biological Lab, 1:4000) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… and CTB, some sections were incubated for 3 days with a mixture of rabbit anti-DsRed (Clontech) and goat anti-CTB antibodies (1:10000 dilution; List Biological Laboratories Page 11. 11 The anti-CTB antibody is a commercial polyclonal antibody (List Biological …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… For the induction of EAE, incomplete Freunds adjuvant (IFA) and killed Mycobacterium tuberculosis strain H37RA were purchased from Difco (Sparks, MD), and pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… animal received bilateral injections of two different tracers in HD and HI, namely biotinylated dextran amine (BDA, 10,000 molecular weight, Molecular Probes, Eugene, USA) on the right and cholera toxin B subunit (CTB, choleragenoid, List Biological Laboratories …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Protocols in mice:

Six week-old female BALB/c mice (Charles River Laboratories, Saint Germain sur Arbresle, Rhne, France) were distributed in 5 groups (5 per group). Three groups were sensitized to EW by the intragastric administration of 5mg of EW on a protein basis and 10g of cholera toxin (CT, List Biologicals, Campbell, CA, USA), during 3 successive days on the first week and once per week for the subsequent 5weeks (Li et al., 2000). Sham-sensitized mice received CT and nave mice just received PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Endotoxin:

All endotoxin lots were derived from an E. coli Type O113:H10:K-negative strain of bacteria. Endotoxin was stored at the investigational site in a dedicated fridge at 28C, and reconstituted prior to administration, both according to the manufacturers instructions. The temperature of the fridge was constantly monitored and logged using an automated system. Lot #1188844 was derived from bulk material produced by the National Institute of Allergy and Infectious Diseases and FDA in 1967 and vialled in 2006 under GMP conditions by the Pharmaceutical Development Section of the NIH (Bethesda, MD, USA). The lots #94332B1 and #94332B4 were both derived from the same new GMP-grade bulk material and vialled under GMP conditions by List Biological Laboratories, Inc (Campbell, California, USA). This GMP-grade endotoxin has previously shown to be safe for administration in humans.

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

MOG Peptide-EAE Induction:

After 4 weeks on their experimental diets, EAE was inducedin mice (12-week-old) by trained MBP technicians. EAE wasinduced by subcutaneousflank administration of emulsifiedmyelin oligodendrocyte glycoprotein peptide 3555 (MOGpeptide; New England Peptide, Gardner, MA, USA) in com-plete Freunds adjuvant containing 5 mg/mL killedMycobac-terium tuberculosis(Becton Dickinson Difco, Franklin Lakes,NJ, USA) on Day 0 followed by the intraperitoneal administra-tion of 200 ng pertussis toxin (List Biological Laboratories,Inc., Campbell, CA, USA) on Days 0 and 2 to produce fea-tures seen in MS, such as axonal vacuolization and fragmenta-tion and spinal cord atrophy (Soulika et al., 2009). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Furthermore, mice received intraperitoneal injection of 300 ng pertussis toxin from Bordatella pertussis (List Biological Labs, Campbell, CA, USA) on the day of and 48 hours after the EAE induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… We performed an intraocular injection (left eye) of 80 µl of an aqueous solution of CTb (List Biological Laboratories, Inc., Campbell, CA) 1mg/ml in 0.1M phosphate buffer, pH 7.4 containing 10% dimethylsulfoxide to increase the permeability and uptake of this substance by retinal ganglion cells …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cholera toxin (CTB; List Biologicals) and FluorGrome (FG; Fluorochrome) retrograde markers were administered to MBH (CTB) and LH (FG) to express mPFC hypothalamic cells. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt