Citations

BoNT/A Heavy Chain Administration and Groups:

Recombinant BoNT-A HC was purchased from List Biological Laboratories Inc. (Campbell, CA, USA).

Intermittent administration of BoNT/A HC applied in the study was done via two routes: (1) local application of the BoNT/A HC directly onto injury site (4 g/L in 16 L saline); and (2) administration of BoNT/A HC via a lumbar intrathecal catheter [46]. After establishing the rat model, 2 g/L of BoNT/A HC were administered every week to the SCI in the BoNT/A HC treatment groups. Animals were divided into the following groups, with six rats in each group: (1) the control (or pseudo surgery) group for which the skin incisions were made, the laminae were cut, and the spinal cords were exposed but not injured; (2) the SCI-only group for which unilateral (left side) lumbar spinal cord injuries were made (to prevent the vehicle intervention on BoNT/A heavy chain, the same volume of sterile saline was applied in SCI-only animal with same method and period); and (3) the SCI with BoNT/A HC treatment, in which BoNT/A HC was administered every week, and the injury was made. This group was further divided into four groups (with six rats each) based on different periods post-injury, i.e., two days, one week, two weeks and four weeks. …

• Product #612A – Botulinum Neurotoxin Type A Heavy Chain, Recombinant, Binding Domain

Toxin A inhibition assays:

Vero cells (CCL-81; ATCC, Manassas, VA) were maintained in complete media (MEM+antibiotic/antimycotic+10% FBS) in T-75 flasks at 37C, 5% CO₂. For TcdA inhibition assays, sterile 96-well tissue culture plates (Thermo-Fisher, Ottawa, ON, Canada) were seeded with ~210⁴ cells/well in a total volume of 200L of complete media. Plates were incubated for 24h at 37C, 5% CO₂. Media was then carefully removed from each well and replaced with 200L of fresh media, 20L of antibody (serially diluted in sterile PBS, giving final in-well concentrations ranging from 1M to 1.95nM) and 10L of TcdA (List Biological Laboratories; final in-well concentration of 10ng/mL corresponding to 32.5pM) for all wells. …

• Product #152C – Toxin A from Clostridium difficile

Cell culture studies:

… For toxin neutralization, we mixed different concentrations of CT (List Biological Laboratories, Campbell, CA) and nanoparticles, incubated for 1 h, and added the mixture to the cell monolayers. After 2 h, supernatants were collected and assayed for cAMP by enzyme immunoassay (Cyclic AMP ELISA Kit, Cayman Chemical Co., Ann Arbor, MI). All cAMP measurements were done without additional acetylation. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

In vivo effect of CT in mice

Transgenic mice +/- or -/- for the GalNAcT-gene as described above were fed with 10 μg CT (Azide free, List Laboratories) in PBS with 3% NaHCO3. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunofluorescence analysis:

… Consequently, sections were incubated with the following primary antibodies at 4C for 24 h: polyclonal rat anti-Cav3.1, anti-Cav3.2, (1:200 dilution; Alomone Labs, Jerusalem, Israel), Goat anti-CTB (cat. no. 703; List Biological Laboratories, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… MAPKKide was obtained from List Biological Laboratories (Campbell, CA 

Author did not specify which List Labs MAPKKide was utilized; the following are available:

• Product #530 – MAPKKide Peptide Substrate (o-Abz/Dnp) for Anthrax Lethal Factor
• Product #531 – MAPKKide Peptide Substrate (DABCYL/FITC) for Anthrax Lethal Factor
• Product #532 – MAPKKide Plus (AMC) Specific Substrate for Anthrax Lethal Factor

EAE:

… Two-hundred nanograms of pertussis toxin (PT; List Laboratories, Campbell, CA, USA) were injected intraperitoneally on days 0 and 2. For passive transfer EAE, donor mice were immunized as describe above. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Rat cortical neurons at DIV18-21 were treated with a concentration range of … BoNT/B (List Laboratories Inc., US) in triplicate wells for 24 h at 37°C. …

Sagittal sections were processed for immunohistochemical staining with primary antibodies against human nuclei (HuNA, 1:200, Chemicon) to identify for transplanted hGRP, GFAP (1:1000, Chemicon) for astrocytes, GFP (1:1000, Millipore) for ReST, serotonin (5-HT, 1:20,000 Immuostar) for descending raphespinal tract, anti-dopamine beta hydroxylase (DH, 1:1000, Chemicon) for descending coeruleospinal tract, anti-cholera toxin B subunit (CTB, 1:2000, List Biological Laboratories, Inc) for ascending sensory axons.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction and clinical evaluation of EAE:

EAE was induced in 11-week-old female C57BL/6J mice as described⁵⁷. … and intraperitoneal injections of 200ng pertussis toxin (#180-A1; List Biological Laboratories Inc., Campbell, CA) in 0.2ml of PBS on day 0 and day 2. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer