Citations

… Activation of the NLRP1 inflammasome was achieved by treatment for 16 h with anthrax lethal toxin (2.5 lg/ml Anthrax Protective Antigen and 1 lg/ml Anthrax Lethal Factor mix, List Biological Laboratories, 171D and 169A). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Quantification of anti-CtxB and anti-OSP responses:

Anti-CtxB and anti-OSP responses were measured by previously described isotype-specific ELISAs [32,33]. Briefly, 96-well plates (Nunc) were coated with 1 g/mL solution of bovine GM1 monosialoganglioside (Sigma) in 50mM carbonate buffer overnight. Next, 1g/mL CtxB in 0.1% BSA/PBS purified from the classical Inaba strain 569B (List Biological Laboratories) was layered onto the GM1-coated wells. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Mice also received 250 ng pertussis toxin (List Biological Labs) via tail vein injection at the time of MOG3555/CFA immunization and again 2 d later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

… On Days 0 and 2 after immunization, 200 ng of pertussis toxin (List Biological Laboratories) were injected intraperitoneally. EAE clinical symptoms were scored (0, no clinical signs; 1, weak tail; 2, flaccid tail; 3, partially weak hind limb; 4, total hind limb paralysis; and 5, hind and fore leg paralysis).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To induce HC death in Atoh1-DTA mice, tamoxifen (Sigma-Aldrich) was dissolved in 100% corn oil and injected IP at 3 mg/40 g on P0 and P1. For fate-mapping experiments, tamoxifen (3 mg/40 g) was injected IP at P0 only to induce tdTomato expression in SC subgroups, followed by injection of DT (6.25 ng/g, IM; List Biological Laboratories, Inc.) at P1 to induce HC death in Pou4f3DTR mice …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental autoimmune encephalomyelitis:

Naive (CD4⁺V11⁺CD25^–CD62L^highCD44^low) CD45.1 T cells (5.010⁴) from MOGTCR-transgenic (2D2) mice were adoptively transferred with or without 5.010⁵ in vitro-cultured OT-II memory-like T_H17 cells into Rag2^-/- mice. After transfer, the mice were immunized with 100µg of MOG₃₅₅₅ peptide in complete Freunds adjuvant (Difco, Detroit, MI, USA). At 0and 48h after immunization, the mice were intraperitoneally treated with 200ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

DT administration:

DT (List Biologicals Laboratories) was suspended in PBS. Stock solutions were thawed once, and mice were injected i.p. with DT as previously described …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Active EAE Induction

MOG35–55 peptide with amino acid sequence MEVGWYRSPFSRVVHLYRNGK was purchased from Mimotopes (Mimotopes, Victoria, Australia) and resuspended in 0.09% saline solution at a concentration of 2 mg/ml. Fresh complete Freund’s adjuvant (CFA) was reconstituted with 4 mg/ml non-viable, desiccated Mycobacterium tuberculosis H37 Ra (Cat#231141, BD, MD, USA) in incomplete Freund’s adjuvant (Cat#F5506, Sigma, Millipore-Sigma, MO, USA). Pertussis toxin from Bordetella pertussis in glycerol (Cat#179B, List Biological Laboratories, CA, USA) was freshly diluted in PBS to 3 μg/ml. MOG35–55/CFA emulsion was freshly reconstituted by mixing equal volume of 2 mg/ml MOG35–55 with CFA and vortexed for 30 min at room temperature. For each mouse, a total of 200 μl MOG35–55/CFA emulsion was subcutaneously injected at flank and scruff sites; 100 μl of 3 μg/ml pertussis toxin was administered intraperitoneally 1 h and 24 h following MOG35–55/CFA emulsion. Clinical scoring was performed starting 7 days following active EAE induction, every day until the completion of the experiment. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Vaccine preparation and immunization:

DPX-rPA and alum-rPA were provided by IMV Inc. (Halifax, Canada), but AVA was provided by the CDC. Test and vehicle/control article formulations were prepared fresh on the day of dosing in accordance with the method provided. DPX-rPA was prepared as previously described¹⁹ containing a poly I:C (Thermo Fisher) or Pam3CSK4 (EMC Microcollections) adjuvant and a 10:1 mixture of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine):cholesterol (Lipoid GmBH, Germany) or S100:cholesterol (Lipoid GmBH), see Supplementary Table 1 for complete formulation details for each figure. The source of rPA was DRDC, List Biologicals, or Pfenex Inc.²⁶ …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Two hours and forty-eight hours after immunization, all mice received an intraperitoneal injection of … pertussis toxin (List Biological Lab …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)