Bacterial Toxin Research Citations

…For CTB injections, a glass micropipette … filled with 1% low salt CTB (List Biological Laboratories, Campbell, CA) in distilled water was stereotaxically … 4°C, and then in TBS containing goat anti-CTB (1:100,000; List Biological Laboratories …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…in 6 week- old mice subjected to a sensitizing protocol consisting of the oral administration of the amount equivalent to 5 mg of protein per mouse and 10 µg of cholera toxin (CT, List Biologicals, Campbell, CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

LPS transfection:

LPS transfections were performed as previously described (Hagar 2011), with modifications. Briefly, 100 ng of rickettsial LPS or Salmonella LPS (List Biologicals) was added to Opti-Mem (Gibco), and 50 ng of Lipofectamine 2000 (Invitrogen) or DOTAP (Thermo-Scientific) was added to Opti-Mem and allowed to equilibrate for 5 min at room temperature. The LPS and Lipofectamine/DOTAP mixtures were combined and allowed to form complexes at room temperature for 30 min. Cell culture medium of BMM were replaced with Opti-Mem, and the LPS-transfection reagent complexes were added. Cells were centrifuged at 300 x g for 5 min, and then incubated at 4 hours at 37C. Cell medium was replaced with DMEM/F12 and 10% v/v fetal bovine serum for 20 hours. Cell lysates and medium were collected after a total of 24 hours post-transfection.

Author did not specify which List Labs Salmonella LPS was utilized.  List Labs provides the the following Salmonella LPS:

• Product #225 – LPS from Salmonella typhimurium
• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)

EAE:

…On the day of immunization and 48 h later, 200 ng pertussis toxin (List Biological Laboratories, 181) in 250 µl sterile phosphate-buffered saline (PBS) was injected intra- peritoneally. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

Female C57/BL6 mice of 810weeks were used. EAE was induced by the subcutaneous application of 50g of myelin oligodendrocyte glycoprotein (MOG) peptide (amino acids: 3555) emulsified in 100L complete Freunds adjuvant (Difco Laboratories) and supplemented with 10mg/mL Mycobacterium tuberculosis, on the side of each hind flank. Pertussis toxin (200L of 0.5g/mL solution; List Biological Laboratories) was injected intraperitoneally (IP) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…To obtain cell culture supernatants containing soluble native IL-2, equine PBMC were either stimulated with PMA and ionomycin as described above but without an inhibitor of protein secretion, or with LPS E. coli O55:B5 at 12.5 g/ml (List Biological Laboratories, Campbell …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Experimental autoimmune encephalomyelitis (EAE):

… In addition, 100 ng/mouse of Pertussis toxin (List Biological Laboratories) were injected i.p. at days 0 and 2 of EAE induction. Mice were monitored daily for clinical disease symptoms according to the following scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…three consecutive days on the first week and once per week during the following 4 weeks, with the equivalent of 5 mg of protein per mouse of WP (two groups, n = 6/group) or HWP (two groups, n = 6/group) in PBS, plus 10 µg of cholera toxin (CT) (List Biologicals, Campbell …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE Model:

EAE was induced by s.c. injection of 200 g of myelin oligodendrocyte glycoprotein (MOG)₃₅₅₅ (GenScript) with IFA (Difco) plus 500 g nonviable, desiccated Mycobacterium tuberculosis (Difco) into the hind flank. On the day of and 2 d after immunization, 200 ng of pertussis toxin (List Biological) was injected intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Subjects and experimental outline:

In total, 40 mice (both genders) were used in this experiment with 20 each assigned to the control (Cre) and experimental (Cre⁺) groups. All subjects in both groups received one intraperitoneal injection (i.p.) of DT at a dose of 25 ng/g (List Biological Laboratories, Campbell, CA, USA) at postnatal day 21. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae