Bacterial Toxin Research Citations

Antigen-specific IgE:

Low abundance of serum antigen-specific IgE meant a modified, multiplexed Luminex assay (Luminex Corporation, Austin, TX) was employed to measure tetanus and acellular pertussis antigen-specific IgE responses. Sera were treated with GullSORB (Meridian Bioscience, Inc., Cincinnati, OH) to remove potentially competing IgG and incubated with purified pertussis toxin mutant (PTM), pertactin, or tetanus toxoid (all from List Biological Laboratory, Campbell, CA) coupled to fluorescent-barcoded MagPlex microspheres (Luminex Corporation). …

• Product #184 – Pertussis Toxin Mutant
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)
• Product #191 – Tetanus Toxoid from Clostridium tetani

Oral Sensitization and GI Antigen Challenge:

Oral allergic sensitization and oral allergen challenge were performed as depicted in Figure 1A. For oral sensitization, mice were given 1 mg of OVA (grade V, Sigma-Aldrich, St. Louis, MO, USA) and 15 µg of CT (List Laboratories, Campbell, CA, USA) in …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE and CBD Treatment Regimen:

Experimental autoimmune encephalomyelitis was induced in groups of 10 female C57BL/6 mice (68 weeks old) as described previously … Following immunization, 200 ng of pertussis toxin (List Labs, Campbell, CA, USA) was injected i.p. into mice on day 0, followed by a 400 ng pertussis toxin intraperitoneally (i.p.) injection on day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell transfection and infection:

Mycoplasma free-ZF4 cells (ATCC CRL-2050), established from 1-day-old zebrafish embryos, were grown at 28C in Dulbeccos modified Eagles medium (DMEM)/F12 (Gibco) medium supplemented with 10% fetal bovine serum (FBS; Gibco) in an atmosphere containing 5% (v/v) CO₂. These cells were infected by replacing the culture medium with Opti-MEM (Gibco) and adding E. piscicida at a multiplicity of infection (MOI) of 50. For CTB (List Biological Laboratories) treatment, Pam3CSK4 (InvivoGen) primed cells were stimulated with 20mg/mL CTB plus 1mg/mL ultrapure LPS (E. coli O111:B4, InvivoGen)^22,41. For inhibitor assays, the cells were pretreated with Z-WEHD-FMK (ApexBio), Z-YVAD-FMK (ApexBio), Ac-LEVD-CHO (Sigma), Z-VAD-FMK (Beyotime Biotechnology), Ac-DEVD-CHO (Beyotime Biotechnology), or 10mM glycine (Sigma), respectively, for 30min before being exposed to E. piscicida at an MOI of 50.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cholera toxin subunit B (CTb) Antiserum Cholera toxin B subunit List Biological Laboratories; Goat; Polyclonal CAT No. 703; Lot No. 7032A7 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Materials and Methods:

… Cholera toxin (CT) was purchased from List Biological Laboratories. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… PTX (Pertussis toxin) List biological laboratories, Campbell, CA, USA …

Mouse immunization with MOG35-55 and EAE induction:

AhRDC , AhRTreg , AhRAstro , AhRCD4 and AhRfl/fl control mice were actively immunized with a subcutaneous (s.c.) injection of 50 µl of MOG35-55 emulsified in complete Freunds adjuvant (CFA, composition detailed in table 3) per groin and per axilla (200 µg MOG35-55/animal). The s.c. inoculation was accompanied by an intraperitoneal (i.p.) injection of 400 ng of pertussis toxin (PTX) on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PA 63 was prepared from PA 83 using furin [52] or purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) in the purified form …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… On Day 10 all animals were anesthetized with isoflurane, mounted in a stereotaxic instrument, and received a unilateral injection of the retrograde tracer cholera toxin B (CTB; List Biological Laboratories; 1% solution) into the BLA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Tracer injection protocol.:

As previously described [21], glass micropipettes … were filled with 1% desalted CTb (List Biological Lab.) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt