Citations

EAE induction:

For EAE induction, Th/+ animals were immunized s.c. with 50g MOG₃₅₅₅ per animal emulsified in CFA substituted with 5mg/ml H37Ra (DIFCO, Detroit, MI, USA). Four hundred nanograms of pertussis toxin (PTX) per animal (List Biological Laboratories, Campbell, CA, USA) were injected i.p. twice on day 0 and day 2 relative to immunization. EAE was induced in Rag1^/ mice by the adoptive transfer of 10 Mio MOG-specific 2D2 T cell blasts i.p., and animals were immunized s.c. with 10g MOG₃₅₅₅ in CFA the following day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Combined anterograde tracing and retrograde tracing:

Ipsilateral injections of BDA (Thermo Fisher Scientific, Waltham, MA, USA) into the LPB and CTb (List Biological Labs, Campbell, CA, USA) into the VTA (4 out of 10 rats received a successful injection), DR (4 out of 10 rats received a successful injection), PPT (4 out of 14 rats received a successful injection), LDT (4 out of 14 rats received a successful injection) or LC (4 out of 12 rats received a successful injection) were made stereotaxically by iontophoresis. In each rat, a single injection of CTb into these regions was made using a glass micropipette filled with 0.5% solution of CTb dissolved in 0.05M phosphate buffer (PB; pH 6.0). The driving current (56mA, 200ms, 2Hz) was delivered for 30-40min. After CTb injection, a single injection of BDA was made into the LPB via a glass micropipette filled with a 10% solution of BDA dissolved in 0.01M PB (pH 7.3). The driving current (56mA, 200ms, 2Hz) was delivered for 4060min.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Clostridium difficile toxin A was from List Biological Laboratories, Inc. (Campbell, CA). …

• Product #152C – Toxin A from Clostridium difficile

Immunization and challenge.

Mice were lightly anesthetized by the use of isoflurane for intranasal administration. For immunization with D27-pLpxL, mice were primed with intranasal inoculation of 2 × 106 CFU D27-pLpxL in 30 μl saline solution, boosted with the same dose of D27-pLpxL 30 days later, and rested for 60 days before challenge. For peptide immunizations, mice were inoculated intranasally with a 15-μl solution containing 1 μg cholera toxin (CT; List Biological Laboratory, Campbell, CA) as the adjuvant
and …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Each mouse also received 500 ng in 200µl of pertussis toxin (PTX) (List Biological Laboratories) intraperitoneally on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Following immunization 250 ng pertussis toxin (List Biological Laboratories, California) was given intraperitonealy (IP) and again 2 days later. Seven days following immunization mice received a second MOG immunization as described above in two alternate …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 1 µl of 0.2% Cholera toxin subunit b (CTb; List Biological Labs, Campbell, CA, USA) was pressure injected into the right VPMpc (1.9 mm anterior, 0.6 mm lateral, 4.2 mm ventral relative to bregma). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Proteins included … and recombinant protective antigen (rPA; List Biologicals, Campbell, CA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE:

… Mice were also intraperitoneally injected with 20 µg/kg of PTX (List Biological Laboratories) immediately and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 2% CTb Cabra [1:5000] (List Biological Labs) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt