Bacterial Toxin Research Citations

Goat anti-CTB List Biological Laboratories Cat# 703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… For FAP+ cell deletion, Diphtheria Toxin (DTx) (List Biological Laboratories) was administered by 390 i.p. injection to 8 week old FAP-DTR mice at a dose of 25ng/g, twice a day, in a prophylactic regime (at days -7 and -5 post STIA) and in a therapeutic regime (at days 3 and 5 or at days 5 and 7 post STIA or at days 10 and 12 393 post STIA). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

REAGENTS AND SOLUTIONS:

Anthrax protective antigen and anthrax lethal factor, 1 mg/ml

Dissolve anthrax lethal factor and anthrax protective antigen (List Biological Labo-

ratories, cat. no. 172B and 171E) each to a nal concentration of 1 mg/ml in sterile

dH2O. Divide into 10-l aliquots or an appropriate volume for the users experiments

and store up to 2 years at 80C. Avoid freeze-thaw cycles. Remove one aliquot of

each from the freezer 30 min prior to starting the experiment. Allow them to come to

room temperature. Enzymatically inactive anthrax lethal factor (List Biological Lab-

oratories, cat. no. 176) can be used as a negative control, if desired. Good biosafety

level 2 laboratory practices should be employed in the safe handling of this reagent.

Protective antigen and lethal factor should be stored separately.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #176 – Anthrax Lethal Factor (LF), Recombinant, Mutant E687C from B. anthracis

LPS, 1 mg/ml

Prepare a 1 mg/ml stock of Salmonella Minnesota R595 (Re) LPS (e.g., List Bi-

ologicals, cat. no. 434) in H2O containing 0.5% triethylamine. Divide into 100-l

aliquots and store up to 1 year at 4C.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… Control mice received only CFA. On day 0, experimental and control mice were also immunized with 50 ng of pertussis toxin (List Biologicals, Campbell, CA). The mice were killed at 1, 2, or 3 weeks after …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin was purchased from List Biological Lab (Epsom, England). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Ultra-pure lipopolysaccharide (LPS) from Escherichia coli O111:B4 (Cat# 421, List Biological, Inc., Campbell, CA) served as a positive technical control for cytokine secretion 132 (RAW264.7 cells, 2 ng/mL; …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Pertussis toxin List Biological Laboratories Cat #181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction and Evaluation of EAE:

… EAE was induced in PWD mice using the following modifications to the protocol above, as previously described (50). Mice were injected subcutaneously with 0.1ml of emulsion containing 2.5mg of MSCH in PBS and 50% CFA on day 0 and day 7. On day 0 and day 2, mice also received an i.p. injection of 200ng pertussis toxin (List Laboratories, USA) as an ancillary adjuvant. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. Mice were examined daily and scored for disease severity using the standard scale: 0, no clinical signs; 1, limp tail; 2, paraparesis (weakness, incomplete paralysis of one or two hind limbs); …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (400 ng; List Biological Laboratories, Campbell, CA) was administered intraperitoneally on days 0 and 2. Mice were monitored daily, and the disease was scored on a scale of 0- 5 as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)