Citations

Reagents:

Purified recombinant verotoxin-1 (VT-1) was purchased from List Biological Laboratories, Inc. …

Intracellular immunofluorescence:

BAK and BAX conformational changes were tested by flow cytometry using conformational antibodies. Cells, treated or not with VT-1 for 6h, …

• Product #161 – Shiga Toxin 1 from Escherichia coli

Stimulators and inhibitor:

Ultrapure lipopolysaccharide (LPS) (List Biological) was used at 500 ng ml1 for 30 min or 16 h, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Two intraperitoneal injections of 400 ng pertussis toxin (List Biological Laboratories, Denver, CO) were given 24 and 72 hr later. Clinical scores (0 = healthy, 1 = flaccid 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin was purchased from List Biologicals (catalog #181).

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Diphtheria toxin (30 g/g of mouse; List Biological Laboratories, INC) was 285 injected subcutaneously either at postnatal day (P) 1 or P5 and the brains were 286 collected at various ages (Fig. 1a and Fig. 5a) 

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… After day 13 or 14 in vitro, half of the neuronal coverslips were treated with tetanus toxin (1 nM or 100 pM, List Biological Labs) at 37 C for 24 h. Untreated neurons were used as controls. …

• Product #190A – Tetanus Toxin from Clostridium tetani

Antibody assay:

IgG and IgA antibody responses to three bacterial pathogens, LPS from E. coli O-111B4 (E. coli-LPS) (ultra-pure E. coli-LPS, List Biological Laboratories, Campbell, CA), …  Briefly, human sera from both patients with RA and NL controls were diluted with ChonBlock^TM blocking/sample dilution buffer as follows: for IgG and IgA antibodies against E. coli-LPS: 1/1,000 & 1/1,000 (for both IgG and IgA), Pg-LPS: 1/10,000 & 1/500, PG-PS: 1/20,000 & 1/10,000, and IgG antibodies against CCP: 1/500. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Discussion:

The proportionality model of intoxication suggests the cytosolic stability of the toxin A chain will directly impact the capacity for recovery from intoxication, which is consistent with our observations for Dtx: as shown in Figs 56, cells could only recover from a minimal initial inhibition of protein synthesis by this relatively stable toxin35. Other reports further suggest the extent of intoxication is linked to the efficiency of toxin clearance from the cytosol. For example, cellular resistance to ricin or EtxA results from enhanced degradation of the cytosolic toxin33,34,36. Conditions that impede the turnover of cytosolic toxin likewise generate cellular sensitivity to ricin or Stx15,32,33,36. These collective observations indicate the extent of intoxication is directly linked to how much toxin is in the cytosol, with the amount of cytosolic toxin representing a balance between toxin delivery to the cytosol and toxin removal from the cytosol.
 

Toxins:

… EtxA and Dtx were purchased from List Biologicals (Campbell, CA). …

• Product #160 – Exotoxin A from Pseudomonas aeruginosa
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

LPS Challenge:

… On the morning of the LPS challenge, we injected each experimental bird intra-abdominally with 1 mg mL1 lipopolysaccharide from E. coli (O55:B5; List Biological Laboratories Inc.) dissolved in PBS. Eight hours post-injection, we collected blood samples from each experimental bird by puncturing the wing vein with a 26-gauge needle. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

EAE model:

C57BL/6J mice were immunized subcutaneously with 100 µg MOG₃₅₅₅ (MEVGWYRSPFSRVVHLYRNGK, AnaSpec Inc), in incomplete Freud adjuvant (IFA, Sigma) containing 400 µg of heat-inactivated Mycobacterium tuberculosis H37 RA (Difco) and received two intravenous injections (i.v.) of 350 ng of pertussis toxin (List Biological Laboratories Inc), on day 0 and day 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)