Bacterial Toxin Research Citations

EAE induction and treatment:

EAE model was induced as previously described [41]. Briefly, Female mice at 8-10 wk of age were immunized subcutaneously in the flanks with 200 µg of MOG_35-55 peptides (purity >98%, GL Biochem Ltd., Shanghai) which were emulsified in complete Freunds adjuvant (CFA) containing 500 µg of Mycobacterium tuberculosis H37RA (Difco Laboratories, USA). Furthermore, 400 ng of pertussis toxin (PTX, List Biological Laboratories, USA) in 100 µl PBS-50 mM NaCl was intraperitoneally injected on the day of immunization and 24 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Electrophysiology:

… For the inflammatory model in adult DRG cultures, trinitrophenyl conjugated-lipopolysaccharide (TNP-LPS) conjugate from Escherichia coli (List Biological Laboratories, Campbell, CA, USA) was resuspended in media 1mg/mL and added to cultures (20g/mL). …

Author has indicated the following LPS used:

• Product #201 – LPS from Escherichia coli O111:B4

This paper provides valuable information regarding effects of ACT on phagocytes.

• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

… LPS from E. coli O111:B4 was purchased from List Biological Laboratories, Inc. …

• Product #201 – LPS from Escherichia coli O111:B4

… They also received intraperitoneal injections of pertussis toxin (300 ng in 100 µl PBS; List Biological Lab, Campbell, CA, USA) at the time of immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
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– … Pertussis toxin in HEC gel form was prepared according to the protocol described by Moench et al[10] Briefly, the lyophilized form of pertussis toxin (Cat # 180, List Biological Laboratories, Inc., Campbell, CA,) was resuspended in 1.5 mL of sterile water. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Pertussis toxin List Biological Laboratories Cat#180

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Macrophage tissue culture and RNA isolation:

… Murine bone marrow-derived macrophages (BMDMs) were cultured from female C57BL/6J mice (age 812 weeks) as previously described [9] and on day six, cells were re-plated into six-well tissue culture plates. On day seven, cells were incubated for the indicated times (see text in Results section) in complete RPMI with rhM-CSF and 10 ng/mL of LPS (from Salmonella enterica serovar minnesota R595; List Biological Laboratories, Campbell, CA) and then harvested. RNA was isolated using TRIzol (Thermo Fisher Scientific, Waltham, MA) following the manufacturers instructions. 

• Product #304 – LPS from Salmonella minnesota R595 (Re)

EAE:

… In addition, mice were given Pertussis toxin (Ptx) from List Biological Laboratories on days 0 and 2 postimmunization (75 and 200 ng per mouse, respectively). Immunized mice were scored (not blindly) for clinical signs of EAE graded on a six-point scale of combined hindlimb and forelimb paralysis scores (60). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Rats (n=3) were anesthetized with a surgical dose of KAX and received a unilateral LDTg injection of 100 nl of 0.25% cholera toxin B subunit (CTB; #104; List Laboratories) over 2 s, according to the following coordinates (according to Paxinos and Watson, 2005): …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt