Citations

Materials:

LPS from Salmonella typhimuirum (no. 225) was purchased from List Biological Laboratories (Campbell, CA). …

Cell culture and stimulation:

…  LPS (100 ng/ml) was added to cells during both the overnight pulse and chase period.  Lysosomal damage was induced by feeding BMM 3 m AW beads in RPMI lacking serum for 60 min.  AW beads were added at a
concentration empirically determined to result in uptake of on average 3 to 4 beads per cell by
both resting and LPS-activated BMM.  All analyses of damage were performed on cells that had
internalized 3 to 7 beads per cell. …

• Product #225 – LPS from Salmonella typhimurium

EAE:

Six- to eight-week-old, sex-matched WT and SRC1/ littermates were injected s.c in the rear flank with 100 µg MOG3555 peptide (2HN-MEVGWYRSPFSRVVHLYRNGK-COOH) in complete Freunds adjuvant (Sigma), and 250 ng pertussis toxin (List Biological) was injected i.p. Mice were monitored daily, and disease severity was scored. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

RGC density after retinal IRI:

We applied the anterograde tracer, FITC-conjugated cholera toxin B (CTB-FITC; List Biological, Campbell, CA) to count the number of surviving RGCs at one week after retinal IRI 

• Product #106 – Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate

EAE:

On day 0, donor mice were actively induced with EAE via s.c. immunization in the flank region with 200 µg MOG3555 (MEVGWYRSPFSRVVHLYRNGK; RP10245; GenScript) in CFA (263810; BD Difco). Pertussis toxin (200 ng) from Bordetella pertussis (179B; List Biological Laboratories) in PBS was administered i.p. After induction of EAE, mice were observed daily for weight loss, disability, and availability of food and water. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and Surgical Procedures:

… Three days before perfusion of the animals, 5 µl of 1% cholera toxin B subunit (CTB, List Biological Laboratories) was injected into the left sciatic nerve at hip level to trace the axons. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

2.2. Preparation of Antigen and Induction of EAAU

Melanin-associated antigen (MAA) was prepared according to the method proposed by Broekhuyse et al. [8, 11]. To induce EAAU, the rats were given two simultaneous injections of different MAA preparations. First, MAA was suspended in phosphate-buffered saline (PBS) and 1 : 1 emulsified in complete Freund’s adjuvant (Sigma Aldrich, St. Louis, MO, USA). The suspension (0.05 mL) was injected into the left hind footpad. Second, MAA was emulsified with 1 μg of purified Bordetella pertussis toxin (List Labs, Campbell, CA, USA) and injected into the peritoneal cavity for a total volume of 0.05 mL.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CTb Goat, antiserum 1:3000 choleragenoid Cat. #703, List Biological Laboritories (Thompson and Swanson 2010)

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Oral DMF Gavage and EAE Induction

… Induction of active EAE was performed as previously described (38, 39). For DC transfer experiments, 1 × 105 DC, cultured in vitro for 10 days with or without 200 µM MMF (Sigma-Aldrich) and stimulated with 1 µg/ml lipopolysaccharide (LPS) (Sigma-Aldrich) for 24 h, were injected intraperitoneally at d2 and d7 after EAE induction together with 200 ng pertussis toxin (List Biological Laboratories).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals and Kits:

… Lipopolysaccharide from Eschericha coli 055:B5 List Biological Labs, Incorporated …

Spin trapping coupled to EPR:
 
… This technique was used in this project to evaluate the ROS species release from cells after stimulation by LPS, and if this environment was modified by treatment with BSO. BMPO (5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide) was used as a spin trap. It specifically reacts with O2.-  forming a superoxide adduct, BMPO-OOH, or with HO. forming the hydroxyl adduct, BMPO-OH which is characterised by a 4 lines absorbance spectrum (Figure 2.2). …

Cell preparation:

RAW Cells were plated at 5x106cells in 5ml of RPMI media supplemented with 10%FCS and 1%P/S into 25cm2 flasks. At 70% confluence, 120M of BSO, an inhibitor of the synthesis of GSH, was added to the culture for overnight incubation. The next day, cells were stimulated for 2h with LPS. In total four experimental conditions were tested: control, LPS, BSO, BSO+LPS.  

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Briefly, heat-extracted CFA/I, 389 CS1, CS2, CS3, CS4 or CS5 fimbriae (200 ng per well) and LT (List Biological Laboratories, Inc., Campbell, CA; 100 ng per well) were coated to wells of 2HB plates …

Lethal Toxin (LT) consists of LF + PA:

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis