Citations

… Pertussis toxin was purchased from List Biological Labs (Campbell, CA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Native PTx (in glycerol or lyophilized), … or genetically modified PTx (PTg) was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #184 – Pertussis Toxin Mutant

Immunizations and challenge in cotton rats:

Four to eight week old female cotton rats (Sigmodon hispidus) were immunized with vaccines consisting of 5µg RSV F protein previously described formulated in 5µg Cholera toxin [List Biological Laboratories, Campbell, CA; Cat. 100B], given two weeks apart. The vaccines were administered via a pipette directly to the mid-line of the right and left nostril in a volume of 10µl in sedated animals with ketamine/xylazine or isoflurane/oxygen anesthesia. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and clinical scores:

… Pertussis Toxin (PT; List Biological Laboratories Inc., Campbell, CA, USA) was intraperitoneally administered at a dose of 200 ng/mouse on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Unless stated otherwise, all reagents were obtained commercially from the following sources and used without further purification: … LPS (Escherichia coli-derived, serotype O111:B4; List Biological Laboratories …

• Product #201 – LPS from Escherichia coli O111:B4
   

… Triton X-100 (2h, room temperature) and then incubated in primary antibodies against CTB 276 (1:1000; List Laboratories catalog #703) and …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Effect of MSCs on LPS-driven monocyte activation:

… To assess the immunomodulatory ability of MSCs cultured with either 10% FBS or 10% ePL culture media, a cytokine production assay was performed according to established protocols [35]. Equine MSCs (n = 3) at P6 were placed at the bottom of 12-well transwell plates at 100,000 cells/well and allowed to adhere for 12 h in the presence of their corresponding medium. The following day, medium was aspirated and replaced with 1.5 ml of RPMI-1640 supplemented with 10% DHS. Half a milliliter containing 400,000 equine monocytes (ratio 1:4) from the three individuals (n = 3) were added to each insert of a transwell plate with pore size of inserts 0.4 m (Corning, NY), stimulated with 50 ng/ml of E. coli 0111:B4 LPS (List Biologicals Inc.) and incubated at 37 C under 5% CO₂. At 6 and 18 h time points, cell culture supernatants were collected and assayed for the production of the proinflammatory cytokine tumor necrosis factor (TNF)- as an indicator of inflammatory responses [27].

• Product #201 – LPS from Escherichia coli O111:B4

… Three groups were sensitized by oral gavage by the administration of 1 mg per mouse of either OVA, OM, or LYS, plus 10 µg of CT (List Biologicals, Campbell, CA, USA), and another three groups were polysensitized with a mixture of 1 mg each of the three proteins …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Vivo Incubation of CT in a Ligated Ileal Loop

… The segment was then injected with 300 μl of either sterile physiological saline (composition in mM: NaCl 118, NaHCO3 25, D-glucose 11, KCl 4.8, CaCl2 2.5, MgSO4 1.2, NaH2PO4 1.0) as a control, or CT (12.5 ug/ml; List Biologicals, Campbell, CA, USA) in physiological saline. This concentration of CT is within the range that effectively induced changes in gastrointestinal motility in male mice (Balasuriya et al., 2016), increased the excitability of specific enteric neuronal subtypes in guinea pigs (Gwynne et al., 2009; Koussoulas et al., 2017), and increased secretion in rat and human intestine (Burleigh and Borman, 1997; Kordasti et al., 2006). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

… – Pertussis toxin (PT; 500 ng; List Biological Laboratories, Inc., Campbell, CA, USA) was intraperitoneally injected at days 0 and 2 post-immunization (PI) PT (500 ng; List Biological Laboratories, Inc.) was injected intraperitoneally at days 0 and 2 PI …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)