Citations

EAE:

… On days 0 and 2 post-immunization, 200 ng of pertussis toxin (PT) (List Biological, Campbell, CA) was administered intraperitoneally (ip) in 0.2 ml of PBS (Sigma, St. Louis,Minnesota). Mice were observed daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… CRM (List Biological Laboratories Inc., Campbell, CA) …

• Product #149A – Diphtheria Toxin CRM197, Mutant

EAE induction.

Adult mice were injected s.c. in each hind leg with 50 μg of MOG35–55 (Peptide Synthesis Facility, University of Alberta) emulsified in 100 μl CFA (MilliporeSigma). Animals received 0.3 μg/ml of pertussis toxin injected i.p. Injection of pertussis toxin (List Biological Laboratories) occurred on the day of induction with MOG35–55, with subsequent infection 48 hours later. Control animals were immunized with CFA and PBS (1:1) and pertussis toxin, as above. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and treatments:

… EAE was induced using a modification of our previously reported method [8, 9]. In brief, 8 to 10 weeks old C57BL/6 female mice were subcutaneously injected with 100 µg myelin oligodendrocyte glycoprotein (MOG) ₃₅₅₅ emulsified in complete Freund’s adjuvant (CFA) supplemented with intraperitoneal injections of 400 ng pertussis toxin (List Laboratories, Campbell, CA, USA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and clinical evaluation:

For the induction of EAE, wild-type (WT) and Slfn8^/ mice (C57BL/6 and 129SV mixed background) were immunized subcutaneously with 150 µg of myelin oligodendrocyte glycoprotein (MOG) peptide (MOG_35-55) (Anaspec, Fremont, CA, USA) emulsified in CFA (day 0). After immunization, the mice were intraperitoneally (i.p.) injected with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization of Mice:

… Five mice per group were anesthetized with ketamine and immunized three times at a 2-week interval by i.n. injection of 20µg of ovalbumin (OVA; Sigma, St. Louis, MO) alone, or mixed with 10µg of TCTA1T and TmCTA1T, or 2µg of CT (List Biological Laboratories Inc., Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Antibodies and reagents:

… Shiga toxin 1, Shiga toxin 2, cholera toxin, and pertussis toxin were purchased from the List Biological Laboratories. …

Online supplemental material:

Fig. S1 shows that Tg, but not Tu, BFA, or MG132, induces IRE1 to undergo phosphorylation at S729. Fig. S2 shows that KIRA6 rapidly suppresses SubAB-induced S729 phosphorylation of IRE1 and that Shiga toxins, cholera toxin, and pertussis toxin do not induce S729 phosphorylation even when used at high concentrations. …

• Product #161 – Shiga Toxin 1 from Escherichia coli 
• Product #162 – Shiga Toxin 2 from Escherichia coli

Author did not specify which Cholera toxin was utilized.  List Labs provides Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae and Product #101 – Cholera Toxin from Vibrio cholerae.

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Reagents and antibodies:

Cholera toxin (100) and insulin (16634) were from List Biological (Campbell, CA, USA) and Invitrogen (Carlsbad, CA, USA), respectively. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In addition, the mice received 100 ng of pertussis toxin (List Biological Laboratories) iv on days 0 and 2 and were treated ip with 100 mg of anti-TIGIT (clone 4D4, 1G9, or 1B4) or isotype control (Arme- nian hamster IgG or mouse IgG1) on days 0, 2, 4, 10, and 17 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Stimulation and ERK Phosphorylation

Jurkat cells stably expressing wild-type CCR6 (WT-CCR6) or mutant CCR6 were fasted at a density of 2 × 106 cells/ml for 4–6 h in fasting medium (RPMI medium containing 0.5% BSA, 2 mM l-glutamine, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, 5.5 µM β-mercaptoethanol, and 10 mM HEPES) with or without 0.1 µg/ml pertussis toxin (PTx) (List Biological Laboratories, Campbell, CA, USA) in a 37°C incubator. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)