Bacterial Toxin Research Citations

Immunization:

For mucosal immunization, mice were anesthetized with isoflurane and then intrarectally administered 40 µl of sterile PBS containing 10 µg cholera toxin (List Biological Laboratories, Inc.; Campbell, CA) and …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Measuring the LPS induced response in IC-21 cells:

A 12-plex mouse inflammatory cytokine multi-analyte ELISArray kit (Qiagen) paired with LPS O111:B4 (phenol extract, Sigma Aldrich) was used to determine how many hours of LPS exposure was required to produce a measurable cytokine response. Limit of detection for the ELISAarray 12-plex kit is stated by the manufacturer as 10 pg/mL for each given cytokine. For further in-depth examination we chose ULTRA PURE LPS O111:B4 (List Labs) to avoid contaminates often found in other LPS preparations. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

…Pertussis Toxin (PTX) from List Biological …

Results:

… For each ligand, inhibition of cAMP accumulation was blocked by pretreatment with PTX (100 ng/mL), indicating that these were Gi-mediated effects (octreotide not shown) (Fig. 1C and 1D). These results demonstrate that in pituitary tumor cells, both octreotide and SOM230 had full efficacy for cAMP inhibition and this effect was fully inhibited by PTX. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Treatment of mice, and flow cytometry:

Four-week-old male C57BL/6 mice were intraperitoneally (i.p.) injected with 25g of LPS (List Biological Laboratories, Inc., Campbell, CA) …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Toxin Activity Inhibition Assay:

… The cytotoxic titer was defined as the reciprocal of the highest dilution that produced rounding in 80% of Vero cells for each sample. Vero cells treated with purified C. difficile toxins (A and B) and antitoxin (List Biological Labs, 152C and 155C; TechLabs, T5000) were used as controls. A test cytotoxicity assay was run prior to assays to ensure that the 2-AI molecules did not affect the cytoskeleton of Vero cells at the tested concentrations.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Pertussis Toxin Preparation:

Lyophilized pertussis toxin (List Biological) was reconstituted with distilled water. The reconstitute was then further diluted to a concentration of 2500 ng/ml then loaded into 1ml syringes for IP injection (Stromnes and Goverman, 2006).

EAE Induction:

Mice were shaved dorsally from the base of the tail to the middle of the back rostrally. Subcutaneous (SC) injections of MOG35-55/CFA emulsion were performed on day 0 and day 7. Each mouse received 4 subcutaneous (SC) injections of 0.05 ml at separate locations on the rump, yielding a total of 0.2 ml per mouse. Injections sites were arranged rostro-caudally, two on either side of the dorsal midline. A single IP injection 0.1ml of pertussis toxin was administered at a concentration of 2500 ng/ml on day 0 and day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin assay:

Pertussis toxin (List Biological Laboratories, Inc., Campbell, California) was activated by incubation …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis Toxin from B. pertussis, Lyophilized in Buffer List Biological laboratories, Inc Cat 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE Induction:

Active induction of EAE was performed by subcutaneous injection of nave 68 week B10.PL IL-3^/, IL-3^+/, and IL-3^+/+ littermate mice over four sites in the flanks with 200 µg MBP Ac1-11 (CS Bio, Menlo Park, CA, USA) emulsed in CFA (Difco, Becton Dickinson Co., Franklin Lakes, NJ, USA). Pertussis toxin (200ng) (List Biological Laboratories, Campbell, CA, USA) was injected i.p. at the time of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Flow cytometry:

Cells were trypsinized, rinsed in cold PBS-BSA, and labeled with m825-MMAE, an isotype-matched human IgG, or FITC-conjugated protective antigen (List Biologicals Laboratories) in PBS-BSA at 4^o C. …

• Product #175 – Anthrax PA 63 – FITC Conjugate