Bacterial Toxin Research Citations

Retrograde tracer injections:

Injections of cholera toxin beta sub-unit (CTb; List Biological Laboratories, Campbell, CA) were used to retrogradely label premotor cells with axonal projections to the XII motor nucleus, …

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 l of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na₂EDTA, 0.003 M NaN_3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Mass spectrometry:

Four-week old mice were euthanized, globes were exenterated and sterilized in 2% iodine (Sigma-Aldrich, St Louis, MI), after which the limbus was dissected, placed epithelium-side-down in 6-well culture plates (Corning Inc., Corning, NY), and incubated in defined keratinocyte serum-free media (dKSFM; Gibco, Fremont, CA) containing 50nM recombinant human epidermal growth factor (Peprotech, Rocky Hill, NJ), 500nM Cholera Toxin (List Biological Labs Inc., Campbell, CA) and 100 U/ml Penicillin-Streptomycin (Thermo Fisher Scientific, Waltham, MA) at 37C in 5% CO_{2. …}

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… CTb Gt Polyclonal List Biological Labs 102946-502 7032A10 1:5,0001:10,000 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (300 ng/200 µL; 180, List Biological Laboratories) was injected intraperitoneally (ip) on days 0 and 2 after MOG immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:

… For active immunization mice were immunized subcutaneously into the flanks with 200 µg MOG35-55 peptide (Anawa) emulsified in complete Freunds adjuvant (CFA; InvivoGen) and 200 ng pertussis toxin (List Biological Laboratories Inc.) intraperitoneally on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (200 ng; List Biological Laboratories, Campbell, CA, USA) was intraperitoneally administrated at 0 and 48hr postimmunization. EAE scores were assessed daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For adoptive transfer of effector T cells, 5×106 6 Th17 or Th1 1C6 T cells 7 were injected i.p., in cold PBS (Cellgro) into NOD.Scid recipient mice, which additionally received 200 ng of pertussis toxin (List Biological Labs) i.p. on d0 and d2 post-injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cells were incubated for 30 minutes at 37°C under normal cell culturing conditions, after which 10 ng/mL of pertussis AB5 holotoxin(List Biological Laboratories Inc., 179A) was added to the cells and incubation was continued for 2 h. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Primary human cell cultures:

Human skin samples were obtained as anonymised surgical waste, typically from abdominoplasty or mammoplasty. Briefly, skin biopsies were minced and trypsinized (0.05% trypsin/0.01%EDTA) at 37C for 4 hr. Cells were collected every 30 min, plated (2.5  10^4/cm²) on lethally irradiated 3T3-J2 cells (2.4 X 10⁴/cm²) and cultured in 5% CO2 and humidified atmosphere in keratinocyte growth medium (KGM): DMEM and and Hams F12 media (3:1 mixture) containing Fetal Bovine Serum (FBS) (10%), insulin (5 g/ml), adenine (0.18 mM), hydrocortisone (0.4 g/ml), cholera toxin (CT, 0.1 nM), …

STAR★Methods
Key Resources Table
Chemicals, Peptides, and Recombinant Proteins
QD Cholera Toxin List Biological Cat# 9100B

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Macrophages were activated with Salmonella minnesota R595 LPS (100 ng/ml; List Biological Laboratories) 4 hours before nigericin treatment or 16 hours before bacterial infection, unless otherwise noted. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)