Bacterial Toxin Research Citations

Murine EAE model:

… EAE was induced in female WT and Abcc8/ mice at 8 weeks of age, as described [13]. … Each mouse then received 400 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) intraperitoneally (IP) on post-induction day (pid) 0 and pid-2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Patch-clamp recording and photoactivation:

… For blocking exocytosis, 200nM TeTx (List Biological Laboratories) along with Alexa568 was included in the internal solution. …

• Product #190 – Tetanus Toxin from Clostridium tetani

Adjuvanticity studies in rabbits:

All immunizations were performed in accordance with University of Minnesota IACUC guidelines
(Protocol 1601-33398A) by Envigo (Huntington, UK). Cohorts of 4 female New Zealand white
rabbits were immunized intramuscularly in the flank with 10 g antigen (diphtheria toxin mutant
CRM197, List Biological Laboratories, Campbell, CA)147-148 and 100 g adjuvant in 0.2 mL saline,
or antigen alone as published previously by us 171. …

• Product #149A – Diphtheria Toxin CRM197, Mutant

Healthy subjects provided fasting whole blood samples that were cultured on 96-well plates and treated for 6 hours with the following Toll-like receptor (TLR) agonists ex vivo: Pam3CSK4 (TLR1/2 agonist) 10ng/ml (EMC Microcollections GmbH, Tubingen, Germany), Yersinia pestis grown at 37C LPS (TLR4 agonist) 0.1 ng/ml (gift of Dr. R.K. Ernst, University of Washington), ultrapure Salmonella minnesota Re595 LPS (TLR4) 0.1 ng/ml (for cytokine assays) and 1.0 ng/ml (for cytokine assays and transcriptional assays) (List Biological Laboratories, Inc., Campbell, CA), …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

EAE induction and scoring:

… On the day of immunization and 2 days after, mice received 200 ng pertussis toxin (PTx) (List Biological Laboratories) in 100 l PBS i.v. Mice were checked daily and scored for EAE clinical signs as described elsewhere …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cytokine Detection Assay:

…As a positive control for induction of pro-inflammatory cytokines, 20 g/mL lipopolysaccharide (LPS; List Biological Labs, USA) from Escherichia coli was used.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents:

… PTX was purchased from List Biological Laboratories (Campbell, CA, USA). …

Impedance Measurement by ECIS:

Barrier function of EC junctions was evaluated using ECIS-z (Applied Biophysics, Jordan Road, Troy, NY), as described previously (Ando et al., 2013). In brief, we used 8W10E electrodes with a thin gold film surface and coated each well containing gold film electrodes with collagen so that the MS-1 cells could adhere to them. 4  10⁴ MS-1 cells were plated on electrodes and cultured overnight. Each well has a substrate area of 0.8 cm² and electrode area of 1.96 mm². The cells were then starved in 0.1% BSA-containing DMEM for 4 hr and subjected to ECIS. Resistance between cells (Rb) was measured in real time at 37C with 5% CO₂ using an ECIS-z system at 4,000; 16,000; and 64,000 Hz. MS-1 cells were pretreated for 24 hr with 100 ng/mL PTX to inhibit Gi.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 … Pertussis toxin (300 ng pertussis toxin in PBS; List Biological) was injected ip at the time of induction, and a second dose was administered 3 d later. The same volume of CFA and pertussis toxin was injected into each mouse in the control group. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The retrograde tracer cholera toxin subunit (CTB; List Biological Laboratories, Campbell, CA, USA) was injected by iontophoresis into the region of CEA of 23 animals. … CTB. Purified CTB isolated from Vibrio cholerae. List Biological Laboratories, goat, polyclonal, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

…For EAE induction, animals were immunized with 60 g MOG35-55 emulsified (1:1) in incom- plete Freund’s adjuvant (IFA) [11-15]. Pertussis toxin (200 ng; PTX, List Laboratories, Campbell, CA) was injected intraperitoneally on the day of immunization and 3 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)